fps1 (SPAC6F12.13c; UniProt O14230) encodes the essential farnesyl diphosphate synthase (FPS; EC 2.5.1.10) of fission yeast. It condenses DMAPP with two IPP units, through geranyl diphosphate, to (2E,6E)-farnesyl diphosphate, the branch-point precursor of sterols, dolichol, ubiquinone, heme A and farnesylated proteins. fps1 rescues S. cerevisiae FPS deficiency and E. coli ispA. S. pombe has no separate GGPP synthase: instead, Fps1 binds the FPS-like Spo9 to form a heteromeric complex with geranylgeranyl diphosphate synthase activity, which is needed for protein geranylgeranylation and forespore membrane growth.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004311 geranylgeranyl diphosphate synthase activity | IDA PMID:17596513 Geranylgeranyl diphosphate synthase in fission yeast is a he... | ACCEPT | Summary: Fps1 and Spo9 alone have no clear GGPS activity in E. coli, but together they form a complex with GGPS activity. contributes_to is the correct qualifier, since S. pombe has no separate GGPS gene. Supporting Evidence: PMID:17596513 the two proteins together form a complex with GGPS activity PMID:17596513 coexpression of both genes induces such |
| GO:0004337 (2E,6E)-farnesyl diphosphate synthase activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference; Fps1 is the true FPS of S. pombe. Supporting Evidence: file:SCHPO/fps1/fps1-uniprot.txt of isopentenyl pyrophosphate with dimethylallyl pyrophosphate, and then PMID:17596513 indicating that S. pombe Fps1 in fact functions as an FPS |
| GO:0004337 (2E,6E)-farnesyl diphosphate synthase activity | IDA PMID:17596513 Geranylgeranyl diphosphate synthase in fission yeast is a he... | ACCEPT | Summary: fps1 rescues S. cerevisiae FPS-deficient lethality and restores ubiquinone synthesis in an E. coli ispA mutant. Supporting Evidence: PMID:17596513 Expression of fps1(+), but not spo9(+), suppresses the lethality of a PMID:17596513 indicating that S. pombe Fps1 in fact functions as an FPS |
| GO:0004337 (2E,6E)-farnesyl diphosphate synthase activity | IEA GO_REF:0000120 | ACCEPT | Summary: EC/RHEA mapping; consistent. Supporting Evidence: file:SCHPO/fps1/fps1-uniprot.txt of isopentenyl pyrophosphate with dimethylallyl pyrophosphate, and then |
| GO:0004659 prenyltransferase activity | IEA GO_REF:0000002 | MODIFY | Summary: Generic InterPro-derived parent of the specific activity, which is already annotated. Reason: Too general. Proposed replacements: (2E,6E)-farnesyl diphosphate synthase activity Supporting Evidence: file:SCHPO/fps1/fps1-uniprot.txt of isopentenyl pyrophosphate with dimethylallyl pyrophosphate, and then |
| GO:0005634 nucleus | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | KEEP AS NON CORE | Summary: ORFeome YFP screen recorded nuclear signal for fps1. Soluble metabolic enzymes often show diffuse nucleocytoplasmic signal in this screen; no nuclear role is known. Reason: Experimental HDA kept, but not part of the core function. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005634 nucleus | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: UniProt location mapping of the nuclear screen signal; no nuclear role known. Reason: Not part of the core function. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005737 cytoplasm | EXP PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: Cytoplasmic signal in the ORFeome screen. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt subcellular-location mapping (cytoplasm), derived from the localisation screen. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005783 endoplasmic reticulum | ISO GO_REF:0000024 | KEEP AS NON CORE | Summary: Transfer of ER location from S. cerevisiae ERG20. FPS is soluble; ER association in S. cerevisiae reflects proximity to sterol synthesis. No S. pombe data. Reason: Not part of the core function. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005829 cytosol | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: ORFeome YFP screen placed fps1 in the cytosol, consistent with a soluble metabolic enzyme. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005829 cytosol | IBA GO_REF:0000033 | ACCEPT | Summary: Phylogenetic inference of location; agrees with the localisation screen. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0008299 isoprenoid biosynthetic process | IEA GO_REF:0000002 | TOO GENERAL | Summary: Correct but broad parent of FPP biosynthesis, which is also annotated. Supporting Evidence: file:SCHPO/fps1/fps1-uniprot.txt of isopentenyl pyrophosphate with dimethylallyl pyrophosphate, and then |
| GO:0016765 transferase activity, transferring alkyl or aryl (other than methyl) groups | IEA GO_REF:0000002 | MODIFY | Summary: Generic InterPro-derived parent of the specific activity, which is already annotated. Reason: Too general. Proposed replacements: (2E,6E)-farnesyl diphosphate synthase activity Supporting Evidence: file:SCHPO/fps1/fps1-uniprot.txt of isopentenyl pyrophosphate with dimethylallyl pyrophosphate, and then |
| GO:0033384 geranyl diphosphate biosynthetic process | IEA GO_REF:0000041 | KEEP AS NON CORE | Summary: GPP is the enzyme-bound intermediate of the two-step FPS reaction; correct but not the end product. Supporting Evidence: file:SCHPO/fps1/fps1-uniprot.txt of isopentenyl pyrophosphate with dimethylallyl pyrophosphate, and then |
| GO:0033386 geranylgeranyl diphosphate biosynthetic process | IC PMID:17596513 Geranylgeranyl diphosphate synthase in fission yeast is a he... | ACCEPT | Summary: Fps1 is part of the Fps1-Spo9 complex that makes GGPP. Supporting Evidence: PMID:17596513 the two proteins together form a complex with GGPS activity |
| GO:0045337 trans, trans-farnesyl diphosphate biosynthetic process | EXP PMID:17596513 Geranylgeranyl diphosphate synthase in fission yeast is a he... | ACCEPT | Summary: Core process; fps1 is essential and functions as the cellular FPS. Supporting Evidence: PMID:17596513 indicating that S. pombe Fps1 in fact functions as an FPS PMID:17596513 Fps1 is essential for |
| GO:0045337 trans, trans-farnesyl diphosphate biosynthetic process | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference; consistent. Supporting Evidence: file:SCHPO/fps1/fps1-uniprot.txt of isopentenyl pyrophosphate with dimethylallyl pyrophosphate, and then |
| GO:0045337 trans, trans-farnesyl diphosphate biosynthetic process | IEA GO_REF:0000041 | ACCEPT | Summary: UniPathway mapping; consistent. Supporting Evidence: file:SCHPO/fps1/fps1-uniprot.txt of isopentenyl pyrophosphate with dimethylallyl pyrophosphate, and then |
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