vacJ

UniProt ID: Q88KX6
Organism: Pseudomonas putida KT2440
Review Status: COMPLETE
📝 Provide Detailed Feedback

Gene Description

Outer-membrane VacJ/MlaA-family lipoprotein that forms the outer-membrane phospholipid-handling interface of the Mla system and participates in intermembrane phospholipid transfer and maintenance of envelope lipid organization.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0016020 membrane
IEA
GO_REF:0000002 		MODIFY
Summary: Correct but too broad for an outer-membrane MlaA-family lipoprotein.
Reason: MlaA/VacJ is an outer-membrane component, so GO:0009279 is the informative bacterial membrane location.
Proposed replacements: cell outer membrane
Supporting Evidence:
file:PSEPK/vacJ/vacJ-deep-research-openscientist.md
Located within the outer membrane, MlaA (VacJ) acts as a channel to shuttle phospholipids from the outer leaflet
GO:0120010 intermembrane phospholipid transfer
IEA
GO_REF:0000118 		ACCEPT
Summary: Correct process annotation for the outer-membrane Mla interface.
Reason: Conserved MlaA/VacJ family function places Q88KX6 at the outer-membrane interface of intermembrane phospholipid transfer.

Core Functions

Outer-membrane phospholipid handling in the Mla intermembrane transfer system. Support is MlaA family classification (Pfam MlaA, InterPro IPR007428) plus a SignalP-predicted signal peptide (1..31) consistent with export to the envelope; the outer-membrane MlaA-OmpC/F role is from the homologous Mla system (PMID:33236984), not a KT2440 experiment.

Directly Involved In:
intermembrane phospholipid transfer
Cellular Locations:
cell outer membrane
Supporting Evidence:
  • file:PSEPK/vacJ/vacJ-uniprot.txt
    DR Pfam; PF04333; MlaA; 1.
  • file:PSEPK/vacJ/vacJ-uniprot.txt
    DR InterPro; IPR007428; MlaA.
  • file:PSEPK/vacJ/vacJ-uniprot.txt
    FT SIGNAL 1..31
  • PMID:33236984
    (2) an OM complex, MlaA-OmpC/F

References

Loading supporting content…

Download this section (compressed HTML)

Suggested Questions for Experts

Q: Does KT2440 VacJ/MlaA preferentially extract or receive specific outer-membrane phospholipid species?

Q: Is PP_2163 lipidated, and does it form an outer-membrane complex with a specific KT2440 porin?

Suggested Experiments

Experiment: Reconstitute VacJ/MlaA in asymmetric outer-membrane mimetics and quantify phospholipid exchange with Ttg2D.

Type: asymmetric membrane lipid-transfer assay

Deep Research

OpenScientist

(vacJ-deep-research-openscientist.md)

Loading supporting content…

Download this section (compressed HTML)

📚 Additional Documentation

Notes

(vacJ-notes.md)

Loading supporting content…

Download this section (compressed HTML)

📄 View Raw YAML

Loading supporting content…

Download this section (compressed HTML)