erg8 (SPAC343.01c) encodes the fission-yeast GHMP-family phosphomevalonate kinase. The enzyme phosphorylates (R)-mevalonate 5-phosphate with ATP to form (R)-mevalonate 5-diphosphate, the second of three mevalonate-pathway reactions that produce isopentenyl diphosphate and feed fungal sterol biosynthesis. The protein is cytosolic by PomBase curation from the genome-wide S. pombe ORFeome localization screen, and the same screen also reported nuclear and mitochondrial signal for the tagged protein.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004631 phosphomevalonate kinase activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT infers phosphomevalonate kinase activity from PTN001274235, the GHMP-type phosphomevalonate kinase node seeded by Arabidopsis PMK and S. cerevisiae ERG8. S. pombe erg8 is a PTHR31814:SF2 family member and UniProt carries the expected Rhea reaction, so this core MF propagates correctly. Supporting Evidence: file:SCHPO/erg8/erg8-uniprot.txt Reaction=(R)-5-phosphomevalonate + ATP = (R)-5-diphosphomevalonate + ADP; Xref=Rhea:RHEA:16341 file:SCHPO/erg8/erg8-uniprot.txt PANTHER; PTHR31814:SF2; PHOSPHOMEVALONATE KINASE; 1. |
| GO:0004631 phosphomevalonate kinase activity | IEA GO_REF:0000120 | ACCEPT | Summary: Combined automated EC/Rhea/InterPro mapping to the same PMK reaction. Correct. Supporting Evidence: file:SCHPO/erg8/erg8-uniprot.txt Reaction=(R)-5-phosphomevalonate + ATP = (R)-5-diphosphomevalonate + ADP; Xref=Rhea:RHEA:16341 file:SCHPO/erg8/erg8-uniprot.txt InterPro; IPR016005; Erg8. |
| GO:0004631 phosphomevalonate kinase activity | ISS GO_REF:0000024 | ACCEPT | Summary: Curator transfer from S. cerevisiae ERG8 to its S. pombe ortholog. The transfer is consistent with the PAINT placement and with the UniProt catalytic reaction on Q9UT88. Supporting Evidence: file:SCHPO/erg8/erg8-uniprot.txt Reaction=(R)-5-phosphomevalonate + ATP = (R)-5-diphosphomevalonate + ADP; Xref=Rhea:RHEA:16341 |
| GO:0005634 nucleus | EXP PMID:16823372 ORFeome cloning and global analysis of protein localization ... | KEEP AS NON CORE | Summary: The ORFeome YFP screen reported nuclear erg8 signal. No nuclear phosphomevalonate-kinase role is known, but this EXP localization should be retained as a non-core high-throughput observation. Reason: High-throughput localization with no known nuclear function. Supporting Evidence: PMID:16823372 Next, we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein. |
| GO:0005634 nucleus | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: UniProt SubCell mapping of the same nuclear localization. Keep as non-core. Reason: High-throughput localization with no known nuclear function. Supporting Evidence: file:SCHPO/erg8/erg8-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Mitochondrion {ECO:0000269|PubMed:16823372}. Nucleus {ECO:0000269|PubMed:16823372}. |
| GO:0005737 cytoplasm | EXP PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: Cytoplasmic localization from the ORFeome YFP screen. Correct for a soluble PMK. Supporting Evidence: PMID:16823372 Next, we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein. |
| GO:0005737 cytoplasm | IEA GO_REF:0000120 | ACCEPT | Summary: UniProt SubCell mapping of the experimentally reported cytoplasmic localization. Correct. Supporting Evidence: file:SCHPO/erg8/erg8-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Mitochondrion {ECO:0000269|PubMed:16823372}. Nucleus {ECO:0000269|PubMed:16823372}. |
| GO:0005739 mitochondrion | EXP PMID:16823372 ORFeome cloning and global analysis of protein localization ... | UNDECIDED | Summary: The ORFeome YFP screen also recorded mitochondrial signal. Fungal Erg8 is expected to catalyze a cytosolic mevalonate-pathway step, and the cached Matsuyama et al. record is abstract-only, so the screen call cannot be adjudicated further here. Reason: The mitochondrial call comes from the same abstract-only high-throughput localization screen as the accepted cytosol and cytoplasm calls, but mitochondrial localization conflicts with the conserved cytosolic mevalonate-pathway role and cannot be resolved from the cached evidence. Supporting Evidence: PMID:16823372 Next, we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein. |
| GO:0005739 mitochondrion | IEA GO_REF:0000044 | UNDECIDED | Summary: UniProt SubCell mapping of the mitochondrial localization from the ORFeome screen. The underlying high-throughput observation cannot be checked from the cached abstract. Reason: The mitochondrial UniProtKB-SubCell mapping traces to the same abstract-only high-throughput screen as the accepted cytosol and cytoplasm calls, but mitochondrial localization conflicts with the conserved cytosolic mevalonate-pathway role and cannot be resolved from the cached evidence. Supporting Evidence: file:SCHPO/erg8/erg8-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Mitochondrion {ECO:0000269|PubMed:16823372}. Nucleus {ECO:0000269|PubMed:16823372}. |
| GO:0005777 peroxisome | IBA GO_REF:0000033 | REMOVE | Summary: The peroxisome IBA comes from PTN000781523, a plant PMK node seeded by Arabidopsis AT1G31910. PTHR31814 fungal members inherit the PMK activity from PTN001274235, but the peroxisome placement is a plant-lineage localization that should not propagate to S. pombe erg8. Reason: The PANTHER family review marks PTN000781523/GO:0005777 as NEEDS_PRUNING: fungal Erg8 catalyzes a cytosolic mevalonate-pathway step, while the peroxisomal placement is supported for the plant PMK seed and not for the fungal ERG8 clade. Propagation Review Root cause: PROPAGATION BAD Failure modes: COMPARTMENT OR COMPLEX MISMATCH LINEAGE OR TAXON MISMATCH Sources checked: PANTHER:PTN000781523 · PTN000781523 SUPPORTS SOURCE BUT NOT TARGET Plant peroxisomal PMK is not the fungal cytosolic ERG8 localization state. Supporting Evidence: file:SCHPO/erg8/erg8-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Mitochondrion {ECO:0000269|PubMed:16823372}. Nucleus {ECO:0000269|PubMed:16823372}. file:SCHPO/erg8/erg8-uniprot.txt PANTHER; PTHR31814:SF2; PHOSPHOMEVALONATE KINASE; 1. |
| GO:0005829 cytosol | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: PomBase curates cytosolic localization from the ORFeome YFP screen; this agrees with the expected soluble mevalonate-pathway PMK activity. Supporting Evidence: PMID:16823372 Next, we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein. |
| GO:0006696 ergosterol biosynthetic process | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT infers the fungal ergosterol-biosynthesis process from PTN001274236, a fungal node seeded by S. cerevisiae ERG8. erg8 provides the PMK step in the upstream mevalonate pathway that supplies isopentenyl diphosphate for sterol biosynthesis. Supporting Evidence: file:SCHPO/erg8/erg8-uniprot.txt Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 2/3. file:SCHPO/erg8/erg8-uniprot.txt Reaction=(R)-5-phosphomevalonate + ATP = (R)-5-diphosphomevalonate + ADP; Xref=Rhea:RHEA:16341 |
| GO:0019287 isopentenyl diphosphate biosynthetic process, mevalonate pathway | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT infers the IPP biosynthesis term from the same PTN001274235 node that carries phosphomevalonate kinase activity. erg8 directly catalyzes step 2/3 from mevalonate to IPP. Supporting Evidence: file:SCHPO/erg8/erg8-uniprot.txt Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 2/3. file:SCHPO/erg8/erg8-uniprot.txt Reaction=(R)-5-phosphomevalonate + ATP = (R)-5-diphosphomevalonate + ADP; Xref=Rhea:RHEA:16341 |
| GO:0019287 isopentenyl diphosphate biosynthetic process, mevalonate pathway | IEA GO_REF:0000041 | ACCEPT | Summary: UniPathway mapping to IPP biosynthesis via the mevalonate pathway. Correct core BP. Supporting Evidence: file:SCHPO/erg8/erg8-uniprot.txt Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 2/3. |
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