RvY_03754

Gene Description

Putative Cu/Zn superoxide dismutase paralog from R. varieornatus, one of approximately 10 CuZnSOD-family proteins encoded by this extremotolerant tardigrade. Bioinformatic analysis (file:RAMVA/RvY_13070/RvY_13070-bioinformatics/RESULTS.md) shows that all four canonical Cu-binding histidines, all four Zn-binding residues, and both intrachain disulfide cysteines are preserved at the sequence level. The protein matches both PROSITE Cu/Zn SOD signatures (PS00087 for the N-terminal Cu coordination loop and PS00332 for the C-terminal disulfide region), as well as the Pfam SODC family (PF00080). This is consistent with canonical Cu/Zn superoxide dismutase activity, although biochemical confirmation has not been published for this specific paralog. 156 aa, 63% identity to human SOD1; all residues and PROSITE patterns match canonical CuZnSOD

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0004784 superoxide dismutase activity	IEA GO_REF:0000120	ACCEPT	Summary: All catalytic residues (4 Cu His, 4 Zn ligands, 2 disulfide Cys) are preserved at the sequence level. PROSITE PS00087 (N-terminal Cu coordination signature) and PS00332 (C-terminal disulfide signature) both match. The protein is in Pfam family PF00080 (Sod_Cu). Canonical SOD activity is plausible based on sequence and motif analysis, though biochemical data are lacking for this specific paralog. ACCEPT with the caveat that confidence is limited to sequence-level inference. Reason: All sequence-level criteria for canonical CuZnSOD are met. Supporting Evidence: file:RAMVA/RvY_13070/RvY_13070-bioinformatics/RESULTS.md RvY_03754 \| A0A1D1UP68 \| bioinformatic verdict: FUNCTIONAL
GO:0005507 copper ion binding	IEA GO_REF:0000002	ACCEPT	Summary: All four canonical Cu-binding histidines are preserved at the sequence level. Copper binding is likely.
GO:0006801 superoxide metabolic process	IEA GO_REF:0000002	ACCEPT	Summary: Inferred from SOD activity annotation.
GO:0019430 removal of superoxide radicals	IEA GO_REF:0000108	ACCEPT	Summary: Inferred from SOD activity annotation.
GO:0046872 metal ion binding	IEA GO_REF:0000002	KEEP AS NON CORE	Summary: Parent term of the more specific Cu/Zn binding annotations. Both Cu and Zn binding are likely.

Gene Description

Existing Annotations Review

References

Deep Research

Falcon

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