ureB

UniProt ID: Q88J05
Organism: Pseudomonas putida KT2440
Review Status: DRAFT
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Gene Description

UreB is the cytoplasmic beta structural subunit of the three-chain bacterial urease. It associates with UreA and the catalytic UreC chain in the UreABC apoprotein and contributes to urea hydrolysis by the mature dinickel complex.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0005737 cytoplasm
IEA
GO_REF:0000120 		ACCEPT
Summary: The reviewed UniProt record places UreB in the cytoplasm.
Reason: This is consistent with the soluble UreABC complex.
GO:0009039 urease activity
IEA
GO_REF:0000120 		MARK AS OVER ANNOTATED
Summary: UreB contributes structurally to the heteromeric urease activity.
Reason: UreB does not independently enable urease activity; it contributes to activity of UreABC, whose catalytic binickel site is in UreC (PMID:8718850).
GO:0035550 urease complex
IEA
GO_REF:0000002 		ACCEPT
Summary: UreB is the beta subunit of the UreABC urease complex.
Reason: Structural studies define bacterial urease as an alpha-beta-gamma assembly.
GO:0043419 urea catabolic process
IEA
GO_REF:0000120 		ACCEPT
Summary: UreB is a required structural component of the urea-hydrolyzing complex.
Reason: Mature UreABC directly hydrolyzes urea.
GO:0005198 structural molecule activity
ISS
PMID:8718850
Structures of the Klebsiella aerogenes urease apoenzyme and ... 		NEW
Summary: UreB supplies structural integrity to the heteromeric urease complex.
Reason: The beta chain is a noncatalytic structural subunit of the alpha-beta-gamma assembly.
Supporting Evidence:
PMID:8718850
Urease from Klebsiella aerogenes [Jabri et al. (1995) Science 268, 998-1004] is an (alpha beta gamma)3 trimer with each alpha-subunit having an (alpha beta)8-barrel domain containing a binickel active center.

Core Functions

Beta structural subunit contributing to urease activity in the UreABC complex.

Molecular Function:
structural molecule activity
Directly Involved In:
urea catabolic process
Cellular Locations:
cytoplasm
In Complex:
urease complex
Supporting Evidence:
  • PMID:8718850
    Urease from Klebsiella aerogenes [Jabri et al. (1995) Science 268, 998-1004] is an (alpha beta gamma)3 trimer with each alpha-subunit having an (alpha beta)8-barrel domain containing a binickel active center.

References

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Suggested Questions for Experts

Q: Has loss of PP_2844 been shown to disrupt KT2440 UreABC assembly or activity?

Suggested Experiments

Experiment: Measure complex assembly and urea hydrolysis in a ureB deletion/complementation series.

Hypothesis: Q88J05 is required structurally for active KT2440 UreABC.

Type: Gene deletion, complementation, native complex analysis, and urease assay

📚 Additional Documentation

Notes

(ureB-notes.md)

ureB curation notes

2026-08-11

  • Q88J05 is reviewed UniProt UreB/PP_2844, the 105-aa beta structural chain of
    the UreABC complex [ureB-uniprot.txt, "Urease subunit beta"].
  • The historical review at 86cf4fd8e9 was used only for comparison. Its
    complex membership survives current review, but the individual enables
    qualifier for urease activity does not.
  • Structural evidence places urease chemistry in the alpha/UreC binickel site,
    while UreB is part of the alpha-beta-gamma assembly
    [PMID:8718850, "Urease ... is an (alpha beta gamma)3 trimer"].
  • Q88J05 is curated as a contributor to GO:0009039, a member of
    GO:0035550, and a direct participant in urea catabolism.

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