Pgk1 (SPBC14F5.04c) is the phosphoglycerate kinase of the fission yeast Schizosaccharomyces pombe, a 414-residue monomeric (by similarity) enzyme encoded by a single gene. It catalyses the reversible, Mg2+-dependent transfer of the 1-phosphoryl group of 1,3-bisphosphoglycerate to ADP, producing 3-phosphoglycerate and ATP: the first ATP-generating step of glycolysis and, in reverse, a gluconeogenic step. The protein is cytosolic, with some nuclear signal in genome-wide localisation; a mitochondria-associated pool and a role in restraining mitochondrial acetyl-CoA synthesis are documented for the S. cerevisiae orthologue but have not been examined in S. pombe.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004618 phosphoglycerate kinase activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT IBA phosphoglycerate kinase activity; pgk1 is the single PGK gene of S. pombe and conserves the PGK fold. Supporting Evidence: file:SCHPO/pgk1/pgk1-uniprot.txt Reaction=(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl file:SCHPO/pgk1/pgk1-uniprot.txt Catalyzes one of the two ATP producing reactions in the |
| GO:0004618 phosphoglycerate kinase activity | IEA GO_REF:0000120 | ACCEPT | Summary: EC 2.7.2.3 / RHEA:14801 automated mapping; correct. Supporting Evidence: file:SCHPO/pgk1/pgk1-uniprot.txt Reaction=(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl |
| GO:0004618 phosphoglycerate kinase activity | ISS GO_REF:0000024 | ACCEPT | Summary: UniProt ISS to S. cerevisiae Pgk1 (P00560); correct by orthology for the sole S. pombe PGK. No direct S. pombe enzymology is cached. Supporting Evidence: file:SCHPO/pgk1/pgk1-uniprot.txt Reaction=(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl |
| GO:0005524 ATP binding | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: IBA ATP binding; ATP/ADP are the phosphoryl donor/acceptor substrates. Correct but part of the catalytic activity; non-core, as in the S. cerevisiae PGK1 review. Supporting Evidence: file:SCHPO/pgk1/pgk1-uniprot.txt Reaction=(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl |
| GO:0005634 nucleus | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | KEEP AS NON CORE | Summary: Genome-wide YFP localisation reported nuclear as well as cytosolic signal for Pgk1; no known nuclear function in S. pombe. Reason: High-throughput localisation; non-core. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt subcellular location (by similarity to P00560) mapping to cytoplasm; correct. Supporting Evidence: file:SCHPO/pgk1/pgk1-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P00560}. |
| GO:0005737 cytoplasm | ISS GO_REF:0000024 | ACCEPT | Summary: UniProt ISS cytoplasm from S. cerevisiae Pgk1; correct and consistent with S. pombe HDA cytosol. Supporting Evidence: file:SCHPO/pgk1/pgk1-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P00560}. |
| GO:0005739 mitochondrion | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: UniProt subcellular-location mapping to mitochondrion, itself transferred by similarity from S. cerevisiae Pgk1 mitochondrial proteomics. A minor mitochondria-associated pool is documented only in budding yeast; no S. pombe evidence. Reason: Peripheral association transferred from budding yeast; not the site of core activity. Supporting Evidence: file:SCHPO/pgk1/pgk1-uniprot.txt Mitochondrion {ECO:0000250|UniProtKB:P00560}. |
| GO:0005739 mitochondrion | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: UniProt ISS mitochondrion from S. cerevisiae Pgk1 (mitochondrial proteome detection). Kept as non-core, consistent with the S. cerevisiae PGK1 review, but untested in S. pombe where the ORFeome screen reports cytosol and nucleus. Reason: Peripheral association transferred from budding yeast; non-core. Supporting Evidence: file:SCHPO/pgk1/pgk1-uniprot.txt Mitochondrion {ECO:0000250|UniProtKB:P00560}. |
| GO:0005829 cytosol | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: ORFeome YFP localisation places Pgk1 in the cytosol. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005829 cytosol | IBA GO_REF:0000033 | ACCEPT | Summary: IBA cytosol; correct and supported by S. pombe HDA. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0006094 gluconeogenesis | IBA GO_REF:0000033 | ACCEPT | Summary: IBA gluconeogenesis; the PGK reaction is reversible and pgk1 is the only PGK, so it must operate in the gluconeogenic direction on non-fermentable carbon. Consistent with the S. cerevisiae PGK1 review. Supporting Evidence: file:SCHPO/pgk1/pgk1-uniprot.txt Catalyzes one of the two ATP producing reactions in the |
| GO:0006096 glycolysis | IBA GO_REF:0000033 | ACCEPT | Summary: IBA glycolysis; Pgk1 catalyses the first ATP-generating step of glycolysis. Supporting Evidence: file:SCHPO/pgk1/pgk1-uniprot.txt Catalyzes one of the two ATP producing reactions in the file:SCHPO/pgk1/pgk1-uniprot.txt PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D- |
| GO:0006096 glycolysis | IEA GO_REF:0000120 | ACCEPT | Summary: UniPathway/combined automated mapping to glycolysis; correct. Supporting Evidence: file:SCHPO/pgk1/pgk1-uniprot.txt PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D- |
| GO:0043531 ADP binding | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: IBA ADP binding; ADP is the phosphoryl acceptor in the glycolytic direction. Correct, non-core. Supporting Evidence: file:SCHPO/pgk1/pgk1-uniprot.txt Reaction=(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl |
| GO:0061621 obsolete canonical glycolysis | ISO GO_REF:0000024 | MODIFY | Summary: PomBase ISO from S. cerevisiae PGK1 to 'canonical glycolysis', obsoleted 2026-09-01 and replaced_by glycolysis (GO:0006096). Biology correct; term should be updated. Reason: Obsolete term; GO replaced_by GO:0006096. Propagation Review Root cause: SOURCE STALE OR MISSING Proposed replacements: glycolysis Supporting Evidence: file:SCHPO/pgk1/pgk1-uniprot.txt Catalyzes one of the two ATP producing reactions in the |
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