SPAC1B3.01c (UniProt O13867; the fission yeast FUR1 ortholog) encodes uracil phosphoribosyltransferase (EC 2.4.2.9), which converts uracil and PRPP to UMP, the main route of uracil salvage. By orthology to S. cerevisiae FUR1, it is the enzyme that makes 5-fluorouracil toxic. Function is assigned by orthology; it is cytosolic in a genome-wide screen.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004845 uracil phosphoribosyltransferase activity | IEA GO_REF:0000120 | ACCEPT | Summary: EC/RHEA mapping of UPRT activity. Reason: UniProt curates the UPRT reaction (uracil + PRPP to UMP + diphosphate) by similarity (ECO:0000250), with PRPP- and GTP-binding residues transferred from the Toxoplasma UPRT (Q26998), and places the protein in the UPRTase family (Pfam UPRTase). Evidence is by similarity and family membership; there is no S. pombe assay. Supporting Evidence: file:SCHPO/SPAC1B3.01c/SPAC1B3.01c-uniprot.txt ribose 1-diphosphate (PRPP) to UMP and diphosphate. file:SCHPO/SPAC1B3.01c/SPAC1B3.01c-uniprot.txt Belongs to the UPRTase family. file:SCHPO/SPAC1B3.01c/SPAC1B3.01c-uniprot.txt PF14681; UPRTase |
| GO:0004845 uracil phosphoribosyltransferase activity | ISO GO_REF:0000024 | ACCEPT | Summary: Transfer from S. cerevisiae FUR1. Reason: ISO from S. cerevisiae FUR1. UniProt curates the UPRT reaction (uracil + PRPP to UMP + diphosphate) by similarity (ECO:0000250), with PRPP- and GTP-binding residues transferred from the Toxoplasma UPRT (Q26998), and places the protein in the UPRTase family (Pfam UPRTase). Evidence is by similarity and family membership; there is no S. pombe assay. Supporting Evidence: file:SCHPO/SPAC1B3.01c/SPAC1B3.01c-uniprot.txt ribose 1-diphosphate (PRPP) to UMP and diphosphate. file:SCHPO/SPAC1B3.01c/SPAC1B3.01c-uniprot.txt Belongs to the UPRTase family. file:SCHPO/SPAC1B3.01c/SPAC1B3.01c-uniprot.txt PF14681; UPRTase |
| GO:0005634 nucleus | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | KEEP AS NON CORE | Summary: ORFeome YFP screen recorded nuclear signal for SPAC1B3.01c. Soluble metabolic enzymes often show diffuse nucleocytoplasmic signal in this screen; no nuclear role is known. Reason: Experimental HDA kept, but not part of the core function. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005829 cytosol | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: ORFeome YFP screen placed SPAC1B3.01c in the cytosol, consistent with a soluble metabolic enzyme. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0006206 pyrimidine nucleobase metabolic process | IEA GO_REF:0000117 | TOO GENERAL | Summary: Broad parent. Reason: By similarity (UniProt ECO:0000250, no ortholog named) and UPRTase family membership: the protein converts uracil and PRPP to UMP, i.e. uracil salvage (GO:0006223) and UMP salvage (GO:0044206), both asserted elsewhere in this review; uracil salvage entails this broad pyrimidine nucleobase metabolism term. No S. pombe experiment. Supporting Evidence: file:SCHPO/SPAC1B3.01c/SPAC1B3.01c-uniprot.txt Catalyzes the conversion of uracil and 5-phospho-alpha-D- ribose 1-diphosphate (PRPP) to UMP and diphosphate. file:SCHPO/SPAC1B3.01c/SPAC1B3.01c-uniprot.txt Pyrimidine metabolism; UMP biosynthesis via salvage pathway; file:SCHPO/SPAC1B3.01c/SPAC1B3.01c-uniprot.txt Belongs to the UPRTase family. |
| GO:0006223 uracil salvage | IC GO_REF:0000111 | ACCEPT | Summary: Curator inference from UPRT activity: uracil salvage. Reason: UPRT converts free uracil and PRPP to UMP, the salvage step for uracil; UniProt curates this reaction by similarity and assigns the UniPathway step UMP from uracil in the salvage pathway. Rests on the by-similarity activity, not an S. pombe experiment. Supporting Evidence: file:SCHPO/SPAC1B3.01c/SPAC1B3.01c-uniprot.txt ribose 1-diphosphate (PRPP) to UMP and diphosphate. file:SCHPO/SPAC1B3.01c/SPAC1B3.01c-uniprot.txt Pyrimidine metabolism; UMP biosynthesis via salvage pathway; |
| GO:0008655 pyrimidine-containing compound salvage | IEA GO_REF:0000117 | TOO GENERAL | Summary: Broad ARBA parent. Reason: By similarity (UniProt ECO:0000250, no ortholog named) and UPRTase family membership: the uracil + PRPP to UMP reaction places the protein in uracil salvage (GO:0006223) and UMP salvage (GO:0044206), both asserted elsewhere in this review and both descendants of this broad salvage term. No S. pombe experiment. Supporting Evidence: file:SCHPO/SPAC1B3.01c/SPAC1B3.01c-uniprot.txt Catalyzes the conversion of uracil and 5-phospho-alpha-D- ribose 1-diphosphate (PRPP) to UMP and diphosphate. file:SCHPO/SPAC1B3.01c/SPAC1B3.01c-uniprot.txt Pyrimidine metabolism; UMP biosynthesis via salvage pathway; file:SCHPO/SPAC1B3.01c/SPAC1B3.01c-uniprot.txt Belongs to the UPRTase family. |
| GO:0044206 UMP salvage | IEA GO_REF:0000041 | ACCEPT | Summary: UniPathway mapping: UPRT makes UMP. Reason: The UniPathway assignment (UMP biosynthesis via salvage pathway, UMP from uracil) follows from the by-similarity UPRT activity curated in UniProt (uracil + PRPP to UMP) and UPRTase family membership; there is no S. pombe experiment. Supporting Evidence: file:SCHPO/SPAC1B3.01c/SPAC1B3.01c-uniprot.txt Pyrimidine metabolism; UMP biosynthesis via salvage pathway; file:SCHPO/SPAC1B3.01c/SPAC1B3.01c-uniprot.txt ribose 1-diphosphate (PRPP) to UMP and diphosphate. file:SCHPO/SPAC1B3.01c/SPAC1B3.01c-uniprot.txt Belongs to the UPRTase family. |
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