gcv1 (SPAC31G5.14; UniProt O14110) encodes the mitochondrial aminomethyltransferase, the T protein of the glycine cleavage system (EC 2.1.2.10). It accepts the aminomethyl group carried on the lipoyl arm of the H protein (gcv3), transfers the one-carbon unit to tetrahydrofolate to form 5,10-methylene-THF, and releases ammonia; the reduced lipoyl arm is then reoxidised by dihydrolipoyl dehydrogenase (dld1). With the P protein (gcv2), the T protein makes the glycine cleavage system a mitochondrial source of one-carbon units for folate metabolism. Its function is assigned by orthology to S. cerevisiae GCV1 and the GcvT family; no S. pombe-specific biochemistry has been reported.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004047 aminomethyltransferase activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference of aminomethyltransferase activity from experimentally characterised GcvT orthologs (S. cerevisiae GCV1, human AMT). gcv1 is a single-copy GcvT-family member with the conserved folate-binding fold; the inference is sound. Supporting Evidence: file:SCHPO/gcv1/gcv1-uniprot.txt Reaction=N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[protein] + (6S)-5,6,7,8-tetrahydrofolate = N(6)-[(R)-dihydrolipoyl]-L-lysyl- [protein] + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NH4(+) |
| GO:0004047 aminomethyltransferase activity | IEA GO_REF:0000120 | ACCEPT | Summary: EC 2.1.2.10 / RHEA:16945 mapping from the GcvT-family InterPro signature; consistent with the IBA. Supporting Evidence: file:SCHPO/gcv1/gcv1-uniprot.txt Reaction=N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[protein] + (6S)-5,6,7,8-tetrahydrofolate = N(6)-[(R)-dihydrolipoyl]-L-lysyl- [protein] + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NH4(+) |
| GO:0005739 mitochondrion | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: ORFeome YFP localisation screen placed gcv1 in mitochondria, the expected location for the glycine cleavage system. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein. |
| GO:0005739 mitochondrion | IBA GO_REF:0000033 | ACCEPT | Summary: Phylogenetic inference of mitochondrial location, agrees with the HDA. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein. |
| GO:0005739 mitochondrion | IEA GO_REF:0000120 | ACCEPT | Summary: UniProt subcellular-location mapping; agrees with the screen and the IBA. Supporting Evidence: file:SCHPO/gcv1/gcv1-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}. |
| GO:0005759 mitochondrial matrix | IC GO_REF:0000036 | ACCEPT | Summary: Inferred by curator from the glycine cleavage process, which is a mitochondrial-matrix process in eukaryotes. Appropriate refinement of the mitochondrion annotations. Supporting Evidence: file:SCHPO/gcv1/gcv1-uniprot.txt FUNCTION: The glycine cleavage system catalyzes the degradation of glycine. |
| GO:0005960 glycine cleavage complex | IEA GO_REF:0000002 | ACCEPT | Summary: GcvT is one of the four glycine cleavage system proteins; part_of the complex is correct. Supporting Evidence: file:SCHPO/gcv1/gcv1-uniprot.txt SUBUNIT: The glycine cleavage system is composed of four proteins: P, T, L and H. |
| GO:0005960 glycine cleavage complex | ISS GO_REF:0000024 | ACCEPT | Summary: Curator transfer from an experimentally characterised ortholog; consistent with the InterPro mapping. Supporting Evidence: file:SCHPO/gcv1/gcv1-uniprot.txt SUBUNIT: The glycine cleavage system is composed of four proteins: P, T, L and H. |
| GO:0006546 glycine catabolic process | IEA GO_REF:0000002 | MODIFY | Summary: True but broad parent of the glycine cleavage process. Reason: Correct but general: glycine catabolic process is an ancestor of GO:0019464 glycine decarboxylation via glycine cleavage system, which this gene already carries and which is the process the gene product directly performs a step of. MODIFY to that descendant; applied the same way to every P-, H-, T- and L-protein review in the glycine cleavage system module. Proposed replacements: glycine decarboxylation via glycine cleavage system Supporting Evidence: file:SCHPO/gcv1/gcv1-uniprot.txt FUNCTION: The glycine cleavage system catalyzes the degradation of glycine. |
| GO:0019464 glycine decarboxylation via glycine cleavage system | IBA GO_REF:0000033 | ACCEPT | Summary: Core process: the T protein transfers the methylene unit to tetrahydrofolate and releases ammonia, completing glycine cleavage. Supporting Evidence: file:SCHPO/gcv1/gcv1-uniprot.txt FUNCTION: The glycine cleavage system catalyzes the degradation of glycine. file:SCHPO/gcv1/gcv1-uniprot.txt Reaction=N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[protein] + (6S)-5,6,7,8-tetrahydrofolate = N(6)-[(R)-dihydrolipoyl]-L-lysyl- [protein] + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NH4(+) |
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Download this section (compressed HTML)Q: Is the S. pombe glycine cleavage system needed to supply one-carbon units on serine- or glycine-limited media, as in S. cerevisiae, given that gcv1 deletion has no reported phenotype?
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