SPAC1851.02 (UniProt Q9US20; the fission yeast SLC1 ortholog) encodes an ER 1-acylglycerol-3-phosphate O-acyltransferase (LPAAT; EC 2.3.1.51), which acylates lysophosphatidic acid at sn-2 to make phosphatidic acid, the branch point for phospholipid and triacylglycerol synthesis. Function is assigned by orthology; it is in the ER in a genome-wide screen.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity | IBA GO_REF:0000033 | ACCEPT | Summary: LPA acyltransferase (AGPAT) activity (PAINT/EC; S. cerevisiae SLC1 ortholog). Reason: No S. pombe assay exists (UniProt still names it uncharacterized); the activity rests on membership of the 1-acyl-sn-glycerol-3-phosphate acyltransferase family (curated SIMILARITY, NCBIfam TIGR00530, InterPro IPR004552), whose characterized members include S. cerevisiae Slc1 and human AGPAT1/2 among the PAINT donors. The family-level argument is accepted for this ortholog. Supporting Evidence: file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt SIMILARITY: Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt NCBIfam; TIGR00530; AGP_acyltrn; 1. |
| GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity | IEA GO_REF:0000002 | ACCEPT | Summary: LPA acyltransferase (AGPAT) activity (PAINT/EC; S. cerevisiae SLC1 ortholog). Reason: The mapping rests on InterPro IPR004552 (1-acyl-sn-glycerol-3-phosphate acyltransferase) membership, which UniProt's curated family assignment confirms; the activity is inferred from the family, not assayed in S. pombe. Supporting Evidence: file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt InterPro; IPR004552; AGP_acyltrans. file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt SIMILARITY: Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. |
| GO:0005783 endoplasmic reticulum | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: ORFeome screen: ER. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005783 endoplasmic reticulum | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference; consistent. Reason: Root organelle term kept per policy; the claim holds. UniProt curates an ER membrane location from the S. pombe ORFeome localization study (PubMed:16823372); the membrane sub-compartment is carried by the GO:0005789 rows. Supporting Evidence: file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt SUBCELLULAR LOCATION: Endoplasmic reticulum membrane |
| GO:0005789 endoplasmic reticulum membrane | IC GO_REF:0000036 | ACCEPT | Summary: ER membrane (curator inference/mapping); consistent. Reason: Two independent lines agree, the ER localization from the ORFeome screen (curated by UniProt, PubMed:16823372) and three predicted transmembrane helices. Supporting Evidence: file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt SUBCELLULAR LOCATION: Endoplasmic reticulum membrane file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt TRANSMEM 38..58 |
| GO:0005789 endoplasmic reticulum membrane | IEA GO_REF:0000044 | ACCEPT | Summary: ER membrane (curator inference/mapping); consistent. Reason: The SubCell mapping comes from UniProt's curated ER membrane location (multi-pass membrane protein), tied to the ORFeome localization study (PubMed:16823372) and consistent with three predicted transmembrane helices. Supporting Evidence: file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt SUBCELLULAR LOCATION: Endoplasmic reticulum membrane file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt TRANSMEM 38..58 |
| GO:0006654 phosphatidic acid biosynthetic process | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference: LPA -> PA. Reason: Follows from the family-inferred LPAAT activity, which acylates lysophosphatidic acid to phosphatidic acid. The support is family membership (curated SIMILARITY, InterPro IPR004552), not an S. pombe experiment. Supporting Evidence: file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt SIMILARITY: Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt InterPro; IPR004552; AGP_acyltrans. |
| GO:0008654 phospholipid biosynthetic process | IEA GO_REF:0000002 | TOO GENERAL | Summary: Broad parent of the phosphatidic acid biosynthetic process annotated in this review. Reason: True but entailed by GO:0006654 (phosphatidic acid biosynthetic process), accepted elsewhere in this review. The basis is family membership (1-acyl-sn-glycerol-3-phosphate acyltransferase family; NCBIfam TIGR00530), not an S. pombe experiment. Supporting Evidence: file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt NCBIfam; TIGR00530; AGP_acyltrn; 1. |
| GO:0016020 membrane | IEA GO_REF:0000002 | TOO GENERAL | Summary: Generic membrane; the ER membrane location is annotated. Reason: True but entailed by GO:0005789 (endoplasmic reticulum membrane), accepted elsewhere in this review. The ER membrane location comes from the S. pombe genome-wide localisation screen (PMID:16823372) as recorded by UniProt, and the protein has predicted transmembrane helices. Supporting Evidence: file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:16823372}; Multi-pass membrane protein file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt TRANSMEM 1..21 |
| GO:0016746 acyltransferase activity | IEA GO_REF:0000002 | MODIFY | Summary: Generic InterPro-derived parent of the specific activity, which is already annotated. Reason: Correct but general. Family membership (1-acyl-sn-glycerol-3-phosphate acyltransferase family; NCBIfam TIGR00530 and InterPro IPR004552) supports the specific LPA acyltransferase activity GO:0003841, already annotated to this gene; no S. pombe assay. Proposed replacements: 1-acylglycerol-3-phosphate O-acyltransferase activity Supporting Evidence: file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt NCBIfam; TIGR00530; AGP_acyltrn; 1. file:SCHPO/SPAC1851.02/SPAC1851.02-uniprot.txt InterPro; IPR004552; AGP_acyltrans. |
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