selA

UniProt ID: Q88QJ8
Organism: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Review Status: COMPLETE
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Gene Description

SelA is the cytoplasmic, pyridoxal-5'-phosphate-dependent L-seryl-tRNA(Sec) selenium transferase of Pseudomonas putida KT2440. It uses selenophosphate supplied by SelD to convert Ser-tRNA(Sec) into Sec-tRNA(Sec), completing the bacterial charged-tRNA biosynthetic step that precedes SelB-dependent translation.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0001514 selenocysteine incorporation
IEA
GO_REF:0000120 		MODIFY
Summary: SelA makes Sec-tRNA(Sec) but does not itself execute ribosomal Sec insertion.
Reason: The direct SelA reaction is conversion of Ser-tRNA(Sec) to Sec-tRNA(Sec). GO:0001717 records that bounded biosynthetic step without assigning SelA the downstream SelB/ribosome incorporation event.
Supporting Evidence:
file:PSEPK/selA/selA-uniprot.txt
Converts seryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec)
GO:0001717 conversion of seryl-tRNAsec to selenocys-tRNAsec
IEA
GO_REF:0000104 		ACCEPT
Summary: This is the direct biological process catalyzed by bacterial SelA.
Reason: UniProt records the one-step bacterial route from Ser-tRNA(Sec) and selenophosphate to Sec-tRNA(Sec), and PANTHER assigns Q88QJ8 to the SelA subfamily.
Supporting Evidence:
file:PSEPK/selA/selA-uniprot.txt
selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec)
GO:0004125 L-seryl-tRNA(Sec) selenium transferase activity
IEA
GO_REF:0000120 		ACCEPT
Summary: This term precisely represents the SelA selenium-transfer reaction.
Reason: The reviewed entry assigns EC 2.9.1.1 and a reaction consuming L-seryl-tRNA(Sec) plus selenophosphate to form L-selenocysteinyl-tRNA(Sec).
Supporting Evidence:
file:PSEPK/selA/selA-uniprot.txt
Reaction=L-seryl-tRNA(Sec) + selenophosphate
GO:0030170 pyridoxal phosphate binding
IEA
file:PSEPK/selA/selA-uniprot.txt 		NEW
Summary: PLP binding is valid cofactor information for SelA.
Reason: The exact Q88QJ8 record assigns pyridoxal 5'-phosphate and a PLP-linked active-site lysine, but the fetched GOA snapshot lacks the corresponding cofactor-binding annotation.
Supporting Evidence:
file:PSEPK/selA/selA-uniprot.txt
Name=pyridoxal 5'-phosphate
GO:0005737 cytoplasm
IEA
GO_REF:0000120 		ACCEPT
Summary: A cytoplasmic location is consistent with soluble bacterial Sec-tRNA synthesis.
Reason: UniProt explicitly assigns SelA to the cytoplasm and predicts no membrane or export features.
Supporting Evidence:
file:PSEPK/selA/selA-uniprot.txt
SUBCELLULAR LOCATION: Cytoplasm

Core Functions

PLP-dependent transfer of activated selenium from selenophosphate to Ser-tRNA(Sec), producing Sec-tRNA(Sec) for subsequent SelB-mediated translation.

Molecular Function:
L-seryl-tRNA(Sec) selenium transferase activity
Directly Involved In:
conversion of seryl-tRNAsec to selenocys-tRNAsec
Cellular Locations:
cytoplasm
Supporting Evidence:
  • file:PSEPK/selA/selA-uniprot.txt
    Converts seryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec)
  • file:PSEPK/selA/selA-deep-research-openscientist.md
    **SelA (PP_0493) is a pyridoxal-5′-phosphate (PLP)-dependent selenocysteine synthase (EC 2.9.1.1) that catalyzes the terminal, committed step of selenocysteine (Sec, the 21st amino acid) biosynthesis in the bacterial cytoplasm.**

References

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Suggested Experiments

Experiment: Reconstitute the reaction with purified SelA, Ser-tRNA(Sec), and selenophosphate, and quantify Sec-tRNA(Sec) formation with and without PLP.

Hypothesis: Q88QJ8 converts KT2440 Ser-tRNA(Sec) to Sec-tRNA(Sec) using SelD-derived selenophosphate.

Deep Research

OpenScientist

(selA-deep-research-openscientist.md)

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📚 Additional Documentation

Notes

(selA-notes.md)

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