SPAC25B8.09 is a soluble methyltransferase type-11 protein found in the nucleus and cytosol. PomBase assigns trans-aconitate methyltransferase activity through curated orthology to budding-yeast Tmt1. This supports a probable small-molecule methyltransferase, while the predominant endogenous substrate and physiological role in fission yeast remain untested. Budding-yeast Tmt1 also methylates 3-isopropylmalate, so trans-aconitate detoxification is not the only plausible metabolic context.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005634 nucleus | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: SPAC25B8.09 is a soluble nuclear/cytosolic methyltransferase. Reason: The target HDA localization and experiment-attributed UniProt record agree. The localization atlas is abstract-only in the cache; accept the curated target observation without inventing a localization mechanism or substrate. Supporting Evidence: file:SCHPO/SPAC25B8.09/SPAC25B8.09-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus CC {ECO:0000269|PubMed:16823372}. |
| GO:0005634 nucleus | IEA GO_REF:0000044 | ACCEPT | Summary: SPAC25B8.09 is a soluble nuclear/cytosolic methyltransferase. Reason: The target HDA localization and experiment-attributed UniProt record agree. The localization atlas is abstract-only in the cache; accept the curated target observation without inventing a localization mechanism or substrate. Supporting Evidence: file:SCHPO/SPAC25B8.09/SPAC25B8.09-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus CC {ECO:0000269|PubMed:16823372}. |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | ACCEPT | Summary: SPAC25B8.09 is a soluble nuclear/cytosolic methyltransferase. Reason: The target HDA localization and experiment-attributed UniProt record agree. The localization atlas is abstract-only in the cache; accept the curated target observation without inventing a localization mechanism or substrate. Supporting Evidence: file:SCHPO/SPAC25B8.09/SPAC25B8.09-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus CC {ECO:0000269|PubMed:16823372}. |
| GO:0005829 cytosol | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: SPAC25B8.09 is a soluble nuclear/cytosolic methyltransferase. Reason: The target HDA localization and experiment-attributed UniProt record agree. The localization atlas is abstract-only in the cache; accept the curated target observation without inventing a localization mechanism or substrate. Supporting Evidence: file:SCHPO/SPAC25B8.09/SPAC25B8.09-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus CC {ECO:0000269|PubMed:16823372}. |
| GO:0008168 methyltransferase activity | IBA GO_REF:0000033 | MODIFY | Summary: The methyltransferase uses S-adenosylmethionine. Reason: The type-11 methyltransferase domain and experimentally grounded Tmt1 orthology support the donor-specific activity, without assigning one universal substrate across the broad family. Proposed replacements: S-adenosylmethionine-dependent methyltransferase activity Supporting Evidence: file:SCHPO/SPAC25B8.09/SPAC25B8.09-uniprot.txt DR InterPro; IPR013216; Methyltransf_11. PMID:11695919 We have identified the yeast open reading frame YER175c as the gene encoding the trans-aconitate methyltransferase of Saccharomyces cerevisiae. |
| GO:0008757 S-adenosylmethionine-dependent methyltransferase activity | IEA GO_REF:0000002 | ACCEPT | Summary: The type-11 protein is a SAM-dependent methyltransferase. Reason: Domain architecture and curated Tmt1 orthology support the catalytic class; these do not establish the dominant in vivo methyl acceptor. Supporting Evidence: file:SCHPO/SPAC25B8.09/SPAC25B8.09-uniprot.txt DR InterPro; IPR013216; Methyltransf_11. PMID:11695919 We have identified the yeast open reading frame YER175c as the gene encoding the trans-aconitate methyltransferase of Saccharomyces cerevisiae. |
| GO:0034198 cellular response to amino acid starvation | ISO GO_REF:0000024 | UNDECIDED | Summary: Conservation of the budding-yeast amino-acid-starvation response is unresolved. Reason: The transfer is from experimentally characterized Tmt1, but conservation of small-molecule methylation does not establish conserved regulation or invasive-growth signaling in fission yeast. No target-specific experiment resolving this physiological transfer was found. Supporting Evidence: PMID:11695919 We have identified the yeast open reading frame YER175c as the gene encoding the trans-aconitate methyltransferase of Saccharomyces cerevisiae. PMID:15147181 the major methylated endogenous product of Tmt1 in yeast extracts |
| GO:0046547 trans-aconitate 3-methyltransferase activity | ISO GO_REF:0000024 | ACCEPT | Summary: Trans-aconitate methyltransferase activity is supported as a curated orthology inference. Reason: PomBase’s ISO assignment explicitly traces to TMT1/SGD:S000000977, whose enzyme activity is experimentally characterized. Accept the molecular capability as a reasonable orthology inference while distinguishing it from a target assay or the claim that trans-aconitate is the dominant endogenous substrate. Supporting Evidence: PMID:11695919 We have identified the yeast open reading frame YER175c as the gene encoding the trans-aconitate methyltransferase of Saccharomyces cerevisiae. file:SCHPO/SPAC25B8.09/SPAC25B8.09-uniprot.txt DR InterPro; IPR013216; Methyltransf_11. file:SCHPO/SPAC25B8.09/SPAC25B8.09-P32643-ortholog-uniprot.txt DR SGD; S000000977; TMT1. |
| GO:1990748 cellular detoxification | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Detoxification is a plausible non-core consequence of trans-aconitate methylation. Reason: The curated transfer traces to Tmt1, but even the donor has an additional major endogenous substrate, 3-isopropylmalate. Retain the biological possibility without asserting detoxification as the sole or established physiological purpose in fission yeast. Supporting Evidence: PMID:11695919 We have identified the yeast open reading frame YER175c as the gene encoding the trans-aconitate methyltransferase of Saccharomyces cerevisiae. PMID:15147181 the major methylated endogenous product of Tmt1 in yeast extracts |
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