| Evidence type | Key finding | Experimental approach/system | Species | Source (authors, year, journal) | URL/DOI |
|---|---|---|---|---|---|
| Identity / annotation | SPBC14C8.14c was recovered in a GFP-fusion localization screen and annotated there as “DNA polymerase V,” matching the historical Pol5 naming used for the fission-yeast gene; however, the screen excerpt does not unambiguously assign a localization category to this entry. (pqac-00000014) | GFP-fusion genomic DNA library screen; clone TC48; fusion position 795/959 | *S. pombe* | Ding et al., 2000, *Genes to Cells* | https://doi.org/10.1046/j.1365-2443.2000.00317.x |
| Essentiality | *pol5+* is reported as essential for viability in fission yeast. (pqac-00000001, pqac-00000002) | Gene disruption and conditional-expression analyses summarized in thesis work | *S. pombe* | Nadeem, 2005, thesis | URL not available in retrieved context |
| Localization | GFP-tagged SpPol5p localized to the nucleus; nucleolar localization was proposed but not conclusively demonstrated in the thesis. (pqac-00000001, pqac-00000002) | GFP tagging / fluorescence localization | *S. pombe* | Nadeem, 2005, thesis | URL not available in retrieved context |
| Interaction / complex | SpPol5p physically interacts with the C-terminus of the cell-cycle transcription factor Cdc10p, suggesting a link between cell-cycle control and growth/rRNA production. (pqac-00000000, pqac-00000002) | Yeast two-hybrid, co-immunoprecipitation, GST pull-down | *S. pombe* | Nadeem, 2005, thesis | URL not available in retrieved context |
| Functional evidence | *pol5+* mRNA is constitutive and low abundance; overexpression perturbs rRNA production, supporting a role in rRNA synthesis/ribosome biogenesis rather than a conventional DNA polymerase role. (pqac-00000001, pqac-00000002) | Northern blot; overexpression and shut-off experiments; pulse-chase rRNA labeling | *S. pombe* | Nadeem, 2005, thesis | URL not available in retrieved context |
| PTM / essential residue | Pol5 is an Eso1 interaction partner and acetylation substrate; mutation of conserved Lys47 to Arg was lethal, indicating an essential role for this residue/modification state in viability. (pqac-00000003) | TAP purification, immunoprecipitation, LC-MS/MS, site-directed mutagenesis, tetrad analysis | *S. pombe* | Chen et al., 2017, *International Journal of Molecular Medicine* | https://doi.org/10.3892/ijmm.2017.3192 |
| Pathway context | Recent review of fission-yeast ribosomal gene regulation emphasizes TOR-responsive repression of ribosome-related transcription, starvation-induced chromatin remodeling, and rDNA heterochromatin/RNAi pathways; Pol5 is not specifically placed in these pathways in the review. (pqac-00000008, pqac-00000009, pqac-00000011) | Comparative review of primary literature | *S. pombe* | Hirai & Ohta, 2023, *Biomolecules* | https://doi.org/10.3390/biom13020288 |
| Cross-species function inference | Budding-yeast Pol5 is an essential nucleolar trans-acting factor for 60S subunit maturation; depletion causes 60S deficiency, half-mer polysomes, defective 27SB→25S processing, and impaired pre-60S export. This strongly supports annotating *S. pombe* Pol5 as a ribosome-biogenesis factor. (pqac-00000005, pqac-00000006) | Depletion and temperature-sensitive mutants; polysome analysis; pre-rRNA processing assays; genetic suppression | *S. cerevisiae* | Ramos-Sáenz et al., 2019, *RNA* | https://doi.org/10.1261/rna.072116.119 |
| Cross-species mechanistic inference | Budding-yeast Pol5 binds the 5′ ETS and domain III of 25S rRNA, promotes peptide-exit-tunnel assembly in the LSU, and supports recycling of pre-40S factors; these data argue that Pol5 family proteins act in ribosome assembly, not replicative DNA synthesis. (pqac-00000004) | Pol5 depletion, RNA binding-site mapping, pre-rRNA processing analyses | *S. cerevisiae* | Braun et al., 2020, *Nucleic Acids Research* | https://doi.org/10.1093/nar/gkz1079 |


*Table: This table compiles the strongest directly supported evidence for functional annotation of *Schizosaccharomyces pombe* Pol5 (O60094/SPBC14C8.14c), separating organism-specific findings from cross-species inference. It is useful for distinguishing firm experimental evidence in fission yeast from broader pathway context and conserved Pol5-family function.*