gcv3

Gene Description

gcv3 (SPBP19A11.01; UniProt Q9HDV9) encodes the mitochondrial H protein of the glycine cleavage system. It carries a covalently attached lipoyl cofactor that accepts the methylamine group from glycine decarboxylation by the P protein (gcv2), delivers it to the T protein (gcv1) for transfer to tetrahydrofolate, and is reoxidised by dihydrolipoyl dehydrogenase (dld1). In S. cerevisiae the orthologous GCV3 is also needed to lipoylate the E2 subunits of pyruvate and 2-oxoglutarate dehydrogenases; whether S. pombe gcv3 has that second role is untested. Mitochondrial localisation comes from a genome-wide screen; function is assigned by orthology.

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0005739 mitochondrion	HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ...	ACCEPT	Summary: ORFeome YFP screen placed gcv3 in mitochondria. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein. file:SCHPO/gcv3/gcv3-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269\|PubMed:16823372}.
GO:0005739 mitochondrion	IBA GO_REF:0000033	ACCEPT	Summary: Phylogenetic inference; agrees with the HDA. Supporting Evidence: file:SCHPO/gcv3/gcv3-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269\|PubMed:16823372}.
GO:0005739 mitochondrion	IEA GO_REF:0000044	ACCEPT	Summary: UniProt subcellular-location mapping, based on the ORFeome screen. Supporting Evidence: file:SCHPO/gcv3/gcv3-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269\|PubMed:16823372}.
GO:0005759 mitochondrial matrix	IC GO_REF:0000036	ACCEPT	Summary: Curator inference from the glycine cleavage process (mitochondrial matrix). Supporting Evidence: file:SCHPO/gcv3/gcv3-uniprot.txt FUNCTION: The glycine cleavage system (glycine decarboxylase complex) catalyzes the degradation of glycine.
GO:0005960 glycine cleavage complex	IBA GO_REF:0000033	ACCEPT	Summary: The lipoylated H protein is the central carrier subunit of the glycine cleavage system. Supporting Evidence: file:SCHPO/gcv3/gcv3-uniprot.txt SUBUNIT: Component of the glycine decarboxylase complex (GDC), which is composed of four proteins: P, T, L and H.
GO:0005960 glycine cleavage complex	IEA GO_REF:0000002	ACCEPT	Summary: InterPro GcvH mapping; agrees with the IBA. Supporting Evidence: file:SCHPO/gcv3/gcv3-uniprot.txt SUBUNIT: Component of the glycine decarboxylase complex (GDC), which is composed of four proteins: P, T, L and H.
GO:0019464 glycine decarboxylation via glycine cleavage system	IBA GO_REF:0000033	ACCEPT	Summary: Core process: the H protein carries the aminomethyl intermediate between the P and T proteins and is reoxidised by L protein. Supporting Evidence: file:SCHPO/gcv3/gcv3-uniprot.txt The H protein shuttles the methylamine group of glycine from the P protein to the T protein (By similarity).
GO:0019464 glycine decarboxylation via glycine cleavage system	IEA GO_REF:0000002	ACCEPT	Summary: InterPro mapping; agrees with the IBA. Supporting Evidence: file:SCHPO/gcv3/gcv3-uniprot.txt The H protein shuttles the methylamine group of glycine from the P protein to the T protein (By similarity).
GO:0140104 molecular carrier activity	NAS GO_REF:0000051	ACCEPT	Summary: The H protein is a non-catalytic carrier: its covalently bound lipoyl arm carries the methylamine intermediate between the P and T proteins and is then reoxidised by the L protein. Molecular carrier activity is the appropriate MF. In S. cerevisiae, GCV3 also supplies lipoyl groups for lipoylation of other 2-oxoacid dehydrogenase E2 subunits; that role is not tested in S. pombe. Supporting Evidence: file:SCHPO/gcv3/gcv3-uniprot.txt The H protein shuttles the methylamine group of glycine from the P protein to the T protein (By similarity). file:SCHPO/gcv3/gcv3-uniprot.txt Note=Binds 1 lipoyl cofactor covalently. {ECO:0000250};

Core Functions

Lipoyl-carrier H protein of the glycine cleavage system, shuttling the aminomethyl intermediate from the P protein to the T protein. Core MF is the accepted GO:0140104 molecular carrier activity (NAS), matching the glycine_cleavage_system module and the other H-protein reviews.

Molecular Function:

molecular carrier activity

Directly Involved In:

glycine decarboxylation via glycine cleavage system

Cellular Locations:

mitochondrial matrix

In Complex:

glycine cleavage complex

Supporting Evidence:

file:SCHPO/gcv3/gcv3-uniprot.txt
The H protein shuttles the methylamine group of glycine from the P protein to the T protein (By similarity).
file:SCHPO/gcv3/gcv3-uniprot.txt
Note=Binds 1 lipoyl cofactor covalently. {ECO:0000250};
file:SCHPO/gcv3/gcv3-uniprot.txt
SUBUNIT: Component of the glycine decarboxylase complex (GDC), which is composed of four proteins: P, T, L and H.
file:SCHPO/gcv3/gcv3-uniprot.txt
SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:16823372}.
PMID:16823372
we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein.

Gene Description

Existing Annotations Review

Core Functions

Lipoyl-carrier H protein of the glycine cleavage system, shuttling the aminomethyl intermediate from the P protein to the T protein. Core MF is the accepted GO:0140104 molecular carrier activity (NAS), matching the glycine_cleavage_system module and the other H-protein reviews.

References

Suggested Questions for Experts

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