| Aspect | Finding for SPCC16C4.02c / O74447 | Evidence type | Confidence | Key citation(s) |
|---|---|---|---|---|
| Identity / aliases | Target verified in retrieved evidence as **SPCC16C4.02 / SPCC16C4.02c** from *Schizosaccharomyces pombe*; the 2013 paper uses both **SPCC16C4.02** and a likely typographical variant **Spcc16c4.20c** in a figure legend, but context indicates the same Ino80-associated ORF tested genetically (pqac-00000004, pqac-00000005) | Experimental genetic | High | Tay et al., 2013, *PLoS ONE*, DOI: https://doi.org/10.1371/journal.pone.0055041 (pqac-00000004, pqac-00000005) |
| Organism / strain | The user-specified target is from **Schizosaccharomyces pombe (strain 972 / ATCC 24843)**; retrieved papers study **fission yeast S. pombe** but do not restate the UniProt strain designation in the extracted passages (pqac-00000004, pqac-00000005) | Experimental genetic | Medium | Tay et al., 2013, https://doi.org/10.1371/journal.pone.0055041 (pqac-00000004, pqac-00000005) |
| Known / putative complex membership | **Directly listed as an Ino80 chromatin-remodeling complex subunit/member** in fission yeast: “Ino80 (Nht1, SPCC16C4.02, Iec1, Ies2, Iec3, Ies4, Ies6, Arp5, Arp8)” (pqac-00000004, pqac-00000005) | Experimental genetic | High | Tay et al., 2013, https://doi.org/10.1371/journal.pone.0055041 (pqac-00000004, pqac-00000005) |
| Functional inferences | Strictly from retrieved evidence, SPCC16C4.02c is **implicated in chromatin remodeling linked to doxorubicin resistance**, because mutants in Ino80 subunits behaved epistatically and were grouped with SAGA and homologous recombination factors in the same functional network (pqac-00000004, pqac-00000005). More specific biochemical activity for SPCC16C4.02c itself was **not directly shown** in the retrieved texts. | Experimental genetic | Medium | Tay et al., 2013, https://doi.org/10.1371/journal.pone.0055041 (pqac-00000004, pqac-00000005) |
| Phenotypes / assays | **Doxorubicin sensitivity genetic interaction assay**: single and double mutants involving **Iec1, Spcc16c4.02c, and Nht1** were tested by **ten-fold serial dilution spotting on DOXO plates**; double mutants showed **no cumulative/synthetic increase in DOXO sensitivity**, supporting action in the same complex/pathway (pqac-00000003, pqac-00000004, pqac-00000005, pqac-00000008) | Experimental genetic | High | Tay et al., 2013, https://doi.org/10.1371/journal.pone.0055041 (pqac-00000003, pqac-00000004, pqac-00000005, pqac-00000008) |
| Quantitative stats | **Direct SPCC16C4.02c-specific quantitative effect sizes were not present** in retrieved passages. Available quantitative details are assay-format only (**ten-fold serial dilutions**) and study-level conditions noting some mutants scored at **75 mg/ml** or **165 mg/ml DOXO**, but these concentrations were not explicitly assigned to SPCC16C4.02c in the extracted text (pqac-00000005). | Experimental genetic | Low | Tay et al., 2013, https://doi.org/10.1371/journal.pone.0055041 (pqac-00000005) |
| Relation to recent 2023–2024 work | Recent retrieved 2023 Ino80/quiescence work supports the broader importance of **Ino80 complex** in quiescent transcriptional control, H2A.Z eviction/relocalization, and survival in G0, but **SPCC16C4.02c was not explicitly mentioned in the extracted passages**; therefore this only strengthens the plausibility of an Ino80-related role, not a direct annotation for this ORF (pqac-00000006, pqac-00000007) | Transcriptomics | Low | Zahedi et al., 2023, *Chromosome Research*, DOI: https://doi.org/10.1007/s10577-023-09723-x (pqac-00000006, pqac-00000007) |
| Domain / family evidence | User-provided target metadata indicates **ARM-type fold / Neurochondrin-like domain (PF05536/IPR008709)**, but **no retrieved paper directly linked these domains to SPCC16C4.02c function in fission yeast**. One unrelated neurochondrin paper mentions palmitoylation-dependent targeting of metazoan neurochondrin to Rab5-positive endosomes, not the fungal ORF (pqac-00000002). | Computational/domain | Low | Gottlieb, 2015, neurochondrin mention only; no SPCC16C4.02c evidence (pqac-00000002) |
| Key citations with year and URL/DOI | **2013:** Tay et al., *Cellular Robustness Conferred by Genetic Crosstalk Underlies Resistance against Chemotherapeutic Drug Doxorubicin in Fission Yeast*, *PLoS ONE* 8:e55041, DOI/URL: https://doi.org/10.1371/journal.pone.0055041 — direct mention of SPCC16C4.02 as Ino80 component and genetic assay target. **2023:** Zahedi et al., *An essential role for the Ino80 chromatin remodeling complex in regulation of gene expression during cellular quiescence*, *Chromosome Research* 31(2), DOI/URL: https://doi.org/10.1007/s10577-023-09723-x — broader Ino80 context, no direct SPCC16C4.02c mention in extracted passages (pqac-00000004, pqac-00000005, pqac-00000006, pqac-00000007) | Experimental genetic; transcriptomics | High for 2013 direct mention / Low for 2023 indirect context | Tay et al., 2013; Zahedi et al., 2023 (pqac-00000004, pqac-00000005, pqac-00000006, pqac-00000007) |


*Table: This table summarizes the retrieved evidence for the fission yeast gene SPCC16C4.02c (UniProt O74447). It distinguishes direct gene-specific evidence from broader Ino80-complex context and indicates confidence based on whether SPCC16C4.02c was explicitly named.*