SPAC694.03

Summary: PAINT inference (POF1 node): S. cerevisiae Pof1 is an NMN adenylyltransferase that works in NR/NMN salvage.

Reason: Swiss-Prot curates NMN adenylyltransferase activity (EC 2.7.7.1, RHEA:21360) for SPAC694.03 by similarity to S. cerevisiae Pof1 (UniProtKB:P25576), and places it in the eukaryotic NMN adenylyltransferase family, POF1 subfamily. This is ortholog and family evidence; no assay of the S. pombe protein is cached.

Summary: EC/RHEA mapping; consistent.

Reason: Swiss-Prot curates NMN adenylyltransferase activity (EC 2.7.7.1, RHEA:21360) for SPAC694.03 by similarity to S. cerevisiae Pof1 (UniProtKB:P25576), and places it in the eukaryotic NMN adenylyltransferase family, POF1 subfamily. This is ortholog and family evidence; no assay of the S. pombe protein is cached.

Summary: Curator transfer from S. cerevisiae POF1 (P25576).

Reason: Swiss-Prot curates NMN adenylyltransferase activity (EC 2.7.7.1, RHEA:21360) for SPAC694.03 by similarity to S. cerevisiae Pof1 (UniProtKB:P25576), and places it in the eukaryotic NMN adenylyltransferase family, POF1 subfamily. This is ortholog and family evidence; no assay of the S. pombe protein is cached.

Summary: ORFeome YFP screen recorded nuclear signal for SPAC694.03. Soluble metabolic enzymes often show diffuse nucleocytoplasmic signal in this screen; no nuclear role is known.

Reason: Experimental HDA kept, but not part of the core function.

Summary: PAINT inference; no nuclear role shown.

Reason: Not part of the core function.

Summary: UniProt location mapping of the nuclear screen signal; no nuclear role known.

Reason: Not part of the core function.

Summary: Phylogenetic inference of location; agrees with the localisation screen.

Summary: UniProt subcellular-location mapping (cytoplasm), derived from the localisation screen.

Summary: ORFeome YFP screen placed SPAC694.03 in the cytosol, consistent with a soluble metabolic enzyme.

Summary: Broad parent of the NAD+ salvage term accepted in this review.

Reason: True but uninformative. UniProt's by-similarity function (from S. cerevisiae Pof1, P25576) places the enzyme in NMN salvage to NAD(+), i.e. the accepted NAD+ biosynthetic process via the salvage pathway (GO:0034355), which entails nucleotide biosynthesis.

Summary: InterPro/UniPathway mapping; consistent.

Reason: Correct but general. Swiss-Prot places SPAC694.03 in the NMN-salvage branch of NAD+ synthesis by similarity to S. cerevisiae Pof1 (UniProtKB:P25576), so the specific descendant GO:0034355 NAD+ biosynthetic process via the salvage pathway, which the gene already carries (IBA), is the better term.

Proposed replacements: NAD+ biosynthetic process via the salvage pathway

Summary: Generic InterPro-derived parent of the specific activity, which is already annotated.

Reason: Correct but general. NadD/NMNAT family membership (IPR005248) and UniProt's by-similarity catalytic activity (from S. cerevisiae Pof1, P25576; EC 2.7.7.1) specify the nucleotidyltransferase as nicotinamide-nucleotide adenylyltransferase (GO:0000309), which the gene already carries. No S. pombe enzyme assay is cached.

Proposed replacements: nicotinamide-nucleotide adenylyltransferase activity

Summary: PAINT inference of ATP hydrolysis from the in vitro ATPase of S. cerevisiae Pof1, an NMN-specific NMNAT; in vitro activity of unknown physiological relevance.

Reason: The PAINT node is seeded by S. cerevisiae Pof1 (SGD:S000000552), whose purified protein showed ATPase activity in vitro (PMID:22204397). Pof1 was later shown to be an NMN-specific NMNAT (PMID:24759102), and nothing shows the ATPase is a physiological activity rather than a side reaction of the adenylyltransferase. Under the enzyme-specificity tiers this is real in vitro activity of unknown relevance, propagated into a species with no assay, so it is marked over-annotated rather than kept as a genuine secondary activity.

Summary: PAINT inference; Pof1-type NMNAT in NMN salvage.

Reason: Swiss-Prot assigns SPAC694.03 to NAD+ salvage via NMN by similarity to S. cerevisiae Pof1 (UniProtKB:P25576), which agrees with the PAINT node; this is ortholog evidence, not an S. pombe experiment.