nad1 (SPCC1235.04c; UniProt O74841) encodes FAD synthetase (FMN adenylyltransferase; EC 2.7.7.2), which adenylates FMN with ATP to form FAD, the second step in making flavin cofactors after riboflavin kinase (fmn1). Function is assigned by orthology to S. cerevisiae FAD1; it is cytosolic in a genome-wide screen.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003824 catalytic activity | IEA GO_REF:0000002 | MODIFY | Summary: Generic InterPro-derived parent of the specific FMN adenylyltransferase activity, which is already annotated. Reason: The term is correct but uninformative. UniProt assigns the FMN adenylation reaction to nad1 (EC 2.7.7.2) by similarity to S. cerevisiae Fad1 (ECO:0000250|UniProtKB:P38913), and the protein belongs to the FAD1 subfamily of the PAPS reductase family (PANTHER PTHR23293:SF9). The replacement GO:0003919 is already on the gene (ACCEPT). Proposed replacements: FMN adenylyltransferase activity Supporting Evidence: file:SCHPO/nad1/nad1-uniprot.txt Reaction=FMN + ATP + H(+) = FAD + diphosphate file:SCHPO/nad1/nad1-uniprot.txt PANTHER; PTHR23293:SF9; FAD SYNTHASE; 1. |
| GO:0003919 FMN adenylyltransferase activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference from characterised FAD synthetases (S. cerevisiae FAD1, human FLAD1); sound. Reason: No S. pombe assay is cached. UniProt curates the FMN adenylylation function and reaction by similarity to S. cerevisiae FAD1 (ECO:0000250|UniProtKB:P38913), and the protein is placed in the FAD1 subfamily, consistent with the PAINT node seeded by FAD1 and human FLAD1. Supporting Evidence: file:SCHPO/nad1/nad1-uniprot.txt Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme. file:SCHPO/nad1/nad1-uniprot.txt Belongs to the PAPS reductase family. FAD1 subfamily. |
| GO:0003919 FMN adenylyltransferase activity | IEA GO_REF:0000120 | ACCEPT | Summary: EC/RHEA mapping; consistent. Reason: The mapped reaction (RHEA:17237, EC 2.7.7.2) is curated in UniProt by similarity to S. cerevisiae FAD1 (ECO:0000250|UniProtKB:P38913); the protein is in the FAD1 subfamily. Supporting Evidence: file:SCHPO/nad1/nad1-uniprot.txt Reaction=FMN + ATP + H(+) = FAD + diphosphate file:SCHPO/nad1/nad1-uniprot.txt Belongs to the PAPS reductase family. FAD1 subfamily. |
| GO:0003919 FMN adenylyltransferase activity | ISS GO_REF:0000024 | ACCEPT | Summary: Curator transfer from S. cerevisiae FAD1 (P38913). Reason: The ISS transfer is from S. cerevisiae FAD1 (P38913); UniProt's curated function statement cites the same ortholog (ECO:0000250|UniProtKB:P38913). The evidence is FAD1's, extended to nad1 by FAD1-subfamily membership. Supporting Evidence: file:SCHPO/nad1/nad1-uniprot.txt Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme. file:SCHPO/nad1/nad1-uniprot.txt Belongs to the PAPS reductase family. FAD1 subfamily. |
| GO:0005634 nucleus | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | KEEP AS NON CORE | Summary: ORFeome YFP screen recorded nuclear signal for nad1. Soluble metabolic enzymes often show diffuse nucleocytoplasmic signal in this screen; no nuclear role is known. Reason: Experimental HDA kept, but not part of the core function. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005634 nucleus | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: UniProt location mapping of the nuclear screen signal; no nuclear role known. Reason: Not part of the core function. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005739 mitochondrion | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: UniProt location mapping by similarity to S. cerevisiae Fad1; S. pombe nad1 is cytosolic in the screen. Reason: Not established for S. pombe. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005739 mitochondrion | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Curator transfer of mitochondrial location from S. cerevisiae FAD1; as above. Reason: As above. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005829 cytosol | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: ORFeome YFP screen placed nad1 in the cytosol, consistent with a soluble metabolic enzyme. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005829 cytosol | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt subcellular-location mapping (cytosol), derived from the localisation screen. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0006747 FAD biosynthetic process | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference; FMN -> FAD. Reason: UniProt curates the pathway step (FAD from FMN, step 1/1) by similarity to S. cerevisiae FAD1 (ECO:0000250|UniProtKB:P38913); FAD1-subfamily membership supports the transfer. Supporting Evidence: file:SCHPO/nad1/nad1-uniprot.txt PATHWAY: Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1. file:SCHPO/nad1/nad1-uniprot.txt Belongs to the PAPS reductase family. FAD1 subfamily. |
| GO:0006747 FAD biosynthetic process | IEA GO_REF:0000041 | ACCEPT | Summary: UniPathway mapping; consistent. Reason: The UniPathway mapping matches the UniProt pathway statement, curated by similarity to S. cerevisiae FAD1 (ECO:0000250|UniProtKB:P38913). Supporting Evidence: file:SCHPO/nad1/nad1-uniprot.txt PATHWAY: Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1. |
| GO:0046444 FMN metabolic process | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: FMN is the substrate that FAD synthase consumes, so the term is true; FAD biosynthesis is the specific, core process. Reason: FAD synthase adenylates FMN to FAD (by similarity to S. cerevisiae Fad1, ECO:0000250|UniProtKB:P38913, the ISS source), which is a reaction of FMN metabolism. FAD biosynthetic process is not a descendant of FMN metabolic process, so this row is not entailed by the core term. It is kept as a true but peripheral description of the same reaction. Supporting Evidence: file:SCHPO/nad1/nad1-uniprot.txt Catalyzes the adenylation of flavin mononucleotide (FMN) to file:SCHPO/nad1/nad1-uniprot.txt PATHWAY: Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step |
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