SPAC3A11.07

Summary: Generic NADH dehydrogenase; the specific non-electrogenic NADH:ubiquinone activity is annotated.

Reason: True but entailed by GO:0120555 (NADH dehydrogenase (ubiquinone) (non-electrogenic) activity), accepted elsewhere in this review. The NADH:ubiquinone reaction is the record's curated catalytic activity, assigned by NDH-2 family membership (InterPro IPR045024) and by similarity to characterised type II NADH dehydrogenases, not assayed for this S. pombe protein.

Summary: ORFeome YFP screen: mitochondrion.

Summary: PAINT inference of mitochondrial location.

Reason: The phylogenetic inference agrees with the experimental location of this protein, which UniProt records as mitochondrion from the genome-wide YFP localisation screen (PMID:16823372), and with the predicted N-terminal mitochondrial transit peptide (residues 1-37).

Summary: UniProt/ARBA location mapping; consistent.

Reason: This mapping derives from the record's SUBCELLULAR LOCATION comment, which rests on the experimental genome-wide localisation screen (PMID:16823372); the predicted mitochondrial transit peptide agrees.

Summary: Inner-membrane location by similarity to S. cerevisiae NDE1/NDI1.

Reason: The inner-membrane location is the S. cerevisiae ortholog's, transferred by similarity; it has not been resolved for this protein. The protein is experimentally mitochondrial, carries a predicted mitochondrial transit peptide, and belongs to the type II NADH dehydrogenase (NDH-2) family, whose mitochondrial members are monotopic enzymes on the inner membrane. The record has no TRANSMEM feature, consistent with peripheral rather than integral attachment. The quotes support the mitochondrial location and the family membership; the sub-compartment rests on the family argument.

Summary: Type II NADH dehydrogenases feed electrons from NADH to ubiquinone; S. pombe, like S. cerevisiae, lacks complex I.

Reason: The record assigns the NADH:ubiquinone reaction and NADH oxidation by similarity to characterised NDH-2 enzymes, and places the protein in mitochondria experimentally (PMID:16823372). Participation in respiratory electron transport is inferred from that activity and location, not shown for this protein.

Summary: Broad parent of the mitochondrial NADH-to-ubiquinone electron transport annotated in this review.

Reason: True but entailed by GO:0006120 (mitochondrial electron transport, NADH to ubiquinone), accepted elsewhere in this review. That process rests on the NADH:ubiquinone catalytic activity the record assigns by NDH-2 family similarity and on the experimental mitochondrial location from the S. pombe genome-wide localisation screen (PMID:16823372).

Summary: Generic oxidoreductase.

Reason: True but entailed by GO:0120555 (NADH dehydrogenase (ubiquinone) (non-electrogenic) activity), accepted elsewhere in this review. The NADH:ubiquinone reaction is the record's curated catalytic activity, assigned by NDH-2 family membership and similarity, not assayed in S. pombe.

Summary: Generic oxidoreductase.

Reason: True but entailed by GO:0120555 (NADH dehydrogenase (ubiquinone) (non-electrogenic) activity), accepted elsewhere in this review. The InterPro FAD/NAD-binding domain mapping is consistent with the record's curated NADH:ubiquinone catalytic activity, assigned by NDH-2 family membership and similarity, not assayed in S. pombe.

Summary: InterPro/EC mapping to the old term for non-electrogenic NADH:quinone oxidoreductase. GO:0120555 is the ubiquinone-specific child, which the oxphos module and the S. cerevisiae NDI1/NDE1 reviews use.

Reason: The record's curated catalytic activities give both the generic quinone reaction (EC 1.6.5.9, the basis of this mapping) and the ubiquinone-specific reaction (RHEA:23152), assigned by NDH-2 family membership and similarity to characterised type II NADH dehydrogenases rather than by an assay of this S. pombe protein. Ubiquinone is the mitochondrial substrate, so the more specific GO:0120555, already annotated to this gene, is the better term.

Proposed replacements: NADH dehydrogenase (ubiquinone) (non-electrogenic) activity

Summary: EC/RHEA mapping: type II (non-electrogenic) NADH:ubiquinone oxidoreductase.

Reason: RHEA:23152 is the record's curated catalytic activity; it rests on membership of the NDH-2 family (FAD and NAD binding regions annotated by similarity), not on an assay of this protein.