SPAC24C9.06c (aco1; UniProt O13966) encodes the mitochondrial aconitase (EC 4.2.1.3), a [4Fe-4S] hydratase that isomerises citrate to isocitrate via cis-aconitate, the second step of the TCA cycle. It is distinct from the aconitase-bL21m fusion aco2, whose essential role is in mitochondrial translation. It is mitochondrial in localisation studies.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003994 aconitate hydratase activity | IBA GO_REF:0000033 | ACCEPT | Summary: Aconitase activity (PAINT/EC); the main mitochondrial TCA aconitase. Reason: Curated UniProt function statement (by similarity, ECO:0000250, no named ortholog) plus membership of the mitochondrial-type aconitase family (NCBIfam TIGR01340 aconitase_mito; Pfam Aconitase), whose members catalyse citrate to isocitrate isomerisation. No S. pombe enzyme assay is cached; the activity rests on family classification and the conserved [4Fe-4S] aconitase domain. Supporting Evidence: file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt Catalyzes the isomerization of citrate to isocitrate via cis-aconitate, a step in the citric acid cycle. file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt DR NCBIfam; TIGR01340; aconitase_mito; 1. file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt DR Pfam; PF00330; Aconitase; 1. |
| GO:0003994 aconitate hydratase activity | IEA GO_REF:0000120 | ACCEPT | Summary: Aconitase activity (PAINT/EC); the main mitochondrial TCA aconitase. Reason: Curated UniProt function statement (by similarity, ECO:0000250, no named ortholog) plus membership of the mitochondrial-type aconitase family (NCBIfam TIGR01340 aconitase_mito; Pfam Aconitase), whose members catalyse citrate to isocitrate isomerisation. No S. pombe enzyme assay is cached; the activity rests on family classification and the conserved [4Fe-4S] aconitase domain. Supporting Evidence: file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt Catalyzes the isomerization of citrate to isocitrate via cis-aconitate, a step in the citric acid cycle. file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt DR NCBIfam; TIGR01340; aconitase_mito; 1. file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt DR Pfam; PF00330; Aconitase; 1. |
| GO:0005739 mitochondrion | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: ORFeome YFP screen: mitochondrion. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005739 mitochondrion | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference of mitochondrial location. Reason: Root organelle term. UniProt records a mitochondrial location with experimental evidence from the ORFeome YFP screen (ECO:0000269|PubMed:16823372), and the sequence carries a predicted N-terminal mitochondrial transit peptide (FT TRANSIT 1..32, ECO:0000255). The organelle claim holds. Supporting Evidence: file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:16823372}. file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt /note="Mitochondrion" |
| GO:0005739 mitochondrion | IDA PMID:25724335 Essential function of Aco2, a fusion protein of aconitase an... | ACCEPT | Summary: Mitochondrial aconitase localisation in the aco2 study (curator, full text). Supporting Evidence: PMID:25724335 A possible interaction between aconitase and a mitochondrial ribosomal protein was suggested in a genome-wide interactome study. |
| GO:0005739 mitochondrion | IEA GO_REF:0000120 | ACCEPT | Summary: UniProt/ARBA location mapping; consistent. Reason: Root organelle term. UniProt records a mitochondrial location with experimental evidence from the ORFeome YFP screen (ECO:0000269|PubMed:16823372), and the sequence carries a predicted N-terminal mitochondrial transit peptide (FT TRANSIT 1..32, ECO:0000255). The organelle claim holds. Supporting Evidence: file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:16823372}. file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt /note="Mitochondrion" |
| GO:0005759 mitochondrial matrix | IC GO_REF:0000111 | ACCEPT | Summary: Matrix location of this TCA enzyme (inference or mapping); consistent. Reason: Sub-compartment inferred from two lines: the experimentally recorded mitochondrial location with a predicted cleavable transit peptide (FT TRANSIT 1..32; no TRANSMEM feature in the record), and its by-similarity function as the citrate-isocitrate isomerase of the citric acid cycle, which runs in the matrix. No direct sub-mitochondrial assay for this protein is cached. Supporting Evidence: file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:16823372}. file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt /note="Mitochondrion" file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt Catalyzes the isomerization of citrate to isocitrate via cis-aconitate, a step in the citric acid cycle. |
| GO:0005829 cytosol | IBA GO_REF:0000033 | MARK AS OVER ANNOTATED | Summary: PAINT inference of a cytosolic pool, carried over from dual-localised aconitases such as S. cerevisiae Aco1; not shown for this S. pombe paralog, whose own localisation data are mitochondrial. Reason: Changed from KEEP_AS_NON_CORE, which asserts the term is true while the earlier reason said it was not established. The only S. pombe localisation evidence for this protein is mitochondrial (ORFeome screen, ECO:0000269|PubMed:16823372, plus a predicted transit peptide 1..32), and no S. pombe study reports a cytosolic pool of this paralog. In S. pombe the extramitochondrial aconitase pool reported so far belongs to the paralog Aco2 (PMID:25724335), so the cytosol IBA looks like over-propagation from dual-localised orthologs. Propagation Review Root cause: PROPAGATION BAD Failure modes: COMPARTMENT OR COMPLEX MISMATCH Sources checked: PANTHER:PTN001705783 SUPPORTS SOURCE BUT NOT TARGET The cytosolic pool is a property of dual-localised source aconitases (e.g. SGD:S000004295 Aco1); this S. pombe protein's own localisation data are mitochondrial only, and the reported extramitochondrial S. pombe aconitase is the paralog Aco2. Supporting Evidence: file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:16823372}. file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt /note="Mitochondrion" PMID:25724335 The bL21-fused Aco2 protein resides in mitochondria as well as in the cytosol and the nucleus. |
| GO:0006099 tricarboxylic acid cycle | EXP PMID:37164017 Optimization of energy production and central carbon metabol... | ACCEPT | Summary: 13C tracing in S. pombe versus S. japonicus shows that S. pombe runs a respiratory, oxidative TCA cycle (curator, full text). Supporting Evidence: PMID:37164017 Here, we use two related fission yeasts, Schizosaccharomyces pombe and Schizosaccharomyces japonicus, as a comparative model system. PMID:37164017 We show that although S. japonicus does not respire oxygen, unlike S. pombe, it is capable of efficient NADH oxidation, amino acid synthesis, and ATP generation. |
| GO:0006099 tricarboxylic acid cycle | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference; TCA cycle step. Reason: UniProt curated function (by similarity) and PATHWAY place the enzyme at the citrate to isocitrate step of the TCA cycle; family membership (TIGR01340 mitochondrial aconitase) supports this. S. pombe runs an oxidative TCA cycle (PMID:37164017, EXP row), but that study does not assay this enzyme specifically. Supporting Evidence: file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt Catalyzes the isomerization of citrate to isocitrate via cis-aconitate, a step in the citric acid cycle. file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt DR NCBIfam; TIGR01340; aconitase_mito; 1. |
| GO:0006099 tricarboxylic acid cycle | IEA GO_REF:0000120 | ACCEPT | Summary: InterPro/UniPathway mapping; consistent. Reason: UniProt curated function (by similarity) and PATHWAY place the enzyme at the citrate to isocitrate step of the TCA cycle; family membership (TIGR01340 mitochondrial aconitase) supports this. S. pombe runs an oxidative TCA cycle (PMID:37164017, EXP row), but that study does not assay this enzyme specifically. Supporting Evidence: file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt Catalyzes the isomerization of citrate to isocitrate via cis-aconitate, a step in the citric acid cycle. file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt DR NCBIfam; TIGR01340; aconitase_mito; 1. |
| GO:0051539 4 iron, 4 sulfur cluster binding | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: [4Fe-4S] cluster cofactor; subsidiary. Reason: [4Fe-4S] cluster binding is curated by similarity (ECO:0000250): one cluster per subunit, with cluster-ligating residues (e.g. 392, 455, 458) and the PROSITE aconitase signatures. No S. pombe experimental data. The cluster is the catalytic cofactor of the hydratase, so the term is true but subsidiary to aconitate hydratase activity. Supporting Evidence: file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250}; Note=Binds 1 [4Fe-4S] cluster per subunit. file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt InterPro; IPR018136; Aconitase_4Fe-4S_BS. |
| GO:0051539 4 iron, 4 sulfur cluster binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: [4Fe-4S] cluster cofactor; subsidiary. Reason: [4Fe-4S] cluster binding is curated by similarity (ECO:0000250): one cluster per subunit, with cluster-ligating residues (e.g. 392, 455, 458) and the PROSITE aconitase signatures. No S. pombe experimental data. The cluster is the catalytic cofactor of the hydratase, so the term is true but subsidiary to aconitate hydratase activity. Supporting Evidence: file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250}; Note=Binds 1 [4Fe-4S] cluster per subunit. file:SCHPO/SPAC24C9.06c/SPAC24C9.06c-uniprot.txt InterPro; IPR018136; Aconitase_4Fe-4S_BS. |
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