SPAC4H3.09 (UniProt Q10217; the fission yeast ACP1/NDUFAB1 ortholog) encodes the mitochondrial acyl carrier protein. Its 4'-phosphopantetheinylated serine carries acyl intermediates of mitochondrial type II fatty acid synthesis (mct1, oar2, htd2, etr1), producing octanoyl-ACP for lipoic acid synthesis. By orthology, acyl-ACP also binds Isd11 in the Nfs1-Isd11 cysteine desulfurase complex, coupling mtFAS to Fe-S cluster biogenesis. It is mitochondrial in a genome-wide screen.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000035 acyl binding | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Acyl binding via the phosphopantetheine arm; subsidiary to carrier activity. Reason: By similarity (UniProt ECO:0000250, no ortholog named) and ACP family membership (HAMAP MF_01217, phosphopantetheine attachment site): the acyl chain is held in thioester linkage on the 4-phosphopantetheine prosthetic group, so acyl binding is true. It is the mechanistic basis of the acyl carrier activity rather than a separate function, so non-core. No S. pombe experiment. Supporting Evidence: file:SCHPO/SPAC4H3.09/SPAC4H3.09-uniprot.txt fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group (By similarity). file:SCHPO/SPAC4H3.09/SPAC4H3.09-uniprot.txt Carrier of the growing fatty acid chain in fatty acid biosynthesis (By similarity). file:SCHPO/SPAC4H3.09/SPAC4H3.09-uniprot.txt Belongs to the acyl carrier protein (ACP) family. |
| GO:0000036 acyl carrier activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference: mitochondrial ACP carries acyl intermediates of mtFAS. Reason: Supported by the curated by-similarity UniProt function and PTM notes (carrier of the growing acyl chain, held as a thioester on the 4'-phosphopantetheine prosthetic group at Ser69) and by ACP family membership (HAMAP MF_01217, PANTHER PTHR20863:SF28); no S. pombe experiment is cited. Supporting Evidence: file:SCHPO/SPAC4H3.09/SPAC4H3.09-uniprot.txt Carrier of the growing fatty acid chain in fatty acid biosynthesis (By similarity). file:SCHPO/SPAC4H3.09/SPAC4H3.09-uniprot.txt fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group file:SCHPO/SPAC4H3.09/SPAC4H3.09-uniprot.txt Belongs to the acyl carrier protein (ACP) family. |
| GO:0005739 mitochondrion | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: ORFeome YFP screen: mitochondrion. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005739 mitochondrion | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference of mitochondrial location. Reason: Consistent with the curated by-similarity UniProt location and a predicted N-terminal mitochondrial transit peptide (residues 1-28), and with the HDA ORFeome localization of this gene. Supporting Evidence: file:SCHPO/SPAC4H3.09/SPAC4H3.09-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}. file:SCHPO/SPAC4H3.09/SPAC4H3.09-uniprot.txt TRANSIT 1..28 |
| GO:0005739 mitochondrion | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt/ARBA location mapping; consistent. Reason: The mapping rests on the curated by-similarity UniProt subcellular location (SL-0173), corroborated by the predicted mitochondrial transit peptide (residues 1-28). Supporting Evidence: file:SCHPO/SPAC4H3.09/SPAC4H3.09-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}. file:SCHPO/SPAC4H3.09/SPAC4H3.09-uniprot.txt TRANSIT 1..28 |
| GO:0005759 mitochondrial matrix | ISS GO_REF:0000024 | ACCEPT | Summary: Matrix location by similarity. Reason: Transfer from S. cerevisiae Acp1 (P32463). The record supports mitochondrial import (by-similarity location and a cleavable N-terminal transit peptide, residues 1-28) and has no transmembrane or signal-peptide FT features (the FT lines are only TRANSIT, CHAIN, DOMAIN and MOD_RES), consistent with a soluble matrix protein; the matrix sub-compartment itself rests on the ortholog and on mtFAS/ISC activities that occur in the matrix, not on an S. pombe assay. Supporting Evidence: file:SCHPO/SPAC4H3.09/SPAC4H3.09-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}. file:SCHPO/SPAC4H3.09/SPAC4H3.09-uniprot.txt TRANSIT 1..28 |
| GO:0006633 fatty acid biosynthetic process | IEA GO_REF:0000120 | ACCEPT | Summary: Mitochondrial type II fatty acid synthesis (octanoyl-ACP for lipoic acid). Reason: Supported by the curated by-similarity UniProt function (carrier of the growing fatty acid chain) and ACP family membership; the S. cerevisiae ortholog study places mitochondrial ACP in the type II fatty acid synthesis pathway. Supporting Evidence: file:SCHPO/SPAC4H3.09/SPAC4H3.09-uniprot.txt Carrier of the growing fatty acid chain in fatty acid biosynthesis (By similarity). file:SCHPO/SPAC4H3.09/SPAC4H3.09-uniprot.txt Belongs to the acyl carrier protein (ACP) family. PMID:27540631 The mitochondrial acyl carrier protein (ACP; Figure 1—figure supplement 1) plays a critical role in the evolutionarily conserved type II fatty acid biosynthesis pathway |
| GO:0006633 fatty acid biosynthetic process | ISS GO_REF:0000024 | ACCEPT | Summary: Mitochondrial type II fatty acid synthesis (octanoyl-ACP for lipoic acid). Reason: Transfer from S. cerevisiae Acp1 (P32463); supported by the curated by-similarity UniProt function note and by the S. cerevisiae study placing mitochondrial ACP in type II fatty acid synthesis. Supporting Evidence: file:SCHPO/SPAC4H3.09/SPAC4H3.09-uniprot.txt Carrier of the growing fatty acid chain in fatty acid biosynthesis (By similarity). PMID:27540631 The mitochondrial acyl carrier protein (ACP; Figure 1—figure supplement 1) plays a critical role in the evolutionarily conserved type II fatty acid biosynthesis pathway |
| GO:0044571 [2Fe-2S] cluster assembly | ISO PMID:27540631 The mitochondrial acyl carrier protein (ACP) coordinates mit... | ACCEPT | Summary: Transfer from S. cerevisiae Acp1: acyl-ACP binds Isd11 and supports Fe-S cluster biogenesis, coordinating mtFAS with ISC assembly. Supporting Evidence: PMID:27540631 The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty |
| GO:0099128 mitochondrial [2Fe-2S] assembly complex | IEA GO_REF:0000117 | ACCEPT | Summary: Mapping; acyl-ACP is part of the Nfs1-Isd11-ACP core complex. Reason: The ARBA mapping is not itself evidence; the claim rests on S. cerevisiae Acp1, shown by co-immunoprecipitation and BN-PAGE to be a stable subunit of the Nfs1-Isd11 (ISU) complex, and on the review describing ACP as a eukaryote-specific subunit of the NFS1 complex. No S. pombe assay; accepted by orthology. Supporting Evidence: PMID:27540631 Taken together these data demonstrate that Acp1 is a stable and evolutionarily conserved subunit of the ISU complex with Nfs1, Isd11, and Isu1. PMID:34660592 During this second step, additional eukaryotic-specific subunits (ISD11 and ACP) interact with NFS1 and form a stable complex. |
| GO:0099128 mitochondrial [2Fe-2S] assembly complex | TAS PMID:34660592 Biogenesis of Iron-Sulfur Clusters and Their Role in DNA Met... | ACCEPT | Summary: Review placing ACP in the mitochondrial [2Fe-2S] assembly complex. Supporting Evidence: PMID:34660592 Biogenesis of Iron-Sulfur Clusters and Their Role in DNA Metabolism |
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