id: O42996
gene_symbol: crt10
taxon:
  id: NCBITaxon:284812
  label: Schizosaccharomyces pombe (strain 972 / ATCC 24843)
status: COMPLETE
description: Crt10 family biology supports nonfunctional rRNA decay, but the exact Cul4-complex and UV-B
  claims remain unresolved.
source_documents:
- genes/SCHPO/crt10/crt10-protnlm-source.xml
- genes/SCHPO/crt10/crt10-uniprot-source.json
- genes/SCHPO/crt10/crt10-uniprot.txt
predictions:
- source_method: ProtNLM2
  source_version: pre-release post-processed-2026_02_28k.xml
  source_reference_id: file:SCHPO/crt10/crt10-protnlm-source.xml
  predicted_term:
    id: GO:0080008
    label: Cul4-RING E3 ubiquitin ligase complex
  predicted_term_type: GO_CC
  review:
    assessment: UNC
    confidence_score: 1
    summary: Crt10 has a diagnostic CRT10 domain and a documented orthologous role in Rtt101-Mms1-dependent
      nonfunctional rRNA decay. This supports a relationship to cullin-mediated ubiquitination, but does
      not establish residence in the specific Cul4-RING E3 complex in fission yeast. The XML claim is
      a low-scoring phmmer transfer from Arabidopsis WDR5B Q9SY00 (model_score 0.11; phmmer_score 66.0);
      shared WD-repeat architecture does not demonstrate the relevant cullin partner or conserved subfamily
      function. Direct target-complex evidence or defensible orthology connecting this complex membership
      is missing. The claim is neither validated nor conclusively refuted.
    supported_by:
    - &id001
      reference_id: PMID:25534857
      supporting_text: 'We herein demonstrated that another accessory component, Crt10

        was required for 25S NRD, but not for DNA repair, suggesting that this accessory

        component specifies the function of the E3 complex differently.'
    - reference_id: file:SCHPO/crt10/crt10-uniprot.txt
      supporting_text: DR   InterPro; IPR014839; Crt10.
- source_method: ProtNLM2
  source_version: pre-release post-processed-2026_02_28k.xml
  source_reference_id: file:SCHPO/crt10/crt10-protnlm-source.xml
  predicted_term:
    id: GO:0071493
    label: cellular response to UV-B
  predicted_term_type: GO_BP
  review:
    assessment: UNC
    confidence_score: 1
    summary: The exact UV-B response prediction is not supported by the reviewed target annotations. Its
      source is a TMalign comparison to Arabidopsis DHU1 Q8GYY7 (model_score 0.11; chain-normalized scores
      0.56569 and 0.36277), rather than a demonstrated conserved UV-B response mechanism. The experimentally
      characterized budding-yeast Crt10 directs nonfunctional 25S rRNA decay and is dispensable for the
      DNA-repair assay examined in that study, which does not exclude every possible UV-B response. Shared
      WD-repeat structure alone cannot validate this wavelength-specific phenotype in fission yeast. Target
      UV-B experiments or a conserved mechanistic link are missing, so UNC is appropriate.
    supported_by:
    - *id001
    - reference_id: file:SCHPO/crt10/crt10-uniprot.txt
      supporting_text: 'DR   Pfam; PF08728; CRT10; 1.

        DR   Pfam; PF00400; WD40; 1.'
references:
- id: PMID:25534857
  title: Crt10 directs the cullin-E3 ligase Rtt101 to nonfunctional 25S rRNA decay.
  findings: []
  full_text_unavailable: true
- id: file:SCHPO/crt10/crt10-uniprot.txt
  title: Frozen source record used for prediction assessment
  findings: []