crt10

UniProt ID: O42996
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Review Status: COMPLETE
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Gene Description

Crt10 is a WD-repeat protein of the Crt10 family found in the nucleus and cytoplasm of fission yeast. Its conserved role is inferred to be surveillance and disposal of nonfunctional large-subunit ribosomal RNA. In budding yeast, Crt10 directs an Rtt101-Mms1 cullin ubiquitin ligase toward defective ribosomes; the equivalent protein interactions and molecular activity of fission-yeast Crt10 remain incompletely characterized.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0003674 molecular_function
ND
GO_REF:0000015 		ACCEPT
Summary: The ND molecular-function root correctly records that a specific molecular activity has not been established for fission-yeast Crt10.
Reason: The ND molecular-function root correctly records that a specific molecular activity has not been established for fission-yeast Crt10. Its Crt10/WD-repeat architecture supports an interaction protein, but does not identify a catalytic activity or a particular cullin partner.
GO:0005634 nucleus
HDA
PMID:16823372
ORFeome cloning and global analysis of protein localization ... 		ACCEPT
Summary: Nuclear and cytoplasmic localization is supported by the protein-localization study cited in the reviewed UniProt record (PMID:16823372); this is consistent with a nucleo-cytoplasmic RNA-surveillance protein.
Reason: Nuclear and cytoplasmic localization is supported by the protein-localization study cited in the reviewed UniProt record (PMID:16823372); this is consistent with a nucleo-cytoplasmic RNA-surveillance protein. Retain the compartment at the stated resolution.
Supporting Evidence:
file:SCHPO/crt10/crt10-uniprot.txt
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus CC {ECO:0000269|PubMed:16823372}.
GO:0005634 nucleus
IEA
GO_REF:0000044 		ACCEPT
Summary: Nuclear and cytoplasmic localization is supported by the protein-localization study cited in the reviewed UniProt record (PMID:16823372); this is consistent with a nucleo-cytoplasmic RNA-surveillance protein.
Reason: Nuclear and cytoplasmic localization is supported by the protein-localization study cited in the reviewed UniProt record (PMID:16823372); this is consistent with a nucleo-cytoplasmic RNA-surveillance protein. Retain the compartment at the stated resolution.
Supporting Evidence:
file:SCHPO/crt10/crt10-uniprot.txt
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus CC {ECO:0000269|PubMed:16823372}.
GO:0005737 cytoplasm
HDA
PMID:22146723
Systematic localization study on novel proteins encoded by m... 		UNDECIDED
Summary: The cited mitotic localization survey reports diverse cytoplasmic patterns among meiotically induced ORFs, but the accessible abstract does not identify the SPBC27B12.05 image or row.
Reason: The cited mitotic localization survey reports diverse cytoplasmic patterns among meiotically induced ORFs, but the accessible abstract does not identify the SPBC27B12.05 image or row. Cytoplasm is independently supported by PMID:16823372; retain this source-specific HDA assertion as unresolved until its target row is inspected.
GO:0005737 cytoplasm
IEA
GO_REF:0000044 		ACCEPT
Summary: Nuclear and cytoplasmic localization is supported by the protein-localization study cited in the reviewed UniProt record (PMID:16823372); this is consistent with a nucleo-cytoplasmic RNA-surveillance protein.
Reason: Nuclear and cytoplasmic localization is supported by the protein-localization study cited in the reviewed UniProt record (PMID:16823372); this is consistent with a nucleo-cytoplasmic RNA-surveillance protein. Retain the compartment at the stated resolution.
Supporting Evidence:
file:SCHPO/crt10/crt10-uniprot.txt
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus CC {ECO:0000269|PubMed:16823372}.
GO:0005829 cytosol
HDA
PMID:16823372
ORFeome cloning and global analysis of protein localization ... 		ACCEPT
Summary: Nuclear and cytoplasmic localization is supported by the protein-localization study cited in the reviewed UniProt record (PMID:16823372); this is consistent with a nucleo-cytoplasmic RNA-surveillance protein.
Reason: Nuclear and cytoplasmic localization is supported by the protein-localization study cited in the reviewed UniProt record (PMID:16823372); this is consistent with a nucleo-cytoplasmic RNA-surveillance protein. Retain the compartment at the stated resolution.
Supporting Evidence:
file:SCHPO/crt10/crt10-uniprot.txt
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus CC {ECO:0000269|PubMed:16823372}.
GO:0070651 nonfunctional rRNA decay
ISO
GO_REF:0000024 		ACCEPT
Summary: PomBase infers nonfunctional rRNA decay by curated orthology.
Reason: PomBase infers nonfunctional rRNA decay by curated orthology. The target has the diagnostic Crt10 family domain, and experiments in budding yeast show Crt10 is required for nonfunctional 25S rRNA decay. This supports the conserved pathway inference without assigning the target to a particular E3 complex.
Supporting Evidence:
PMID:25534857
We herein demonstrated that another accessory component, Crt10 was required for 25S NRD, but not for DNA repair, suggesting that this accessory component specifies the function of the E3 complex differently.
file:SCHPO/crt10/crt10-uniprot.txt
DR Pfam; PF08728; CRT10; 1.

Core Functions

Conserved Crt10-family factor inferred to participate in nonfunctional large-subunit rRNA decay. The exact molecular activity and cullin-binding partners in fission yeast are unresolved.

Directly Involved In:
nonfunctional rRNA decay
Cellular Locations:
cytosol nucleus
Supporting Evidence:
  • PMID:25534857
    We herein demonstrated that another accessory component, Crt10 was required for 25S NRD, but not for DNA repair, suggesting that this accessory component specifies the function of the E3 complex differently.

References

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Suggested Questions for Experts

Q: Does fission-yeast Crt10 bind Pcu4/Ddb1 or another cullin complex during nonfunctional rRNA decay, and does that association change after UV-B exposure?

External Prediction Reviews

These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.

ProtNLM2 External predictions

View prediction review YAML · crt10-protnlm-predictions-review.yaml · Review status: COMPLETE

Crt10 family biology supports nonfunctional rRNA decay, but the exact Cul4-complex and UV-B claims remain unresolved.

Source documents: genes/SCHPO/crt10/crt10-protnlm-source.xml · genes/SCHPO/crt10/crt10-uniprot-source.json · genes/SCHPO/crt10/crt10-uniprot.txt

Review score: 2 = concordant with evidence; 1 = uncertain; 0 = discordant with evidence. This is an assessment score, not a model probability.

GO:0080008 Cul4-RING E3 ubiquitin ligase complex GO_CC
UNC — Uncertain Review score: 1/2
Prediction method: ProtNLM2 · Version: pre-release post-processed-2026_02_28k.xml · file:SCHPO/crt10/crt10-protnlm-source.xml
Review rationale: Crt10 has a diagnostic CRT10 domain and a documented orthologous role in Rtt101-Mms1-dependent nonfunctional rRNA decay. This supports a relationship to cullin-mediated ubiquitination, but does not establish residence in the specific Cul4-RING E3 complex in fission yeast. The XML claim is a low-scoring phmmer transfer from Arabidopsis WDR5B Q9SY00 (model_score 0.11; phmmer_score 66.0); shared WD-repeat architecture does not demonstrate the relevant cullin partner or conserved subfamily function. Direct target-complex evidence or defensible orthology connecting this complex membership is missing. The claim is neither validated nor conclusively refuted.
Supporting Evidence:
  • PMID:25534857: "We herein demonstrated that another accessory component, Crt10 was required for 25S NRD, but not for DNA repair, suggesting that this accessory component specifies the function of the E3 complex differently."
  • file:SCHPO/crt10/crt10-uniprot.txt: "DR InterPro; IPR014839; Crt10."
GO:0071493 cellular response to UV-B GO_BP
UNC — Uncertain Review score: 1/2
Prediction method: ProtNLM2 · Version: pre-release post-processed-2026_02_28k.xml · file:SCHPO/crt10/crt10-protnlm-source.xml
Review rationale: The exact UV-B response prediction is not supported by the reviewed target annotations. Its source is a TMalign comparison to Arabidopsis DHU1 Q8GYY7 (model_score 0.11; chain-normalized scores 0.56569 and 0.36277), rather than a demonstrated conserved UV-B response mechanism. The experimentally characterized budding-yeast Crt10 directs nonfunctional 25S rRNA decay and is dispensable for the DNA-repair assay examined in that study, which does not exclude every possible UV-B response. Shared WD-repeat structure alone cannot validate this wavelength-specific phenotype in fission yeast. Target UV-B experiments or a conserved mechanistic link are missing, so UNC is appropriate.
Supporting Evidence:
  • PMID:25534857: "We herein demonstrated that another accessory component, Crt10 was required for 25S NRD, but not for DNA repair, suggesting that this accessory component specifies the function of the E3 complex differently."
  • file:SCHPO/crt10/crt10-uniprot.txt: "DR Pfam; PF08728; CRT10; 1. DR Pfam; PF00400; WD40; 1."

Deep Research

Falcon

(crt10-deep-research-falcon.md)

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📚 Additional Documentation

Notes

(crt10-notes.md)

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📄 View Raw YAML

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