Cbh1 is a CENP-B-related sequence-specific DNA-binding protein of fission yeast. It binds centromeric K-type repeat DNA and ARS3002 sequences and associates with both centromeric and noncentromeric chromatin. Cbh1 overlaps functionally with the related factor Abp1 in maintaining chromosome segregation and normal cell growth; loss of both proteins produces severe growth and segregation defects. Cbh1 belongs to the fission-yeast CENP-B family that also participates in repetitive-element surveillance, including Tf2 retrotransposon silencing and nuclear organization.
Summary: Cbh1 binding to centromeric DNA and its in vivo association with K-repeat chromatin support this chromosomal/nuclear location.
Reason: Cbh1 binding to centromeric DNA and its in vivo association with K-repeat chromatin support this chromosomal/nuclear location. The conclusion is directly grounded in studies of the fission-yeast Cbh protein, not simply inferred from the mammalian CENP-B name.
Cbh protein specifically interacts in vitro with the K-type repeat DNA, which is essential for centromere function.
GO:0000779 condensed chromosome, centromeric region
IDA PMID:10733588 Fission yeast homologs of human CENP-B have redundant functi...
ACCEPT
Summary: Cbh1 binding to centromeric DNA and its in vivo association with K-repeat chromatin support this chromosomal/nuclear location.
Reason: Cbh1 binding to centromeric DNA and its in vivo association with K-repeat chromatin support this chromosomal/nuclear location. The conclusion is directly grounded in studies of the fission-yeast Cbh protein, not simply inferred from the mammalian CENP-B name.
Cbh protein specifically interacts in vitro with the K-type repeat DNA, which is essential for centromere function.
GO:0000779 condensed chromosome, centromeric region
IDA PMID:9237993 Purification and characterization of a CENP-B homologue prot...
ACCEPT
Summary: Cbh1 binding to centromeric DNA and its in vivo association with K-repeat chromatin support this chromosomal/nuclear location.
Reason: Cbh1 binding to centromeric DNA and its in vivo association with K-repeat chromatin support this chromosomal/nuclear location. The conclusion is directly grounded in studies of the fission-yeast Cbh protein, not simply inferred from the mammalian CENP-B name.
Summary: Cbh1 binding to centromeric DNA and its in vivo association with K-repeat chromatin support this chromosomal/nuclear location.
Reason: Cbh1 binding to centromeric DNA and its in vivo association with K-repeat chromatin support this chromosomal/nuclear location. The conclusion is directly grounded in studies of the fission-yeast Cbh protein, not simply inferred from the mammalian CENP-B name.
Summary: This broad binding term is biologically correct, but Cbh1 was purified and directly shown to bind centromeric K-type DNA.
Reason: This broad binding term is biologically correct, but Cbh1 was purified and directly shown to bind centromeric K-type DNA. Centromeric DNA binding is the experimentally supported molecular specificity.
Summary: This broad binding term is biologically correct, but Cbh1 was purified and directly shown to bind centromeric K-type DNA.
Reason: This broad binding term is biologically correct, but Cbh1 was purified and directly shown to bind centromeric K-type DNA. Centromeric DNA binding is the experimentally supported molecular specificity.
HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ...
ACCEPT
Summary: Cbh1 binding to centromeric DNA and its in vivo association with K-repeat chromatin support this chromosomal/nuclear location.
Reason: Cbh1 binding to centromeric DNA and its in vivo association with K-repeat chromatin support this chromosomal/nuclear location. The conclusion is directly grounded in studies of the fission-yeast Cbh protein, not simply inferred from the mammalian CENP-B name.
Summary: Cbh1 binding to centromeric DNA and its in vivo association with K-repeat chromatin support this chromosomal/nuclear location.
Reason: Cbh1 binding to centromeric DNA and its in vivo association with K-repeat chromatin support this chromosomal/nuclear location. The conclusion is directly grounded in studies of the fission-yeast Cbh protein, not simply inferred from the mammalian CENP-B name.
Summary: Cbh1 binding to centromeric DNA and its in vivo association with K-repeat chromatin support this chromosomal/nuclear location.
Reason: Cbh1 binding to centromeric DNA and its in vivo association with K-repeat chromatin support this chromosomal/nuclear location. The conclusion is directly grounded in studies of the fission-yeast Cbh protein, not simply inferred from the mammalian CENP-B name.
Summary: Redundant Cbh1/Abp1 functions in chromosome segregation are experimentally supported.
Reason: The double-deletion study directly connects Cbh1 and Abp1 to chromosome segregation; functional redundancy does not make the process unknown. Replace the ND biological-process root with chromosome segregation using the supporting study, preserving the original machine-sourced row.
IDA PMID:10733588 Fission yeast homologs of human CENP-B have redundant functi...
ACCEPT
Summary: Centromeric K-type repeat DNA binding is the directly measured biochemical property of Cbh1.
Reason: Centromeric K-type repeat DNA binding is the directly measured biochemical property of Cbh1. Purification and DNase I footprinting identified a sequence preference, and in vivo chromatin association corroborates the centromeric context.
IDA PMID:9237993 Purification and characterization of a CENP-B homologue prot...
ACCEPT
Summary: Centromeric K-type repeat DNA binding is the directly measured biochemical property of Cbh1.
Reason: Centromeric K-type repeat DNA binding is the directly measured biochemical property of Cbh1. Purification and DNase I footprinting identified a sequence preference, and in vivo chromatin association corroborates the centromeric context.
These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.
Review rationale: Purified fission-yeast Cbh1 directly binds centromeric K-type repeat DNA and was characterized by DNA-binding and footprinting assays. Its in vivo centromeric-chromatin association was independently examined. These target observations directly establish nucleic acid binding. The target already has the more precise experimental centromeric DNA-binding annotation GO:0019237, so the broad prediction is correct but less precise.
Supporting Evidence:
PMID:9237993: "Cbh protein specifically interacts in vitro with the K-type repeat DNA, which is essential for centromere function."
PMID:10733588: "In vivo, epitope-tagged Cbhp associated with centromeric K repeat chromatin, as well as with noncentromeric regions."