xseB

UniProt ID: Q88QG5
Organism: Pseudomonas putida KT2440
Review Status: DRAFT
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Gene Description

XseB is the small subunit of the exodeoxyribonuclease VII complex. It assembles with XseA to support bidirectional single-stranded DNA degradation to nucleoside 5'-phosphates. The complex is a plausible orientation-flexible nuclease for mismatch-excision tracts, but its specific participation in KT2440 mismatch repair remains untested.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0005737 cytoplasm
IEA
GO_REF:0000120 		MARK AS OVER ANNOTATED
Summary: True but redundant with the more specific cytosol annotation on the same protein.
Reason: Retain one nonredundant leaf-level location.
GO:0005829 cytosol
IEA
GO_REF:0000118 		ACCEPT
Summary: Accept as the specific location of the soluble Exonuclease VII subunit.
Reason: Avoids retaining both cytoplasm and its cytosol child.
GO:0006308 DNA catabolic process
IEA
GO_REF:0000120 		ACCEPT
Summary: Accept as the direct output of the XseA/XseB exonuclease.
Reason: Exonuclease VII degrades single-stranded DNA.
GO:0008855 exodeoxyribonuclease VII activity
IEA
GO_REF:0000120 		ACCEPT
Summary: Accept as the complex-level activity to which the XseB small subunit contributes.
Reason: Exact EC 3.1.11.6 family assignment in a reviewed UniProt record.
Supporting Evidence:
file:PSEPK/xseB/xseB-uniprot.txt
EC=3.1.11.6
GO:0009318 exodeoxyribonuclease VII complex
IEA
GO_REF:0000002 		ACCEPT
Summary: Accept. XseB is the small subunit of the XseA/XseB heterooligomer.
Reason: Directly supported by the reviewed subunit annotation.
Supporting Evidence:
file:PSEPK/xseB/xseB-uniprot.txt
Heterooligomer composed of large and small subunits.

Core Functions

XseB is the small subunit of exodeoxyribonuclease VII and contributes to bidirectional degradation of single-stranded DNA with XseA.

Directly Involved In:
DNA catabolic process
Cellular Locations:
cytosol
In Complex:
exodeoxyribonuclease VII complex
Supporting Evidence:
  • file:PSEPK/xseB/xseB-uniprot.txt
    Heterooligomer composed of large and small subunits.
  • file:PSEPK/xseB/xseB-uniprot.txt
    EC=3.1.11.6
  • file:PSEPK/xseB/xseB-deep-research-openscientist.md
    XseB is a **non-catalytic, architectural/stabilizing subunit** that decorates and stabilizes the central Ξ±-helical spine of XseA.

References

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Suggested Questions for Experts

Q: Does XseB control Exonuclease VII stability or substrate choice during DNA repair in KT2440?

Suggested Experiments

Experiment: Compare XseA abundance, complex assembly, and bidirectional ssDNA exonuclease activity in wild type and an xseB deletion.

Hypothesis: XseB is required for stable, active KT2440 Exonuclease VII.

Type: complex assembly and nuclease assay

Deep Research

OpenScientist

(xseB-deep-research-openscientist.md)

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πŸ“š Additional Documentation

Notes

(xseB-notes.md)

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πŸ“„ View Raw YAML

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