gdh2 (SPCC132.04c; UniProt Q9USN5) encodes the NAD-specific glutamate dehydrogenase (EC 1.4.1.2), the large fungal type of GDH. It oxidatively deaminates glutamate to 2-oxoglutarate and ammonium, the catabolic counterpart of NADP-GDH (gdh1), releasing nitrogen from glutamate when cells grow on poor nitrogen sources. NAD-GDH activity has been measured in S. pombe, and the gene is assigned by orthology to S. cerevisiae GDH2.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004352 L-glutamate dehydrogenase (NAD+) activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference; consistent. Supporting Evidence: PMID:28982178 Assay of enzyme activitiesNADP-GDH and NAD-GDH activities were determined by the method of Doherty [15] with minor modifications. |
| GO:0004352 L-glutamate dehydrogenase (NAD+) activity | IDA PMID:28982178 Filamentous invasive growth of mutants of the genes encoding... | ACCEPT | Summary: NAD-GDH activity measured in wild-type and deletion strains. Supporting Evidence: PMID:28982178 Assay of enzyme activitiesNADP-GDH and NAD-GDH activities were determined by the method of Doherty [15] with minor modifications. PMID:28982178 The gln1Ξ and glt1Ξ alleles lack the entire ORFs, and the gdh1Ξ and gdh2Ξ alleles contain only the last two and eight amino acids of the ORFs, respectively (S1 Fig). |
| GO:0004352 L-glutamate dehydrogenase (NAD+) activity | IEA GO_REF:0000120 | ACCEPT | Summary: EC/ARBA mapping; consistent. Supporting Evidence: PMID:28982178 Assay of enzyme activitiesNADP-GDH and NAD-GDH activities were determined by the method of Doherty [15] with minor modifications. |
| GO:0005737 cytoplasm | IEA GO_REF:0000120 | ACCEPT | Summary: ARBA/UniProt mapping to cytoplasm; fungal NAD-GDH (S. cerevisiae Gdh2) is cytosolic. Supporting Evidence: PMID:28982178 NAD-specific glutamate dehydrogenase (NAD-GDH) has a catabolic role. |
| GO:0005739 mitochondrion | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: PAINT inference from animal/plant mitochondrial GDH. Fungal NAD-GDH is a distinct large-subunit cytosolic enzyme (S. cerevisiae Gdh2); mitochondrial location is not shown in S. pombe. Reason: Location from a different GDH lineage. Supporting Evidence: PMID:28982178 NAD-specific glutamate dehydrogenase (NAD-GDH) has a catabolic role. |
| GO:0005759 mitochondrial matrix | IC GO_REF:0000111 | KEEP AS NON CORE | Summary: Curator inference from the activity; as above. Reason: As above. Supporting Evidence: PMID:28982178 NAD-specific glutamate dehydrogenase (NAD-GDH) has a catabolic role. |
| GO:0006520 amino acid metabolic process | IEA GO_REF:0000002 | TOO GENERAL | Summary: Broad parent. Supporting Evidence: PMID:28982178 NAD-specific glutamate dehydrogenase (NAD-GDH) has a catabolic role. |
| GO:0006538 L-glutamate catabolic process | EXP PMID:28982178 Filamentous invasive growth of mutants of the genes encoding... | ACCEPT | Summary: NAD-GDH has a catabolic role, deaminating glutamate. Supporting Evidence: PMID:28982178 NAD-specific glutamate dehydrogenase (NAD-GDH) has a catabolic role. |
| GO:0006538 L-glutamate catabolic process | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference; consistent. Supporting Evidence: PMID:28982178 NAD-specific glutamate dehydrogenase (NAD-GDH) has a catabolic role. |
| GO:0006538 L-glutamate catabolic process | IEA GO_REF:0000120 | ACCEPT | Summary: ARBA mapping; consistent. Supporting Evidence: PMID:28982178 NAD-specific glutamate dehydrogenase (NAD-GDH) has a catabolic role. |
| GO:0016491 oxidoreductase activity | IEA GO_REF:0000002 | MODIFY | Summary: Generic InterPro-derived parent of the specific activity, which is already annotated. Reason: Too general. Proposed replacements: L-glutamate dehydrogenase (NAD+) activity Supporting Evidence: PMID:28982178 Assay of enzyme activitiesNADP-GDH and NAD-GDH activities were determined by the method of Doherty [15] with minor modifications. |
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