SPBC947.15c (UniProt O43090) encodes one of the fission yeast type II NADH dehydrogenases (NDH-2; EC 1.6.5.9), single-subunit flavoproteins that oxidise NADH and reduce ubiquinone without pumping protons. S. pombe has no complex I, so these enzymes are the entry point for NADH electrons into the respiratory chain. By similarity this paralog is matrix-facing (Ndi1-like). It is mitochondrial in a genome-wide screen.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003954 NADH dehydrogenase activity | IEA GO_REF:0000002 | TOO GENERAL | Summary: Generic NADH dehydrogenase; the specific non-electrogenic NADH:ubiquinone activity is annotated. Reason: The specific activity, NADH dehydrogenase (ubiquinone) (non-electrogenic) activity (GO:0120555), is asserted (ACCEPT) elsewhere in this review and entails this term. It rests on the by-similarity (ECO:0000250) NADH oxidation function, the curated ubiquinone reaction line and NDH-2 family membership (IPR045024); no S. pombe enzyme assay. Supporting Evidence: file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt Reaction=a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+) file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt Catalyzes the oxidation of NADH. file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt InterPro; IPR045024; NDH-2. |
| GO:0005739 mitochondrion | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: ORFeome YFP screen: mitochondrion. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005739 mitochondrion | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference of mitochondrial location. Reason: The phylogenetic inference agrees with this protein's own data. UniProt records a mitochondrial location from the S. pombe ORFeome localization screen (PubMed:16823372) and a predicted N-terminal mitochondrial transit peptide (residues 1-35). Supporting Evidence: file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt Mitochondrion {ECO:0000269|PubMed:16823372} file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt TRANSIT 1..35 |
| GO:0005739 mitochondrion | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt/ARBA location mapping; consistent. Reason: The mapped UniProt subcellular location rests on the S. pombe ORFeome localization screen (PubMed:16823372), and the sequence has a predicted mitochondrial transit peptide. Supporting Evidence: file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt Mitochondrion {ECO:0000269|PubMed:16823372} file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt TRANSIT 1..35 |
| GO:0005759 mitochondrial matrix | ISO GO_REF:0000024 | ACCEPT | Summary: Matrix-facing location by similarity to S. cerevisiae Ndi1. Reason: The matrix-side claim is the PomBase curator's orthology transfer from S. cerevisiae Ndi1 (the internal, matrix-facing NDH-2); there is no S. pombe sub-mitochondrial assay. This protein's own record supports only the organelle (ORFeome screen) and a predicted cleavable presequence, which is consistent with, but does not by itself establish, matrix-side topology, since external NDH-2s also carry presequences. Accepted in deference to the curator's ortholog assignment. Supporting Evidence: file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt TRANSIT 1..35 file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt Mitochondrion {ECO:0000269|PubMed:16823372} |
| GO:0006120 mitochondrial electron transport, NADH to ubiquinone | ISO GO_REF:0000024 | ACCEPT | Summary: Type II NADH dehydrogenases feed electrons from NADH to ubiquinone; S. pombe, like S. cerevisiae, lacks complex I. Reason: UniProt's by-similarity function statement and curated ubiquinone reaction line, with NDH-2 family membership, support NADH-to-ubiquinone electron transfer; the process assignment is transferred from S. cerevisiae Ndi1 and has not been tested in S. pombe. Supporting Evidence: file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt Catalyzes the oxidation of NADH. file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt Reaction=a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+) file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt InterPro; IPR045024; NDH-2. |
| GO:0016491 oxidoreductase activity | IBA GO_REF:0000033 | TOO GENERAL | Summary: Generic oxidoreductase. Reason: The specific activity, NADH dehydrogenase (ubiquinone) (non-electrogenic) activity (GO:0120555), is asserted (ACCEPT) elsewhere in this review and entails this term. It rests on the by-similarity (ECO:0000250) NADH oxidation function, the curated ubiquinone reaction line and NDH-2 family membership (IPR045024); no S. pombe enzyme assay. Supporting Evidence: file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt Reaction=a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+) file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt Catalyzes the oxidation of NADH. file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt InterPro; IPR045024; NDH-2. |
| GO:0016491 oxidoreductase activity | IEA GO_REF:0000002 | TOO GENERAL | Summary: Generic oxidoreductase. Reason: The specific activity, NADH dehydrogenase (ubiquinone) (non-electrogenic) activity (GO:0120555), is asserted (ACCEPT) elsewhere in this review and entails this term. It rests on the by-similarity (ECO:0000250) NADH oxidation function, the curated ubiquinone reaction line and NDH-2 family membership (IPR045024); no S. pombe enzyme assay. Supporting Evidence: file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt Reaction=a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+) file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt Catalyzes the oxidation of NADH. file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt InterPro; IPR045024; NDH-2. |
| GO:0050136 NADH dehydrogenase (quinone) (non-electrogenic) activity | IEA GO_REF:0000120 | MODIFY | Summary: InterPro/EC mapping to the old term for non-electrogenic NADH:quinone oxidoreductase. GO:0120555 is the ubiquinone-specific child, which the oxphos module and the S. cerevisiae NDI1/NDE1 reviews use. Reason: The essence (non-electrogenic NADH:quinone oxidoreductase) is sound: UniProt curates the quinone reaction (EC 1.6.5.9) and, more specifically, the ubiquinone reaction, with a by-similarity (ECO:0000250) NADH oxidation function and NDH-2 family membership (IPR045024). Mitochondrial NDH-2s reduce ubiquinone, so the ubiquinone-specific child GO:0120555, which the gene already carries, is the better term. No S. pombe enzyme assay. Proposed replacements: NADH dehydrogenase (ubiquinone) (non-electrogenic) activity Supporting Evidence: file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt Reaction=a quinone + NADH + H(+) = a quinol + NAD(+); file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt Reaction=a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+) file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt Catalyzes the oxidation of NADH. file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt InterPro; IPR045024; NDH-2. |
| GO:0120555 NADH dehydrogenase (ubiquinone) (non-electrogenic) activity | IEA GO_REF:0000116 | ACCEPT | Summary: EC/RHEA mapping: type II (non-electrogenic) NADH:ubiquinone oxidoreductase. Reason: RHEA:23152 is UniProt's curated ubiquinone reaction line; with the by-similarity NADH oxidation function and membership of the type II NADH dehydrogenase (NDH-2) family, whose members are single-subunit non-proton-pumping NADH:quinone oxidoreductases, this supports the non-electrogenic activity. No S. pombe enzyme assay exists. Supporting Evidence: file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt Reaction=a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+) file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt Catalyzes the oxidation of NADH. file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt Belongs to the NADH dehydrogenase family. file:SCHPO/SPBC947.15c/SPBC947.15c-uniprot.txt InterPro; IPR045024; NDH-2. |
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