ureC

UniProt ID: Q88J04
Organism: Pseudomonas putida KT2440
Review Status: DRAFT
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Gene Description

UreC is the cytoplasmic alpha and catalytic subunit of the three-chain bacterial urease. Within the UreABC complex it supplies the carbamylated lysine and ligands of the dinickel active site that hydrolyzes urea to ammonia and carbon dioxide after UreDEFG-dependent maturation.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0005737 cytoplasm
IEA
GO_REF:0000120 		ACCEPT
Summary: UreC is a cytoplasmic subunit of soluble bacterial urease.
Reason: The reviewed UniProt localization is coherent with the UreABC complex.
GO:0009039 urease activity
IEA
GO_REF:0000120 		ACCEPT
Summary: UreC is the catalytic subunit that contains the complete dinickel urease active site.
Reason: Requiring assembly with UreA and UreB does not make UreC a noncatalytic subunit. Structural evidence places the complete catalytic center in the UreC-equivalent alpha chain (PMID:8718850).
GO:0016151 nickel cation binding
IEA
GO_REF:0000120 		ACCEPT
Summary: UreC contains the urease dinickel catalytic center.
Reason: The alpha-subunit binickel site is directly established structurally (PMID:8718850).
GO:0016787 hydrolase activity
IEA
GO_REF:0000002 		KEEP AS NON CORE
Summary: This is a broad parent of the supported urease activity.
Reason: The ancestor is biologically true, but GO:0009039 is the informative reaction-specific function.
GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
IEA
GO_REF:0000002 		KEEP AS NON CORE
Summary: This carbon-nitrogen hydrolase parent is compatible but less precise than urease activity.
Reason: This ancestor is biologically true, while GO:0009039 captures the exact EC 3.5.1.5 reaction.
GO:0043419 urea catabolic process
IEA
GO_REF:0000120 		ACCEPT
Summary: UreC provides the active site for the urea-catabolic reaction.
Reason: Mature urease directly hydrolyzes urea.
GO:0035550 urease complex
IEA
file:PSEPK/ureC/ureC-uniprot.txt 		NEW
Summary: UreC is the catalytic alpha subunit of the UreABC urease complex.
Reason: Reviewed UniProt and alpha-beta-gamma structural evidence support explicit complex membership.

Core Functions

Catalytic alpha subunit that enables urease activity within the UreABC complex.

Molecular Function:
urease activity
Directly Involved In:
urea catabolic process
Cellular Locations:
cytoplasm
In Complex:
urease complex
Supporting Evidence:
  • PMID:8718850
    Urease from Klebsiella aerogenes [Jabri et al. (1995) Science 268, 998-1004] is an (alpha beta gamma)3 trimer with each alpha-subunit having an (alpha beta)8-barrel domain containing a binickel active center.

Binds the two nickel ions of the UreC catalytic center.

Molecular Function:
nickel cation binding
Cellular Locations:
cytoplasm
In Complex:
urease complex
Supporting Evidence:
  • PMID:8718850
    Urease from Klebsiella aerogenes [Jabri et al. (1995) Science 268, 998-1004] is an (alpha beta gamma)3 trimer with each alpha-subunit having an (alpha beta)8-barrel domain containing a binickel active center.

References

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Suggested Questions for Experts

Q: Is Q88J04 active-site occupancy and carbamylation nickel-limited in KT2440?

Suggested Experiments

Experiment: Purify KT2440 UreABC and quantify UreC nickel occupancy, lysine carbamylation, and urea-hydrolysis kinetics before and after UreDEFG activation.

Hypothesis: UreDEFG increases Q88J04 lysine carbamylation and dinickel occupancy to generate active UreABC.

Type: Native complex purification, metal analysis, mass spectrometry, and enzyme kinetics

📚 Additional Documentation

Notes

(ureC-notes.md)

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