nip7

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0003723 RNA binding	IEA GO_REF:0000002	ACCEPT	Summary: Nip7 is an RNA-binding pre-60S assembly factor. Reason: The conserved PUA RNA-binding architecture and direct presence of Q1MTQ9 in an early pre-60S particle support RNA binding in assembly. This is not an inference of kinase regulation from the broad mixed PANTHER family. Supporting Evidence: file:SCHPO/nip7/nip7-8ESQ-entities.json "pdbx_description": "60S ribosome subunit biogenesis protein nip7" file:SCHPO/nip7/nip7-uniprot.txt DR InterPro; IPR002478; PUA.
GO:0003723 RNA binding	NAS GO_REF:0000051	ACCEPT	Summary: Nip7 is an RNA-binding pre-60S assembly factor. Reason: The conserved PUA RNA-binding architecture and direct presence of Q1MTQ9 in an early pre-60S particle support RNA binding in assembly. This is not an inference of kinase regulation from the broad mixed PANTHER family. Supporting Evidence: file:SCHPO/nip7/nip7-8ESQ-entities.json "pdbx_description": "60S ribosome subunit biogenesis protein nip7" file:SCHPO/nip7/nip7-uniprot.txt DR InterPro; IPR002478; PUA.
GO:0005634 nucleus	HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ...	MODIFY	Summary: The core nuclear localization is the nucleolus. Reason: The target nucleolar HDA annotation and early pre-60S complex establish a more specific site of action than nucleus. Proposed replacements: nucleolus Supporting Evidence: file:SCHPO/nip7/nip7-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269\|PubMed:16823372}. Nucleus, CC nucleolus {ECO:0000269\|PubMed:16823372}.
GO:0005634 nucleus	IEA GO_REF:0000002	MODIFY	Summary: The core nuclear localization is the nucleolus. Reason: The target nucleolar HDA annotation and early pre-60S complex establish a more specific site of action than nucleus. Proposed replacements: nucleolus Supporting Evidence: file:SCHPO/nip7/nip7-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269\|PubMed:16823372}. Nucleus, CC nucleolus {ECO:0000269\|PubMed:16823372}.
GO:0005730 nucleolus	HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ...	ACCEPT	Summary: Nip7 functions in nucleolar pre-60S assembly. Reason: The target localization annotation and the primary pre-60S structural study are concordant. The cached localization atlas lacks full text, so the target imaging assignment is accepted as curated rather than independently re-read. Supporting Evidence: file:SCHPO/nip7/nip7-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269\|PubMed:16823372}. Nucleus, CC nucleolus {ECO:0000269\|PubMed:16823372}.
GO:0005730 nucleolus	IBA GO_REF:0000033	ACCEPT	Summary: Nip7 functions in nucleolar pre-60S assembly. Reason: The target localization annotation and the primary pre-60S structural study are concordant. The cached localization atlas lacks full text, so the target imaging assignment is accepted as curated rather than independently re-read. Supporting Evidence: file:SCHPO/nip7/nip7-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269\|PubMed:16823372}. Nucleus, CC nucleolus {ECO:0000269\|PubMed:16823372}.
GO:0005730 nucleolus	IEA GO_REF:0000044	ACCEPT	Summary: Nip7 functions in nucleolar pre-60S assembly. Reason: The target localization annotation and the primary pre-60S structural study are concordant. The cached localization atlas lacks full text, so the target imaging assignment is accepted as curated rather than independently re-read. Supporting Evidence: file:SCHPO/nip7/nip7-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269\|PubMed:16823372}. Nucleus, CC nucleolus {ECO:0000269\|PubMed:16823372}.
GO:0005737 cytoplasm	IEA GO_REF:0000044	KEEP AS NON CORE	Summary: A cytoplasmic/cytosolic pool is compatible with the target localization survey. Reason: Retain the observation as non-core; the directly characterized role is nucleolar large-subunit assembly, and cytosolic detection alone does not establish a separate translation function. Supporting Evidence: file:SCHPO/nip7/nip7-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269\|PubMed:16823372}. Nucleus, CC nucleolus {ECO:0000269\|PubMed:16823372}.
GO:0005829 cytosol	HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ...	KEEP AS NON CORE	Summary: A cytoplasmic/cytosolic pool is compatible with the target localization survey. Reason: Retain the observation as non-core; the directly characterized role is nucleolar large-subunit assembly, and cytosolic detection alone does not establish a separate translation function. Supporting Evidence: file:SCHPO/nip7/nip7-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269\|PubMed:16823372}. Nucleus, CC nucleolus {ECO:0000269\|PubMed:16823372}.
GO:0030684 preribosome	IDA PMID:36423630 Chromatin localization of nucleophosmin organizes ribosome b...	MODIFY	Summary: Nip7 occurs in an early large-subunit preribosome. Reason: RCSB entry 8ESQ links author chain l to exact accession Q1MTQ9. Although the paper foregrounds nucleophosmin, its deposited particle includes Nip7; the more specific pre-60S complex is appropriate. Proposed replacements: preribosome, large subunit precursor Supporting Evidence: file:SCHPO/nip7/nip7-8ESQ-entities.json "pdbx_description": "60S ribosome subunit biogenesis protein nip7"
GO:0030687 preribosome, large subunit precursor	IBA GO_REF:0000033	ACCEPT	Summary: Nip7 is part of the large-subunit preribosome. Reason: The exact target occurs in the deposited early pre-60S particle, independently supporting the ancestral PAINT inference. Supporting Evidence: file:SCHPO/nip7/nip7-8ESQ-entities.json "pdbx_description": "60S ribosome subunit biogenesis protein nip7"
GO:0042255 ribosome assembly	IEA GO_REF:0000002	MODIFY	Summary: Nip7 supports assembly of the large-subunit preribosome. Reason: The direct structural context resolves broad ribosome assembly/biogenesis to the early large-subunit precursor step. Proposed replacements: assembly of large subunit precursor of preribosome Supporting Evidence: file:SCHPO/nip7/nip7-8ESQ-entities.json "pdbx_description": "60S ribosome subunit biogenesis protein nip7"
GO:0042273 ribosomal large subunit biogenesis	IBA GO_REF:0000033	MODIFY	Summary: Nip7 supports assembly of the large-subunit preribosome. Reason: The direct structural context resolves broad ribosome assembly/biogenesis to the early large-subunit precursor step. Proposed replacements: assembly of large subunit precursor of preribosome Supporting Evidence: file:SCHPO/nip7/nip7-8ESQ-entities.json "pdbx_description": "60S ribosome subunit biogenesis protein nip7"
GO:1902626 assembly of large subunit precursor of preribosome	EXP PMID:36423630 Chromatin localization of nucleophosmin organizes ribosome b...	ACCEPT	Summary: Nip7 contributes to assembly of the large-subunit precursor. Reason: The primary study isolates and structures fission-yeast pre-60S particles; exact Q1MTQ9 is a component in 8ESQ. This supports the role independently of ARBA. Supporting Evidence: file:SCHPO/nip7/nip7-8ESQ-entities.json "pdbx_description": "60S ribosome subunit biogenesis protein nip7"
GO:1902626 assembly of large subunit precursor of preribosome	IEA GO_REF:0000117	ACCEPT	Summary: Nip7 contributes to assembly of the large-subunit precursor. Reason: The primary study isolates and structures fission-yeast pre-60S particles; exact Q1MTQ9 is a component in 8ESQ. This supports the role independently of ARBA. Supporting Evidence: file:SCHPO/nip7/nip7-8ESQ-entities.json "pdbx_description": "60S ribosome subunit biogenesis protein nip7"