thrC

Gene Description

thrC (PP_1471) encodes threonine synthase (EC 4.2.3.1), a pyridoxal 5'-phosphate (PLP)-dependent enzyme that catalyzes the final step of L-threonine biosynthesis, the conversion of O-phospho-L-homoserine and water to L-threonine and inorganic phosphate (RHEA:10840). The enzyme belongs to the threonine synthase family within the fold-type II (TrpB-like) PLP-dependent enzyme superfamily, with PLP bound as a Schiff base to an active-site lysine. As the committed terminal step of the aspartate-derived threonine biosynthetic branch, thrC supplies L-threonine for protein synthesis and downstream isoleucine biosynthesis in P. putida.

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0004795 threonine synthase activity	IEA GO_REF:0000120	ACCEPT	Summary: Core molecular function. This is the EC 4.2.3.1 activity (O-phospho-L-homoserine + H2O -> L-threonine + phosphate, RHEA:10840) matching the UniProt RecName, NCBIfam TIGR00260 (thrC) and threonine synthase family assignment (IPR004450). Well supported despite IEA evidence.
GO:0006520 amino acid metabolic process	IEA GO_REF:0000002	MARK AS OVER ANNOTATED	Summary: Broad parent biological process term assigned by InterPro2GO from the Ser/Thr dehydratase PLP signature (IPR000634). It is correct but far less informative than the specific GO:0009088 (L-threonine biosynthetic process) that is also annotated. Marking as over-annotated relative to the precise child term.
GO:0009088 L-threonine biosynthetic process	IEA GO_REF:0000120	ACCEPT	Summary: Core biological process. Threonine synthase catalyzes the terminal step of L-threonine biosynthesis; supported by the UniProt pathway annotation (UPA00050/UER00065) and UniRule. Accept.
GO:0030170 pyridoxal phosphate binding	IEA GO_REF:0000002	ACCEPT	Summary: Supporting molecular function. Threonine synthase is PLP-dependent; the UniProt cofactor annotation lists pyridoxal 5'-phosphate and a Schiff-base lysine (MOD_RES at position 112) is documented. Accept.

Core Functions

PLP-dependent synthesis of L-threonine from O-phospho-L-homoserine, the terminal step of the aspartate-derived threonine biosynthetic pathway

Molecular Function:

threonine synthase activity

Directly Involved In:

L-threonine biosynthetic process

Supporting Evidence:

GO_REF:0000120
threonine synthase activity (EC:4.2.3.1, RHEA:10840) assigned to Q88MU7

Gene Description

Existing Annotations Review

Core Functions

PLP-dependent synthesis of L-threonine from O-phospho-L-homoserine, the terminal step of the aspartate-derived threonine biosynthetic pathway

References

Deep Research

Asta

📄 View Raw YAML