Cis4 is a CDF-family zinc transporter that forms a heteromeric complex with Zrg17 in the cis-Golgi membrane. It transfers zinc from the cytosol into the secretory pathway, particularly under zinc-limiting conditions, and thereby supports intracellular zinc balance and Golgi-dependent protein trafficking. Its compartment and low-zinc role differ from the ER zinc transporter Zhf1.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000139 Golgi membrane | IDA PMID:18199682 Cation diffusion facilitator Cis4 is implicated in Golgi mem... | MODIFY | Summary: Cis4 localizes to the cis-Golgi membrane. Reason: Target microscopy and the subsequent physiological study resolve the Golgi assignment to its cis compartment. Proposed replacements: Golgi cis cisterna membrane Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. |
| GO:0005385 zinc ion transmembrane transporter activity | EXP PMID:29529046 Zinc transporters belonging to the Cation Diffusion Facilita... | ACCEPT | Summary: Cis4 mediates zinc transport across an organelle membrane. Reason: Zinc-dependent mutant phenotypes, interaction with Zrg17 and cytosolic zinc-sensor measurements support zinc specificity. The experiment addresses Cis4 directly, providing independent grounding for the IBA/InterPro assignment. Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. PMID:29529046 deletion of cis4 or zrg17 leads to higher levels of zinc accumulating in the cytosol under conditions of zinc deficiency |
| GO:0005385 zinc ion transmembrane transporter activity | IBA GO_REF:0000033 | ACCEPT | Summary: Cis4 mediates zinc transport across an organelle membrane. Reason: Zinc-dependent mutant phenotypes, interaction with Zrg17 and cytosolic zinc-sensor measurements support zinc specificity. The experiment addresses Cis4 directly, providing independent grounding for the IBA/InterPro assignment. Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. PMID:29529046 deletion of cis4 or zrg17 leads to higher levels of zinc accumulating in the cytosol under conditions of zinc deficiency |
| GO:0005385 zinc ion transmembrane transporter activity | IEA GO_REF:0000002 | ACCEPT | Summary: Cis4 mediates zinc transport across an organelle membrane. Reason: Zinc-dependent mutant phenotypes, interaction with Zrg17 and cytosolic zinc-sensor measurements support zinc specificity. The experiment addresses Cis4 directly, providing independent grounding for the IBA/InterPro assignment. Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. PMID:29529046 deletion of cis4 or zrg17 leads to higher levels of zinc accumulating in the cytosol under conditions of zinc deficiency |
| GO:0005385 zinc ion transmembrane transporter activity | IMP PMID:18199682 Cation diffusion facilitator Cis4 is implicated in Golgi mem... | ACCEPT | Summary: Cis4 mediates zinc transport across an organelle membrane. Reason: Zinc-dependent mutant phenotypes, interaction with Zrg17 and cytosolic zinc-sensor measurements support zinc specificity. The experiment addresses Cis4 directly, providing independent grounding for the IBA/InterPro assignment. Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. PMID:29529046 deletion of cis4 or zrg17 leads to higher levels of zinc accumulating in the cytosol under conditions of zinc deficiency |
| GO:0005789 endoplasmic reticulum membrane | IEA GO_REF:0000044 | UNDECIDED | Summary: An additional ER membrane pool is not established by the available target experiments. Reason: The experimentally distinguished compartments are cis-Golgi for Cis4/Zrg17 and ER for Zhf1. The curated UniProt ER mapping is not itself refutation or confirmation of an additional Cis4 pool; retain uncertainty rather than asserting that Cis4 can never enter the ER. Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. |
| GO:0005794 Golgi apparatus | IBA GO_REF:0000033 | MODIFY | Summary: Cis4 is a cis-Golgi membrane transporter. Reason: Target-specific localization is more precise than the broader compartment label. Proposed replacements: Golgi cis cisterna membrane Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. |
| GO:0005794 Golgi apparatus | IEA GO_REF:0000044 | MODIFY | Summary: Cis4 is a cis-Golgi membrane transporter. Reason: Target-specific localization is more precise than the broader compartment label. Proposed replacements: Golgi cis cisterna membrane Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. |
| GO:0006812 monoatomic cation transport | IEA GO_REF:0000002 | MODIFY | Summary: Cis4 transports zinc from cytosol into the Golgi lumen. Reason: The target studies establish substrate and transport direction; generic cation/organelle/transmembrane transport can be made specific. Proposed replacements: zinc ion import into Golgi lumen Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. |
| GO:0006829 zinc ion transport | IEA GO_REF:0000002 | MODIFY | Summary: Cis4 transports zinc from cytosol into the Golgi lumen. Reason: The target studies establish substrate and transport direction; generic cation/organelle/transmembrane transport can be made specific. Proposed replacements: zinc ion import into Golgi lumen Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. |
| GO:0006882 intracellular zinc ion homeostasis | IBA GO_REF:0000033 | ACCEPT | Summary: Cis4 maintains intracellular zinc homeostasis. Reason: Loss of Cis4 elevates cytosolic zinc under zinc limitation, demonstrating a homeostatic role through organellar import. Supporting Evidence: PMID:29529046 deletion of cis4 or zrg17 leads to higher levels of zinc accumulating in the cytosol under conditions of zinc deficiency |
| GO:0006882 intracellular zinc ion homeostasis | IDA PMID:29529046 Zinc transporters belonging to the Cation Diffusion Facilita... | ACCEPT | Summary: Cis4 maintains intracellular zinc homeostasis. Reason: Loss of Cis4 elevates cytosolic zinc under zinc limitation, demonstrating a homeostatic role through organellar import. Supporting Evidence: PMID:29529046 deletion of cis4 or zrg17 leads to higher levels of zinc accumulating in the cytosol under conditions of zinc deficiency |
| GO:0006882 intracellular zinc ion homeostasis | IEA GO_REF:0000002 | ACCEPT | Summary: Cis4 maintains intracellular zinc homeostasis. Reason: Loss of Cis4 elevates cytosolic zinc under zinc limitation, demonstrating a homeostatic role through organellar import. Supporting Evidence: PMID:29529046 deletion of cis4 or zrg17 leads to higher levels of zinc accumulating in the cytosol under conditions of zinc deficiency |
| GO:0006882 intracellular zinc ion homeostasis | IMP PMID:18199682 Cation diffusion facilitator Cis4 is implicated in Golgi mem... | ACCEPT | Summary: Cis4 maintains intracellular zinc homeostasis. Reason: Loss of Cis4 elevates cytosolic zinc under zinc limitation, demonstrating a homeostatic role through organellar import. Supporting Evidence: PMID:29529046 deletion of cis4 or zrg17 leads to higher levels of zinc accumulating in the cytosol under conditions of zinc deficiency |
| GO:0008324 monoatomic cation transmembrane transporter activity | IEA GO_REF:0000002 | MODIFY | Summary: The relevant cation is zinc. Reason: Target zinc-sensor measurements and zinc-responsive mutant phenotypes resolve the transporter substrate. Proposed replacements: zinc ion transmembrane transporter activity Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. PMID:29529046 deletion of cis4 or zrg17 leads to higher levels of zinc accumulating in the cytosol under conditions of zinc deficiency |
| GO:0016020 membrane | IEA GO_REF:0000002 | MODIFY | Summary: Cis4 is a cis-Golgi membrane transporter. Reason: Target-specific localization is more precise than the broader compartment label. Proposed replacements: Golgi cis cisterna membrane Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. |
| GO:0030003 intracellular monoatomic cation homeostasis | IEA GO_REF:0000117 | MODIFY | Summary: Cis4 contributes specifically to intracellular zinc homeostasis. Reason: The experimentally demonstrated ion and process are more precise than broad ion-homeostasis categories. Proposed replacements: intracellular zinc ion homeostasis Supporting Evidence: PMID:29529046 deletion of cis4 or zrg17 leads to higher levels of zinc accumulating in the cytosol under conditions of zinc deficiency |
| GO:0031410 cytoplasmic vesicle | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: A vesicular localization is compatible with secretory-pathway traffic but is not the established principal site. Reason: Retain the phylogenetic compartment inference as non-core; the direct target evidence establishes cis-Golgi transport and does not exclude a trafficking pool. Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. |
| GO:0055085 transmembrane transport | IEA GO_REF:0000002 | MODIFY | Summary: Cis4 transports zinc from cytosol into the Golgi lumen. Reason: The target studies establish substrate and transport direction; generic cation/organelle/transmembrane transport can be made specific. Proposed replacements: zinc ion import into Golgi lumen Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. |
| GO:0062111 zinc ion import into organelle | IDA PMID:29529046 Zinc transporters belonging to the Cation Diffusion Facilita... | MODIFY | Summary: Cis4 transports zinc from cytosol into the Golgi lumen. Reason: The target studies establish substrate and transport direction; generic cation/organelle/transmembrane transport can be made specific. Proposed replacements: zinc ion import into Golgi lumen Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. |
| GO:0098771 inorganic ion homeostasis | IEA GO_REF:0000117 | MODIFY | Summary: Cis4 contributes specifically to intracellular zinc homeostasis. Reason: The experimentally demonstrated ion and process are more precise than broad ion-homeostasis categories. Proposed replacements: intracellular zinc ion homeostasis Supporting Evidence: PMID:29529046 deletion of cis4 or zrg17 leads to higher levels of zinc accumulating in the cytosol under conditions of zinc deficiency |
| GO:1904257 zinc ion import into Golgi lumen | IBA GO_REF:0000033 | ACCEPT | Summary: Cis4 imports zinc into the Golgi lumen. Reason: The cis-Golgi localization, Zrg17 complex and mutant zinc-homeostasis data jointly establish the physiological direction and destination. Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. PMID:29529046 deletion of cis4 or zrg17 leads to higher levels of zinc accumulating in the cytosol under conditions of zinc deficiency |
| GO:1904257 zinc ion import into Golgi lumen | IMP PMID:18199682 Cation diffusion facilitator Cis4 is implicated in Golgi mem... | ACCEPT | Summary: Cis4 imports zinc into the Golgi lumen. Reason: The cis-Golgi localization, Zrg17 complex and mutant zinc-homeostasis data jointly establish the physiological direction and destination. Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. PMID:29529046 deletion of cis4 or zrg17 leads to higher levels of zinc accumulating in the cytosol under conditions of zinc deficiency |
| GO:1990674 Golgi cis cisterna membrane | IBA GO_REF:0000033 | ACCEPT | Summary: Cis4 acts in the cis-Golgi membrane. Reason: The target localization agrees with the phylogenetic inference; this is not a generic CDF-family compartment transfer. Supporting Evidence: PMID:29529046 Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. |
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