Mitochondrial manganese/iron superoxide dismutase (Mn/Fe-SOD) from the tardigrade Ramazzottius varieornatus. This is a member of the Mn/Fe-SOD family (InterPro IPR001189, IPR019831, IPR019832, IPR019833) - a completely different protein family from the Cu/Zn-SODs that constitute most of the R. varieornatus SOD repertoire. Mn/Fe-SODs are typically located in mitochondria (in eukaryotes) and use a single Mn or Fe ion for catalysis, unlike Cu/Zn-SODs which use a Cu+Zn binuclear center. The protein is predicted to be located in mitochondrion and may be part of a respiratory chain complex (UniRule annotations). 230 aa with an N-terminal extension consistent with a mitochondrial targeting peptide. NOTE: Earlier bioinformatic analysis incorrectly compared this protein to human SOD1 (Cu/Zn-SOD) and found low sequence conservation - this was because they belong to different protein families, NOT because RvY_01767 is degraded.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004784 superoxide dismutase activity | IEA GO_REF:0000120 | ACCEPT | Summary: Standard automated annotation for the Mn/Fe-SOD family. The protein belongs to the Mn/Fe-SOD InterPro families (IPR001189, IPR019831, IPR019832, IPR019833), which is a distinct protein family from Cu/Zn-SOD. This annotation is appropriate for canonical Mn/Fe-SODs and we have no evidence to suggest this protein has lost catalytic function. Reason: Family-based assignment is appropriate for an unreviewed Mn/Fe-SOD from a non-model organism with no experimental data. |
| GO:0005739 mitochondrion | IEA GO_REF:0000120 | ACCEPT | Summary: Standard subcellular location for eukaryotic Mn-SOD (homologous to human SOD2). The protein has an N-terminal extension consistent with a mitochondrial targeting peptide. |
| GO:0006801 superoxide metabolic process | IEA GO_REF:0000002 | ACCEPT | Summary: Standard biological process annotation for SOD enzymes. |
| GO:0019430 removal of superoxide radicals | IEA GO_REF:0000108 | ACCEPT | Summary: More specific child of GO:0006801, inferred from SOD activity. |
| GO:0030145 manganese ion binding | IEA GO_REF:0000118 | ACCEPT | Summary: Mn-SOD requires manganese as catalytic cofactor. This annotation is consistent with the Mn/Fe-SOD family assignment. |
| GO:0046872 metal ion binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: Parent term of GO:0030145 (manganese ion binding). Redundant with the more specific annotation. |
| GO:0098803 respiratory chain complex | IEA GO_REF:0000117 | REMOVE | Summary: This annotation comes from an ARBA rule (ARBA00029130) and is unusual for a Mn-SOD. Mitochondrial Mn-SOD (SOD2) is typically located in the mitochondrial matrix as a soluble enzyme, NOT as part of the respiratory chain complex. The respiratory chain consists of complexes I-IV plus ATP synthase, none of which contain SOD as a constituent. This appears to be an over-annotation by the ARBA rule. Reason: Mitochondrial Mn-SOD is a soluble matrix protein, not part of the respiratory chain complex. The ARBA rule appears to be incorrect for this annotation. |
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