put1

UniProt ID: O74524
Organism: Schizosaccharomyces pombe 972h-
Review Status: COMPLETE
Aliases:
SPCC70.03c
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Gene Description

Put1 (SPCC70.03c) is the predicted mitochondrial FAD-dependent proline dehydrogenase of Schizosaccharomyces pombe. Conserved proline-oxidase architecture supports oxidation of L-proline to P5C with a quinone acceptor, initiating proline degradation toward glutamate. The catalytic assignment and mitochondrial targeting are primarily comparative. Fission-yeast isotope tracing establishes arginine conversion to proline and glutamate upstream of this pathway, but does not independently demonstrate an obligatory Put1-mediated proline shuttle.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0004657 proline dehydrogenase activity
IBA
GO_REF:0000033 		ACCEPT
Summary: PAINT IBA for proline dehydrogenase activity at node PTN004447157 (sources include S. cerevisiae PUT1, human PRODH/PRODH2, Arabidopsis ProDH1/2, Drosophila sluggish-A and bacterial PRODHs).
Reason: put1 belongs to the proline oxidase family (PTHR13914:SF0) and has the PRODH (betaalpha)8-barrel domain; the inherited activity is its core function.
Supporting Evidence:
file:SCHPO/put1/put1-uniprot.txt
Reaction=L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a
file:SCHPO/put1/put1-uniprot.txt
SIMILARITY: Belongs to the proline oxidase family.
GO:0004657 proline dehydrogenase activity
IEA
GO_REF:0000120 		ACCEPT
Summary: Combined IEA from ARBA00027069, InterPro IPR015659 (proline oxidase), Rhea 23784 and EC 1.5.5.2.
Reason: Correct activity (quinone as electron acceptor).
Supporting Evidence:
file:SCHPO/put1/put1-uniprot.txt
Reaction=L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a
GO:0005739 mitochondrion
IBA
GO_REF:0000033 		ACCEPT
Summary: PAINT IBA for mitochondrion at node PTN000357758 (sources include S. cerevisiae PUT1, Arabidopsis, Drosophila, mouse PRODH).
Reason: Eukaryotic PRODHs are mitochondrial and put1 has a predicted transit peptide.
Supporting Evidence:
file:SCHPO/put1/put1-uniprot.txt
SUBCELLULAR LOCATION: Mitochondrion.
GO:0005739 mitochondrion
IEA
GO_REF:0000044 		ACCEPT
Summary: UniProt SubCell mapping (SL-0173 Mitochondrion), assigned by sequence (transit peptide) rather than experiment.
Reason: Correct location.
Supporting Evidence:
file:SCHPO/put1/put1-uniprot.txt
SUBCELLULAR LOCATION: Mitochondrion.
GO:0005759 mitochondrial matrix
ISS
GO_REF:0000024 		ACCEPT
Summary: Matrix localization is transferred from curated budding-yeast Put1 in the fungal PRODH context.
Reason: Retain the source-specific ISS location as a core comparative assignment, rather than marking it peripheral solely because no direct S. pombe subfractionation assay was found. The donor has curated matrix localization and direct mitochondrial targeting; the exact membrane topology remains less well resolved.
Supporting Evidence:
file:SCHPO/put1/put1-uniprot.txt
SUBCELLULAR LOCATION: Mitochondrion.
file:SCHPO/put1/put1-uniprot.txt
Reaction=L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a
GO:0006562 L-proline catabolic process
IBA
GO_REF:0000033 		ACCEPT
Summary: PAINT IBA for L-proline catabolic process at node PTN004447157 (sources include S. cerevisiae PUT1, Candida PUT1, Drosophila, mouse, Arabidopsis).
Reason: Correct core process; matches the PomBase GO-CAM 678073a900003902.
Supporting Evidence:
file:SCHPO/put1/put1-uniprot.txt
FUNCTION: Converts proline to delta-1-pyrroline-5-carboxylate.
GO:0006562 L-proline catabolic process
IEA
GO_REF:0000002 		ACCEPT
Summary: InterPro2GO from IPR015659 (proline oxidase) to L-proline catabolic process.
Reason: Correct core process.
Supporting Evidence:
file:SCHPO/put1/put1-uniprot.txt
FUNCTION: Converts proline to delta-1-pyrroline-5-carboxylate.
GO:0071949 FAD binding
IBA
GO_REF:0000033 		ACCEPT
Summary: PAINT IBA for FAD binding at node PTN000357758 (source human PRODH).
Reason: FAD is the catalytic cofactor of PRODH; consistent with the yeast PUT1 review.
Supporting Evidence:
file:SCHPO/put1/put1-uniprot.txt
Name=FAD; Xref=ChEBI:CHEBI:57692;

Core Functions

Predicted mitochondrial FAD-dependent proline oxidation to P5C using a quinone electron acceptor, the first proline-degradation reaction. The function is supported by the inherited PRODH clade and curated domains; obligatory participation in a native arginine proline shuttle is not directly established.

Molecular Function:
proline dehydrogenase activity
Directly Involved In:
L-proline catabolic process
Cellular Locations:
mitochondrion
Supporting Evidence:
  • file:SCHPO/put1/put1-uniprot.txt
    Reaction=L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a
  • file:SCHPO/put1/put1-uniprot.txt
    FUNCTION: Converts proline to delta-1-pyrroline-5-carboxylate.
  • file:SCHPO/put1/put1-uniprot.txt
    Name=FAD; Xref=ChEBI:CHEBI:57692;
  • file:SCHPO/put1/put1-uniprot.txt
    SUBCELLULAR LOCATION: Mitochondrion.

References

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Suggested Questions for Experts

Q: UniProt O74524 carries no gene name (only SPCC70.03c), so symbol-based lookups for put1 fail; could the PomBase name be propagated to UniProt?

Suggested Experiments

Experiment: Test growth of a put1 deletion on L-proline as sole nitrogen source and measure proline dehydrogenase activity in isolated mitochondria.

📚 Additional Documentation

Notes

(put1-notes.md)

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