SPCC1223.09 (uro1; UniProt O74409) encodes urate oxidase (uricase; EC 1.7.3.3), which oxidises uric acid to 5-hydroxyisourate, the first step of uric acid catabolism. This lets S. pombe use purines (hypoxanthine, xanthine, uric acid, allantoin) as sole nitrogen sources. uro1 mutants lack uricase activity. The protein is cytosolic in a genome-wide screen; whether it is also peroxisomal, as uricases are in many organisms, has not been tested.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004846 urate oxidase activity | EXP PMID:24177583 Genetic studies of purine breakdown in the fission yeast Sch... | ACCEPT | Summary: uro1 mutants lack uricase and cannot use uric acid as nitrogen source; SPCC1223.09 is the uro1 locus. Supporting Evidence: PMID:24177583 Mutants in the urol gene lack uricase, all1 lack allantoinase, ala1 lack allantoicase whilst in ure1, ure2, ure3 and ure4 genes lack urease activity. PMID:24177583 Purines such as hypoxanthine, xanthine, uric acid, allantoin and allantoic acid serve as sole nitrogen sources for the yeast Schizosaccharomyces pombe. |
| GO:0004846 urate oxidase activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference; consistent. Reason: UniProt's function and catalytic-activity statements carry experimental evidence (ECO:0000269|PubMed:4020341, not cached), and uro1 mutants lack uricase activity (PMID:24177583, the source of the EXP row); the IBA/IEA rows agree with this direct evidence. Supporting Evidence: file:SCHPO/SPCC1223.09/SPCC1223.09-uniprot.txt Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin. file:SCHPO/SPCC1223.09/SPCC1223.09-uniprot.txt Reaction=urate + O2 + H2O = 5-hydroxyisourate + H2O2 PMID:24177583 Mutants in the urol gene lack uricase |
| GO:0004846 urate oxidase activity | IEA GO_REF:0000120 | ACCEPT | Summary: EC/ARBA mapping; consistent. Reason: UniProt's function and catalytic-activity statements carry experimental evidence (ECO:0000269|PubMed:4020341, not cached), and uro1 mutants lack uricase activity (PMID:24177583, the source of the EXP row); the IBA/IEA rows agree with this direct evidence. Supporting Evidence: file:SCHPO/SPCC1223.09/SPCC1223.09-uniprot.txt Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin. file:SCHPO/SPCC1223.09/SPCC1223.09-uniprot.txt Reaction=urate + O2 + H2O = 5-hydroxyisourate + H2O2 PMID:24177583 Mutants in the urol gene lack uricase |
| GO:0005634 nucleus | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | KEEP AS NON CORE | Summary: ORFeome YFP screen recorded nuclear signal for SPCC1223.09. Soluble metabolic enzymes often show diffuse nucleocytoplasmic signal in this screen; no nuclear role is known. Reason: Experimental HDA kept, but not part of the core function. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005634 nucleus | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: UniProt location mapping of the nuclear screen signal; no nuclear role known. Reason: Not part of the core function. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt subcellular-location mapping (cytoplasm), derived from the localisation screen. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005777 peroxisome | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: PAINT inference of peroxisomal location (uricase is peroxisomal in many organisms). The ORFeome screen places the S. pombe protein in the cytosol, and fission yeast peroxisomal uricase has not been demonstrated. Reason: Not shown in S. pombe. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005777 peroxisome | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: UniProt mapping; as above. Reason: As above. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005829 cytosol | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: ORFeome YFP screen placed SPCC1223.09 in the cytosol, consistent with a soluble metabolic enzyme. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0006144 purine nucleobase metabolic process | IEA GO_REF:0000002 | TOO GENERAL | Summary: Broad parent. Reason: UniProt curates the urate oxidase function with experimental evidence (ECO:0000269, PubMed:4020341; paper not cached) and the InterPro uricase signature behind this row confirms family membership. The protein acts in purine nucleobase catabolic process, asserted elsewhere in this review, which entails this broad metabolism term. Supporting Evidence: file:SCHPO/SPCC1223.09/SPCC1223.09-uniprot.txt Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin. file:SCHPO/SPCC1223.09/SPCC1223.09-uniprot.txt Purine metabolism; urate degradation; (S)-allantoin from file:SCHPO/SPCC1223.09/SPCC1223.09-uniprot.txt InterPro; IPR019842; Uricase_CS. |
| GO:0006145 purine nucleobase catabolic process | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference; uricase is in the purine catabolic pathway. Supporting Evidence: PMID:24177583 Purines such as hypoxanthine, xanthine, uric acid, allantoin and allantoic acid serve as sole nitrogen sources for the yeast Schizosaccharomyces pombe. |
| GO:0019627 urea metabolic process | IC GO_REF:0000111 | KEEP AS NON CORE | Summary: Curator inference to urea metabolism; urate breakdown eventually releases urea, but uricase is several steps upstream. Supporting Evidence: PMID:24177583 Mutants in the urol gene lack uricase, all1 lack allantoinase, ala1 lack allantoicase whilst in ure1, ure2, ure3 and ure4 genes lack urease activity. |
| GO:0019628 urate catabolic process | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference; uricase catalyses the first step of urate catabolism. Reason: UniProt's experimentally supported function note (ECO:0000269|PubMed:4020341) places uricase at the first step of urate degradation to (S)-allantoin, and uro1 mutants cannot use uric acid as a nitrogen source (PMID:24177583). Supporting Evidence: file:SCHPO/SPCC1223.09/SPCC1223.09-uniprot.txt Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin. file:SCHPO/SPCC1223.09/SPCC1223.09-uniprot.txt Purine metabolism; urate degradation; (S)-allantoin from |
| GO:0019628 urate catabolic process | IEA GO_REF:0000041 | ACCEPT | Summary: UniPathway mapping; consistent. Reason: UniProt's experimentally supported function note (ECO:0000269|PubMed:4020341) places uricase at the first step of urate degradation to (S)-allantoin, and uro1 mutants cannot use uric acid as a nitrogen source (PMID:24177583). Supporting Evidence: file:SCHPO/SPCC1223.09/SPCC1223.09-uniprot.txt Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin. file:SCHPO/SPCC1223.09/SPCC1223.09-uniprot.txt Purine metabolism; urate degradation; (S)-allantoin from |
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