| Feature | Summary | Evidence / source |
|---|---|---|
| Target gene / protein | **sucC = PP_4186 = UniProt Q88FB2** in *Pseudomonas putida* KT2440; annotated as **succinate--CoA ligase [ADP-forming] / succinyl-CoA synthetase subunit beta**. A partner gene **sucD = PP_4185** is mentioned in operon/KEGG context as the other subunit of the complex. | Geiger 2019 identifies PP_4186 as the succinate-CoA ligase [ADP-forming] subunit beta and references KEGG **PP_4185 + PP_4186** for the complex (pqac-00000000, pqac-00000001) |
| Enzyme name / EC / reaction | The SucC/SucD complex is **succinyl-CoA synthetase / succinate--CoA ligase (ADP-forming), EC 6.2.1.5**. Core reaction: **succinyl-CoA + ADP + Pi ⇌ succinate + CoA + ATP**. | Explicit reaction and subunit assignment in *Pseudomonas* sucC literature; broader bacterial mechanistic reviews agree that SCS performs the substrate-level phosphorylation step of the TCA cycle (pqac-00000001, pqac-00000014, pqac-00000016) |
| Complex architecture | In bacteria, succinyl-CoA synthetase is composed of **alpha and beta subunits** (commonly discussed as **SucD/SucC**) and forms the canonical bacterial SCS complex. | Bacterial structural/biochemical summaries and *Pseudomonas* locus context support two-subunit organization (pqac-00000014, pqac-00000017, pqac-00000000) |
| Cellular location | **Likely cytosolic / soluble central-metabolic enzyme**, but **no retrieved source directly demonstrated localization for *P. putida* KT2440 sucC**. Localization should therefore be treated as inferred from its TCA-cycle role rather than directly proven here. | Retrieved evidence situates the enzyme in central carbon metabolism/TCA but does not provide a direct localization experiment for KT2440 (pqac-00000014, pqac-00000016) |
| Pathway context | **TCA cycle:** reversible succinyl-CoA ↔ succinate step coupled to ATP formation. **Additional 2024 systems-biology context:** in anoxic-electrogenic *P. putida*, the **acetate:succinate CoA-transferase (AST) pathway** is described as coupling ATP formation through regeneration of succinyl-CoA into succinate **via succinyl-CoA synthetase**. | Canonical TCA role and 2024 AST/acetate-coupling interpretation in KT2440 (pqac-00000014, pqac-00000004, pqac-00000020) |
| Regulatory / RNA context | A **sucC 5' UTR RNA motif** occurs uniquely in *Pseudomonas* species and was proposed as a cis-regulatory element; 72 representatives were reported. Geiger’s work also reported candidate interaction of succinate-CoA ligase subunits with this RNA motif. | RNA motif/regulatory context for *Pseudomonas* sucC (pqac-00000001, pqac-00000003, pqac-00000019) |
| Quantitative finding: multidrug-resistance link via SCS disruption | In KT2440-related work, a multidrug-resistant mutant **HPG-5** carried an inactivating mutation in **sucD**; second-type mutants like HPG-5 appeared at **1 × 10⁻⁹** selection frequency. Deleting **parXY (PP_3455/3456)** restored susceptibility with **8- to 16-fold MIC decreases** for gentamicin/amikacin and **2- to 4-fold** decreases for fluoroquinolones/tobramycin; deleting **ttgABC** sensitized KT2440 to β-lactams by **2- to 32-fold**, fluoroquinolones **2-fold**, chloramphenicol **2-fold**, tetracycline **2-fold**, novobiocin **32-fold**. The phenotype was linked to **sucD** disruption activating the glyoxylate shunt. | Quantitative antibiotic-resistance phenotypes from Puja et al. 2020 (pqac-00000006, pqac-00000011) |
| Quantitative finding: anoxic-electrogenic systems biology | Under anoxic-electrogenic conditions, KT2440 maintained selective metabolism; after 24 h, **95 proteins decreased** and **40 increased** significantly. After 100 h, abundance of **27 ribosomal proteins decreased**, with reductions of **up to 24-fold** (RpsU example). These data provide recent systems-level context in which succinyl-CoA synthetase participates in ATP-linked acetate routing. | Weimer et al. 2024 proteome/time-course data (pqac-00000007, pqac-00000004) |
| Quantitative finding: butanol assimilation / central metabolism context | In *P. putida* BIRD-1 (not KT2440, but relevant *Pseudomonas* central-metabolism context), an acyl-CoA synthetase candidate was induced **245-fold** and an adjacent acyl-CoA dehydrogenase-domain protein **278-fold** during butanol assimilation; the glyoxylate shunt was highlighted as key for routing carbon to central metabolism. | Strain-specific but informative *Pseudomonas* metabolic context; not direct evidence for KT2440 sucC expression (pqac-00000009) |
| Key references | **Geiger 2019** (RNA-based regulation thesis; Pseudomonas sucC motif and PP_4186 annotation; URL not available in retrieved context). **Puja et al. 2020**, *Environmental Microbiology*, DOI: https://doi.org/10.1111/1462-2920.15200. **Weimer et al. 2024**, *Microbial Cell Factories*, DOI: https://doi.org/10.1186/s12934-024-02509-8. **Lancaster & Graham 2023**, *Int J Mol Sci*, DOI: https://doi.org/10.3390/ijms241310725. **Nolte et al. 2014**, *Appl Environ Microbiol*, DOI: https://doi.org/10.1128/AEM.03075-13. **Schürmann et al. 2011**, *J Bacteriol*, DOI: https://doi.org/10.1128/JB.00049-11. | Consolidated from retrieved citation contexts (pqac-00000012, pqac-00000013, pqac-00000014, pqac-00000017) |


*Table: This table compiles the verified identity, biochemical role, pathway placement, and quantitative literature findings for *P. putida* KT2440 sucC (PP_4186/Q88FB2) and its partner subunit context. It is useful as a compact evidence map linking annotation, reaction chemistry, and recent systems-level observations.*