gcv2 (SPAC13G6.06c; UniProt Q09785) encodes the mitochondrial glycine dehydrogenase (decarboxylating), the pyridoxal 5'-phosphate-dependent P protein of the glycine cleavage system (EC 1.4.4.2). It decarboxylates glycine, releasing CO2, and transfers the remaining methylamine group to the lipoyl arm of the H protein (gcv3), which carries it to the T protein (gcv1). The system is a mitochondrial source of one-carbon units (5,10-methylene-THF). Localisation to mitochondria was seen in a genome-wide screen; catalytic function is assigned by orthology to S. cerevisiae GCV2 and the GcvP family.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004375 glycine dehydrogenase (decarboxylating) activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference from characterised GcvP orthologs (S. cerevisiae GCV2, human GLDC). gcv2 is the single fission yeast GcvP and keeps the PLP-binding lysine region; sound. Supporting Evidence: file:SCHPO/gcv2/gcv2-uniprot.txt The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein. file:SCHPO/gcv2/gcv2-uniprot.txt DR NCBIfam; TIGR00461; gcvP; 1. |
| GO:0004375 glycine dehydrogenase (decarboxylating) activity | IEA GO_REF:0000120 | ACCEPT | Summary: EC 1.4.4.2 / RHEA:24304 mapping from GcvP InterPro signatures; consistent with the IBA. Supporting Evidence: file:SCHPO/gcv2/gcv2-uniprot.txt DR NCBIfam; TIGR00461; gcvP; 1. |
| GO:0005739 mitochondrion | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: ORFeome YFP screen placed gcv2 in mitochondria. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein. file:SCHPO/gcv2/gcv2-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:16823372}. |
| GO:0005739 mitochondrion | IBA GO_REF:0000033 | ACCEPT | Summary: Phylogenetic inference; agrees with the HDA. Supporting Evidence: file:SCHPO/gcv2/gcv2-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:16823372}. |
| GO:0005739 mitochondrion | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt subcellular-location mapping, itself based on the ORFeome screen. Supporting Evidence: file:SCHPO/gcv2/gcv2-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:16823372}. |
| GO:0005759 mitochondrial matrix | IC GO_REF:0000036 | ACCEPT | Summary: Curator inference from the glycine cleavage process, which runs in the mitochondrial matrix. Supporting Evidence: file:SCHPO/gcv2/gcv2-uniprot.txt FUNCTION: The glycine cleavage system catalyzes the degradation of glycine. |
| GO:0005960 glycine cleavage complex | ISO GO_REF:0000024 | ACCEPT | Summary: GcvP is the P subunit of the glycine cleavage system. Supporting Evidence: file:SCHPO/gcv2/gcv2-uniprot.txt The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein. |
| GO:0006544 glycine metabolic process | IEA GO_REF:0000002 | MODIFY | Summary: True but broad parent of the glycine cleavage process. Reason: Correct but general: glycine metabolic process is an ancestor of GO:0019464 glycine decarboxylation via glycine cleavage system, which this gene already carries and which is the process the gene product directly performs a step of. MODIFY to that descendant; applied the same way to every P-, H-, T- and L-protein review in the glycine cleavage system module. Proposed replacements: glycine decarboxylation via glycine cleavage system Supporting Evidence: file:SCHPO/gcv2/gcv2-uniprot.txt The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein. |
| GO:0006546 glycine catabolic process | IEA GO_REF:0000002 | MODIFY | Summary: True but broad parent of the glycine cleavage process. Reason: Correct but general: glycine catabolic process is an ancestor of GO:0019464 glycine decarboxylation via glycine cleavage system, which this gene already carries and which is the process the gene product directly performs a step of. MODIFY to that descendant; applied the same way to every P-, H-, T- and L-protein review in the glycine cleavage system module. Proposed replacements: glycine decarboxylation via glycine cleavage system Supporting Evidence: file:SCHPO/gcv2/gcv2-uniprot.txt The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein. |
| GO:0019464 glycine decarboxylation via glycine cleavage system | IBA GO_REF:0000033 | ACCEPT | Summary: Core process: the P protein decarboxylates glycine and loads the methylamine onto the H-protein lipoyl arm. Supporting Evidence: file:SCHPO/gcv2/gcv2-uniprot.txt The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein. |
Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)