id: Q10222
gene_symbol: pta1
product_type: PROTEIN
status: COMPLETE
taxon:
  id: NCBITaxon:284812
  label: Schizosaccharomyces pombe (strain 972 / ATCC 24843)
description: Pta1 is a conserved noncatalytic component of the nuclear cleavage and polyadenylation factor
  complex. It supports cotranscriptional mRNA 3′-end formation and the coupling of RNA cleavage and polyadenylation
  to RNA polymerase II transcription termination. Its role is organization of the processing machinery,
  whose cleavage endonuclease and poly(A) polymerase are separate subunits.
existing_annotations:
- term:
    id: GO:0003674
    label: molecular_function
  evidence_type: ND
  original_reference_id: GO_REF:0000015
  qualifier: enables
  review:
    summary: molecular_function is supported.
    action: ACCEPT
    reason: No independently demonstrated catalytic molecular activity is assigned to this CPF scaffold.
      The ND root term records an unresolved molecular function and is not evidence against its established
      biological-process role.
    supported_by:
    - &id001
      reference_id: PMID:33711009
      supporting_text: 'Holo-CPF consists of two component complexes (Fig 1): a 10-subunit CPF core composed
        of proteins Ysh1 (the cleavage endonuclease), Pla1 (the poly(A) polymerase), Pta1, Yth1 (a zinc
        finger protein), Pfs2 (a WD repeat protein), Iss1, Cft1 (a WD repeat protein), Cft2 (a metallo-β-lactamase/β-CASP
        protein), Ctf1, and Ssu72 (a phosphoprotein phosphatase); and a 3-subunit DPS complex comprising
        Dis2 (a phosphoprotein phosphatase), Ppn1, and Swd22 (a WD repeat protein).'
- term: &id006
    id: GO:0005634
    label: nucleus
  evidence_type: HDA
  original_reference_id: PMID:16823372
  qualifier: is_active_in
  review:
    summary: nucleus is supported.
    action: ACCEPT
    reason: Pta1 is a constituent of the nuclear cotranscriptional cleavage and polyadenylation machinery,
      consistent with the curated localization screen.
    supported_by:
    - reference_id: PMID:16823372
      supporting_text: Next, we determined the localization of 4,431 proteins, corresponding to approximately
        90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein.
    - *id001
- term:
    id: GO:0005634
    label: nucleus
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  qualifier: located_in
  supporting_entities:
  - ARBA:ARBA00026330
  - UniProtKB-SubCell:SL-0191
  review:
    summary: nucleus is supported.
    action: ACCEPT
    reason: Pta1 is a constituent of the nuclear cotranscriptional cleavage and polyadenylation machinery,
      consistent with the curated localization screen.
    supported_by:
    - *id001
- term:
    id: GO:0005634
    label: nucleus
  evidence_type: NAS
  original_reference_id: PMID:33711009
  qualifier: located_in
  review:
    summary: nucleus is supported.
    action: ACCEPT
    reason: Pta1 is a constituent of the nuclear cotranscriptional cleavage and polyadenylation machinery,
      consistent with the curated localization screen.
    supported_by:
    - *id001
- term: &id005
    id: GO:0005847
    label: mRNA cleavage and polyadenylation specificity factor complex
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: part_of
  supporting_entities:
  - FB:FBgn0037371
  - PANTHER:PTN000391745
  - PomBase:SPAC1071.01c
  - SGD:S000000041
  - UniProtKB:Q57V70
  - UniProtKB:Q92797
  review:
    summary: mRNA cleavage and polyadenylation specificity factor complex is supported.
    action: ACCEPT
    reason: Fission yeast CPF contains Pta1 in its ten-subunit core. The original proteomic study underlies
      PomBase complex membership; the accessible CPF study explicitly lists Pta1 and distinguishes it
      from the catalytic Ysh1 and Pla1 subunits.
    supported_by:
    - *id001
- term:
    id: GO:0005847
    label: mRNA cleavage and polyadenylation specificity factor complex
  evidence_type: IDA
  original_reference_id: PMID:14617822
  qualifier: part_of
  review:
    summary: mRNA cleavage and polyadenylation specificity factor complex is supported.
    action: ACCEPT
    reason: Fission yeast CPF contains Pta1 in its ten-subunit core. The original proteomic study underlies
      PomBase complex membership; the accessible CPF study explicitly lists Pta1 and distinguishes it
      from the catalytic Ysh1 and Pla1 subunits. The original PMID:14617822 annotation is retained at
      its stated evidential scope; accessible source text and the independent evidence cited here are
      considered together.
    supported_by:
    - *id001
- term:
    id: GO:0005847
    label: mRNA cleavage and polyadenylation specificity factor complex
  evidence_type: NAS
  original_reference_id: PMID:33711009
  qualifier: part_of
  review:
    summary: mRNA cleavage and polyadenylation specificity factor complex is supported.
    action: ACCEPT
    reason: Fission yeast CPF contains Pta1 in its ten-subunit core. The original proteomic study underlies
      PomBase complex membership; the accessible CPF study explicitly lists Pta1 and distinguishes it
      from the catalytic Ysh1 and Pla1 subunits.
    supported_by:
    - *id001
- term:
    id: GO:0008033
    label: tRNA processing
  evidence_type: ISO
  original_reference_id: GO_REF:0000024
  qualifier: involved_in
  supporting_entities:
  - SGD:S000000041
  review:
    summary: tRNA processing is retained as an ancillary annotation.
    action: KEEP_AS_NON_CORE
    reason: Budding yeast pta1 mutants accumulate intron-containing pre-tRNAs, supporting the curated
      orthology-based tRNA-processing association. Extracts retain normal tRNA-splicing endonuclease activity,
      so this phenotype does not establish Pta1 as a tRNA nuclease or make tRNA processing its principal
      conserved biochemical role.
    supported_by:
    - reference_id: PMID:1508188
      supporting_text: PTA1 was initially defined by a UV-induced mutation, pta1-1, that causes the accumulation
        of all 10 end-trimmed, intron-containing pre-tRNAs and temperature-sensitive but osmotic-remedial
        growth.
    - &id002
      reference_id: PMID:10523662
      supporting_text: Extracts prepared from pta1 mutant strains are impaired in the cleavage and the
        poly(A) addition of both GAL7 and CYC1 substrates and exhibit little processing activity even
        after prolonged incubation. However, activity is efficiently rescued by the addition of purified
        CF II to the defective extracts.
- term: &id004
    id: GO:0030846
    label: termination of RNA polymerase II transcription, poly(A)-coupled
  evidence_type: NAS
  original_reference_id: PMID:33711009
  qualifier: involved_in
  review:
    summary: termination of RNA polymerase II transcription, poly(A)-coupled is supported.
    action: ACCEPT
    reason: CPF executes cotranscriptional mRNA 3′ processing before termination; Pta1 is an established
      core subunit. Complementation of defective budding yeast pta1 extracts establishes conserved participation
      in cleavage and poly(A) addition. The inference concerns complex participation, not intrinsic nuclease
      or polymerase activity.
    supported_by:
    - *id001
    - *id002
- term: &id003
    id: GO:0180010
    label: co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway
  evidence_type: IC
  original_reference_id: GO_REF:0000111
  qualifier: involved_in
  supporting_entities:
  - GO:0005849
  review:
    summary: co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway is supported.
    action: ACCEPT
    reason: CPF executes cotranscriptional mRNA 3′ processing before termination; Pta1 is an established
      core subunit. Complementation of defective budding yeast pta1 extracts establishes conserved participation
      in cleavage and poly(A) addition. The inference concerns complex participation, not intrinsic nuclease
      or polymerase activity.
    supported_by:
    - *id001
    - *id002
references:
- id: GO_REF:0000015
  title: Use of the ND evidence code for Gene Ontology (GO) terms
  findings: []
- id: GO_REF:0000024
  title: Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator
    judgment of sequence similarity
  findings: []
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings: []
- id: GO_REF:0000111
  title: Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity
    (ISS) annotation to support the inference
  findings: []
- id: GO_REF:0000120
  title: Combined Automated Annotation using Multiple IEA Methods
  findings: []
- id: PMID:14617822
  title: A comparative analysis of an orthologous proteomic environment in the yeasts Saccharomyces cerevisiae
    and Schizosaccharomyces pombe.
  findings: []
  full_text_unavailable: true
- id: PMID:16823372
  title: ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces
    pombe.
  findings: []
  full_text_unavailable: true
- id: PMID:33711009
  title: Structure-function analysis of fission yeast cleavage and polyadenylation factor (CPF) subunit
    Ppn1 and its interactions with Dis2 and Swd22.
  findings: []
  full_text_unavailable: false
- id: PMID:1508188
  title: PTA1, an essential gene of Saccharomyces cerevisiae affecting pre-tRNA processing.
  findings: []
  full_text_unavailable: true
- id: PMID:10523662
  title: Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA
    precursor.
  findings: []
  full_text_unavailable: false
core_functions:
- description: Noncatalytic CPF subunit organizing mRNA cleavage/polyadenylation and coupled transcription
    termination.
  supported_by:
  - *id001
  - *id002
  directly_involved_in:
  - *id003
  - *id004
  in_complex: *id005
  locations:
  - *id006