pnc1 (SPBC365.20c; UniProt Q9USS0) encodes nicotinamidase (EC 3.5.1.19), which deamidates nicotinamide, released by NAD+-consuming enzymes such as sirtuins, to nicotinate. This is the first step of NAD+ salvage through the Preiss-Handler route (nicotinate phosphoribosyltransferase, NaMN/NMN adenylyltransferase, NAD+ synthetase). Function is assigned by orthology to S. cerevisiae PNC1; a genome-wide screen places it in the cytosol and nucleus.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005634 nucleus | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | KEEP AS NON CORE | Summary: ORFeome YFP screen recorded nuclear signal for pnc1. Soluble metabolic enzymes often show diffuse nucleocytoplasmic signal in this screen; no nuclear role is known. Reason: Experimental HDA kept, but not part of the core function. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005634 nucleus | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: PAINT inference of nuclear location (S. cerevisiae Pnc1 is nucleocytoplasmic, where it relieves nicotinamide inhibition of sirtuins). Reason: Not established for S. pombe. Supporting Evidence: file:SCHPO/pnc1/pnc1-uniprot.txt FUNCTION: Catalyzes the deamidation of nicotinamide, an early step |
| GO:0005634 nucleus | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: UniProt location mapping of the nuclear screen signal; no nuclear role known. Reason: Not part of the core function. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt subcellular-location mapping (cytoplasm), derived from the localisation screen. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005777 peroxisome | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: UniProt location mapping (peroxisome) by similarity to S. cerevisiae Pnc1, which is co-imported into peroxisomes with Gpd1 under stress. Not shown in S. pombe. Reason: Not established for S. pombe. Supporting Evidence: file:SCHPO/pnc1/pnc1-uniprot.txt FUNCTION: Catalyzes the deamidation of nicotinamide, an early step |
| GO:0005777 peroxisome | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Curator transfer from S. cerevisiae Pnc1 (P53184); as above. Reason: As above. Supporting Evidence: file:SCHPO/pnc1/pnc1-uniprot.txt FUNCTION: Catalyzes the deamidation of nicotinamide, an early step |
| GO:0005829 cytosol | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: ORFeome YFP screen placed pnc1 in the cytosol, consistent with a soluble metabolic enzyme. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0006769 nicotinamide metabolic process | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference; nicotinamide deamidation. Supporting Evidence: file:SCHPO/pnc1/pnc1-uniprot.txt FUNCTION: Catalyzes the deamidation of nicotinamide, an early step |
| GO:0008936 nicotinamidase activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference from characterised nicotinamidases (S. cerevisiae PNC1); sound. Supporting Evidence: file:SCHPO/pnc1/pnc1-uniprot.txt Reaction=nicotinamide + H2O = nicotinate + NH4(+); |
| GO:0008936 nicotinamidase activity | IEA GO_REF:0000120 | ACCEPT | Summary: EC/RHEA mapping; consistent. Supporting Evidence: file:SCHPO/pnc1/pnc1-uniprot.txt Reaction=nicotinamide + H2O = nicotinate + NH4(+); |
| GO:0019358 nicotinate nucleotide salvage | ISO GO_REF:0000024 | ACCEPT | Summary: Transfer from S. cerevisiae PNC1: nicotinate produced feeds Preiss-Handler salvage. Supporting Evidence: file:SCHPO/pnc1/pnc1-uniprot.txt FUNCTION: Catalyzes the deamidation of nicotinamide, an early step |
| GO:0034355 NAD+ biosynthetic process via the salvage pathway | IC GO_REF:0000111 | ACCEPT | Summary: Curator inference; first step of NAD+ salvage from nicotinamide. Supporting Evidence: file:SCHPO/pnc1/pnc1-uniprot.txt FUNCTION: Catalyzes the deamidation of nicotinamide, an early step |
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