lys12 (SPAC31G5.04; UniProt O14104) encodes homoisocitrate dehydrogenase (EC 1.1.1.87), an NAD+- and Mg2+-dependent member of the isocitrate dehydrogenase family. It oxidatively decarboxylates homoisocitrate to 2-oxoadipate, the last mitochondrial step of the alpha-aminoadipate pathway. Function and mitochondrial location are assigned by orthology to S. cerevisiae LYS12; deletion causes lysine auxotrophy. The ORFeome screen recorded cytoplasmic signal for a tagged construct.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000287 magnesium ion binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: Mg2+ cofactor of the isocitrate dehydrogenase-family reaction; subsidiary. Supporting Evidence: file:SCHPO/lys12/lys12-uniprot.txt EC=1.1.1.87 |
| GO:0005737 cytoplasm | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | KEEP AS NON CORE | Summary: ORFeome screen recorded cytoplasmic signal. Homoisocitrate dehydrogenase is mitochondrial in S. cerevisiae (Lys12); the tag may affect targeting. Reason: Conflicts with expected mitochondrial location. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: UniProt mapping of the screen signal; as above. Reason: As above. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005739 mitochondrion | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference of mitochondrial location, as for S. cerevisiae Lys12. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005759 mitochondrial matrix | IC GO_REF:0000111 | ACCEPT | Summary: Curator inference from the activity; consistent with the mitochondrial pathway segment. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0009085 L-lysine biosynthetic process | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference; consistent. Supporting Evidence: PMID:17622533 Mutants that were auxotrophic for arginine, asparagine, cysteine, lysine, methionine and phenylalanine were isolated. |
| GO:0009085 L-lysine biosynthetic process | IMP PMID:17622533 Six new amino acid-auxotrophic markers for targeted gene int... | ACCEPT | Summary: Lysine auxotroph from the marker screen. Supporting Evidence: PMID:17622533 Mutants that were auxotrophic for arginine, asparagine, cysteine, lysine, methionine and phenylalanine were isolated. |
| GO:0009085 L-lysine biosynthetic process | IMP PMID:20537132 Global fitness profiling of fission yeast deletion strains b... | ACCEPT | Summary: Deletion-strain fitness profiling (lysine requirement). Supporting Evidence: PMID:20537132 Here we use deep sequencing to accurately characterize the barcode seque |
| GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor | IEA GO_REF:0000002 | MODIFY | Summary: Generic InterPro-derived parent of the specific activity, which is already annotated. Reason: Too general. Proposed replacements: homoisocitrate dehydrogenase activity Supporting Evidence: file:SCHPO/lys12/lys12-uniprot.txt EC=1.1.1.87 |
| GO:0047046 homoisocitrate dehydrogenase activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference from characterised Lys12-type enzymes; sound. Supporting Evidence: file:SCHPO/lys12/lys12-uniprot.txt EC=1.1.1.87 |
| GO:0047046 homoisocitrate dehydrogenase activity | IEA GO_REF:0000120 | ACCEPT | Summary: EC/RHEA mapping; consistent. Supporting Evidence: file:SCHPO/lys12/lys12-uniprot.txt EC=1.1.1.87 |
| GO:0051287 NAD binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: NAD+ cosubstrate; subsidiary. Supporting Evidence: file:SCHPO/lys12/lys12-uniprot.txt EC=1.1.1.87 |
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