aco2 (mrpl49; SPBP4H10.15; UniProt Q9P7D4) encodes, through alternative polyadenylation, either a single aconitase-family domain or a fusion of that domain to the mitochondrial large-subunit ribosomal protein bL21m. The fusion is found in mitochondria, cytosol and nucleus. aco2 is essential, and its viability defect is complemented by the bL21m domain, which is needed for mitochondrial translation, not by the aconitase domain. By similarity to S. cerevisiae Aco2, a homocitrate dehydratase dedicated to lysine synthesis, the aconitase domain is assigned the cis-homoaconitate step of the alpha-aminoadipate pathway, but that role has not been tested in S. pombe.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003735 structural constituent of ribosome | ISO GO_REF:0000024 | ACCEPT | Summary: The C-terminal bL21m domain of the fusion is a mitoribosomal protein (transfer from S. cerevisiae MRPL49); it is the essential part of aco2. Supporting Evidence: PMID:25724335 The viability defect of aco2 mutation is complemented not by the aconitase domain but by the bL21 domain, which enables mitochondrial tran |
| GO:0003994 aconitate hydratase activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference of aconitase activity for the N-terminal aconitase domain. Supporting Evidence: PMID:25724335 Two types of aco2(+) transcripts are generated via alternative poly (A) site selection, producing both a single aconitase domain protein and the fusion form. |
| GO:0003994 aconitate hydratase activity | IEA GO_REF:0000002 | ACCEPT | Summary: InterPro mapping; consistent. Supporting Evidence: PMID:25724335 Two types of aco2(+) transcripts are generated via alternative poly (A) site selection, producing both a single aconitase domain protein and the fusion form. |
| GO:0005634 nucleus | IDA PMID:25724335 Essential function of Aco2, a fusion protein of aconitase an... | KEEP AS NON CORE | Summary: The fusion protein is also found in the nucleus; no nuclear role is defined. Reason: Not part of the core function. Supporting Evidence: PMID:25724335 The bL21-fused Aco2 protein resides in mitochondria as well as in the cytosol and the nucleus. |
| GO:0005634 nucleus | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: UniProt mapping; as above. Reason: Not part of the core function. Supporting Evidence: PMID:25724335 The bL21-fused Aco2 protein resides in mitochondria as well as in the cytosol and the nucleus. |
| GO:0005737 cytoplasm | IDA PMID:25724335 Essential function of Aco2, a fusion protein of aconitase an... | KEEP AS NON CORE | Summary: Cytosolic pool of the fusion protein; role unclear. Reason: Not part of the core function. Supporting Evidence: PMID:25724335 The bL21-fused Aco2 protein resides in mitochondria as well as in the cytosol and the nucleus. |
| GO:0005739 mitochondrion | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference; consistent. Supporting Evidence: PMID:25724335 The bL21-fused Aco2 protein resides in mitochondria as well as in the cytosol and the nucleus. |
| GO:0005739 mitochondrion | IDA PMID:25724335 Essential function of Aco2, a fusion protein of aconitase an... | ACCEPT | Summary: Aco2 resides in mitochondria. Supporting Evidence: PMID:25724335 The bL21-fused Aco2 protein resides in mitochondria as well as in the cytosol and the nucleus. |
| GO:0005739 mitochondrion | IEA GO_REF:0000120 | ACCEPT | Summary: UniProt/ARBA mapping; consistent. Supporting Evidence: PMID:25724335 The bL21-fused Aco2 protein resides in mitochondria as well as in the cytosol and the nucleus. |
| GO:0005762 mitochondrial large ribosomal subunit | ISO GO_REF:0000024 | ACCEPT | Summary: bL21m domain is part of the mitochondrial large ribosomal subunit (transfer). Supporting Evidence: PMID:25724335 The viability defect of aco2 mutation is complemented not by the aconitase domain but by the bL21 domain, which enables mitochondrial tran |
| GO:0005762 mitochondrial large ribosomal subunit | NAS PMID:18245278 Bot1p is required for mitochondrial translation, respiratory... | ACCEPT | Summary: Author statement; consistent. Supporting Evidence: PMID:18245278 Bot1p is required for mitochondrial translation |
| GO:0005829 cytosol | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: PAINT inference from S. cerevisiae ACO2/ACO1 cytosolic pools; Aco2 is partly cytosolic in S. pombe. Supporting Evidence: PMID:25724335 The bL21-fused Aco2 protein resides in mitochondria as well as in the cytosol and the nucleus. |
| GO:0005840 ribosome | IEA GO_REF:0000002 | ACCEPT | Summary: InterPro mapping of the ribosomal-protein domain. Supporting Evidence: PMID:25724335 The viability defect of aco2 mutation is complemented not by the aconitase domain but by the bL21 domain, which enables mitochondrial tran |
| GO:0006099 tricarboxylic acid cycle | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: InterPro mapping to the TCA cycle. S. pombe aco1 is the main TCA aconitase; Aco2 aconitase-domain activity is dispensable for viability. In S. cerevisiae, Aco2 is a homocitrate dehydratase in lysine synthesis. Supporting Evidence: PMID:25724335 The viability defect of aco2 mutation is complemented not by the aconitase domain but by the bL21 domain, which enables mitochondrial tran |
| GO:0032543 mitochondrial translation | IMP PMID:25724335 Essential function of Aco2, a fusion protein of aconitase an... | ACCEPT | Summary: The bL21 domain supports mitochondrial translation and viability. Supporting Evidence: PMID:25724335 The viability defect of aco2 mutation is complemented not by the aconitase domain but by the bL21 domain, which enables mitochondrial tran |
| GO:0032543 mitochondrial translation | NAS PMID:18245278 Bot1p is required for mitochondrial translation, respiratory... | ACCEPT | Summary: Author statement; consistent. Supporting Evidence: PMID:18245278 Bot1p is required for mitochondrial translation |
| GO:0051539 4 iron, 4 sulfur cluster binding | IBA GO_REF:0000033 | ACCEPT | Summary: The aconitase domain uses a [4Fe-4S] cluster (PAINT). Supporting Evidence: PMID:25724335 Two types of aco2(+) transcripts are generated via alternative poly (A) site selection, producing both a single aconitase domain protein and the fusion form. |
| GO:0051539 4 iron, 4 sulfur cluster binding | IEA GO_REF:0000002 | ACCEPT | Summary: InterPro mapping; consistent. Supporting Evidence: PMID:25724335 Two types of aco2(+) transcripts are generated via alternative poly (A) site selection, producing both a single aconitase domain protein and the fusion form. |
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