SPBC1718.04 (UniProt Q9P7P0) encodes an ER glycerol-3-phosphate acyltransferase (GPAT; Gpt2/Sct1 family). It acylates glycerol 3-phosphate (and by similarity dihydroxyacetone phosphate) at sn-1, the first step of de novo phosphatidic acid synthesis for phospholipids and triacylglycerol. Function is assigned by orthology; it is in the ER in a genome-wide screen.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004366 glycerol-3-phosphate O-acyltransferase activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference: glycerol-3-phosphate acyltransferase (S. cerevisiae Gpt2/Sct1 node). Reason: No S. pombe biochemistry exists. The protein is classified in the PANTHER glycerol-3-phosphate O-acyltransferase family (PTHR31605) and the GPAT/DAPAT InterPro family, within the acyltransferase superfamily; the PAINT node rests on S. cerevisiae Gpt2/Sct1, characterised GPATs. Family membership supports the activity by orthology. Supporting Evidence: file:SCHPO/SPBC1718.04/SPBC1718.04-uniprot.txt Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. file:SCHPO/SPBC1718.04/SPBC1718.04-uniprot.txt PTHR31605; GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1 file:SCHPO/SPBC1718.04/SPBC1718.04-uniprot.txt IPR052744; GPAT/DAPAT |
| GO:0005783 endoplasmic reticulum | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: ORFeome screen: ER. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005783 endoplasmic reticulum | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference; consistent. Reason: UniProt records an endoplasmic reticulum membrane location with experimental evidence from the ORFeome screen (ECO:0000269|PubMed:16823372), and four predicted transmembrane helices (ECO:0000255); this supports the ER claim, with ER membrane as the sub-compartment. Supporting Evidence: file:SCHPO/SPBC1718.04/SPBC1718.04-uniprot.txt SUBCELLULAR LOCATION: Endoplasmic reticulum membrane file:SCHPO/SPBC1718.04/SPBC1718.04-uniprot.txt Multi-pass membrane protein |
| GO:0005789 endoplasmic reticulum membrane | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt mapping; consistent. Reason: UniProt records an endoplasmic reticulum membrane location with experimental evidence from the ORFeome screen (ECO:0000269|PubMed:16823372), and four predicted transmembrane helices (ECO:0000255); this supports the ER claim, with ER membrane as the sub-compartment. Supporting Evidence: file:SCHPO/SPBC1718.04/SPBC1718.04-uniprot.txt SUBCELLULAR LOCATION: Endoplasmic reticulum membrane file:SCHPO/SPBC1718.04/SPBC1718.04-uniprot.txt Multi-pass membrane protein |
| GO:0006654 phosphatidic acid biosynthetic process | TAS PMID:32930427 Metabolism of Storage Lipids and the Role of Lipid Droplets ... | ACCEPT | Summary: Review of S. pombe storage-lipid metabolism listing this GPAT in the first step of phosphatidic acid synthesis. Supporting Evidence: PMID:32930427 Storage lipids, triacylglycerols (TAG), and steryl esters (SE), are predominant constituents of lipid droplets (LD) in fungi. |
| GO:0008654 phospholipid biosynthetic process | IBA GO_REF:0000033 | TOO GENERAL | Summary: Broad parent of the phosphatidic acid biosynthetic process annotated in this review. Reason: True but entailed by GO:0006654 (phosphatidic acid biosynthetic process), accepted elsewhere in this review. The basis is family membership (PANTHER PTHR31605 glycerol-3-phosphate O-acyltransferase family; InterPro IPR052744 GPAT/DAPAT), shared with S. cerevisiae Gpt2/Sct1; there is no S. pombe experiment. Supporting Evidence: file:SCHPO/SPBC1718.04/SPBC1718.04-uniprot.txt PANTHER; PTHR31605; GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1; 1. file:SCHPO/SPBC1718.04/SPBC1718.04-uniprot.txt InterPro; IPR052744; GPAT/DAPAT. |
| GO:0016287 glycerone-phosphate O-acyltransferase activity | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: PAINT inference of DHAP acyltransferase activity: yeast Gpt2/Sct1 acylate both G3P and DHAP. Reason: Family membership only. The protein falls in the InterPro GPAT/DAPAT family (IPR052744) and the PANTHER glycerol-3-phosphate O-acyltransferase family (PTHR31605), whose characterised S. cerevisiae members Gpt2 and Sct1 acylate both glycerol 3-phosphate and dihydroxyacetone phosphate. DHAP acylation is plausible but untested in S. pombe and secondary to the G3P acyltransferase activity. Supporting Evidence: file:SCHPO/SPBC1718.04/SPBC1718.04-uniprot.txt PANTHER; PTHR31605; GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1; 1. file:SCHPO/SPBC1718.04/SPBC1718.04-uniprot.txt InterPro; IPR052744; GPAT/DAPAT. |
| GO:0016746 acyltransferase activity | IEA GO_REF:0000002 | MODIFY | Summary: Generic InterPro-derived parent of the specific activity, which is already annotated. Reason: Correct but general. Family membership (PANTHER PTHR31605 glycerol-3-phosphate O-acyltransferase family; InterPro IPR052744 GPAT/DAPAT) supports the specific G3P acyltransferase activity GO:0004366, already annotated to this gene; no S. pombe assay. The UniProt SIMILARITY line places it more loosely in the 1-acyl-sn-glycerol-3-phosphate acyltransferase superfamily. Proposed replacements: glycerol-3-phosphate O-acyltransferase activity Supporting Evidence: file:SCHPO/SPBC1718.04/SPBC1718.04-uniprot.txt PANTHER; PTHR31605; GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1; 1. file:SCHPO/SPBC1718.04/SPBC1718.04-uniprot.txt InterPro; IPR052744; GPAT/DAPAT. |
Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)