| Claim category | Specific claim | Evidence source (paper) | Pub year | DOI/URL | Evidence snippet (paraphrased) | Confidence |
|---|---|---|---|---|---|---|
| identity | The target in the literature is **Schizosaccharomyces pombe ral2**, a Ras1-pathway regulatory gene/product, not a Ras-family small GTPase. | Imai et al., *Mol Cell Biol*; Hakuno et al., *Genes Cells*; Kawamukai, *Biosci Biotechnol Biochem* | 1991; 1996; 2024 | https://doi.org/10.1128/mcb.11.6.3088-3094.1991 ; https://doi.org/10.1046/j.1365-2443.1996.27029.x ; https://doi.org/10.1093/bbb/zbae019 | Multiple sources describe ral2 as a “putative activator of Ras1” or “Ras1-Scd pathway protein,” distinguishing it from ras1 itself. (pqac-00000008, pqac-00000009, pqac-00000010, pqac-00000011, pqac-00000012) | High |
| domains | S. pombe Ral2 is reported to contain **N-terminal kelch repeats**. | Qu et al., *Front Plant Sci* | 2021 | https://doi.org/10.3389/fpls.2021.702368 | Comparative discussion of S. pombe Ral2 states it has three kelch repeats at the N-terminus and belongs to the Ras1-Scd pathway context. (pqac-00000000, pqac-00000003, pqac-00000005) | Medium |
| domains | Older kelch-family phylogeny lists S. pombe Ral-2 as a **kelch-repeat protein** with “Kelch and unique” architecture, not as a canonical BTB/kelch protein. | Prag & Adams, *BMC Bioinformatics* | 2003 | https://doi.org/10.1186/1471-2105-4-42 | Table-based annotation places S. pombe Ral-2 among kelch-repeat proteins and notes yeasts lacked BTB/kelch proteins in that analysis. (pqac-00000004) | Medium |
| domains | **Direct support for a BTB/POZ domain in S. pombe Ral2 is not established by the gathered primary evidence**; BTB is explicit for the Pyricularia homolog, not unequivocally for S. pombe in these texts. | Qu et al., *Front Plant Sci*; Prag & Adams, *BMC Bioinformatics* | 2021; 2003 | https://doi.org/10.3389/fpls.2021.702368 ; https://doi.org/10.1186/1471-2105-4-42 | The fungal homolog PoRal2 is described with a C-terminal BTB domain, but the gathered S. pombe-focused evidence only clearly supports kelch repeats and Ras1-pathway assignment. (pqac-00000002, pqac-00000003, pqac-00000004, pqac-00000005) | Medium |
| function | ral2 likely functions as a **positive regulator/activator of Ras1**. | Imai et al., *Mol Cell Biol*; Hakuno et al., *Genes Cells* | 1991; 1996 | https://doi.org/10.1128/mcb.11.6.3088-3094.1991 ; https://doi.org/10.1046/j.1365-2443.1996.27029.x | Genetic studies repeatedly frame ral2 as a putative Ras1 activator; the mechanism remained unresolved. (pqac-00000001, pqac-00000007, pqac-00000008, pqac-00000009, pqac-00000010, pqac-00000011) | High |
| pathway | ral2 acts in the **Ras1-Scd signaling pathway** tied to mating and morphogenesis. | Qu et al., *Front Plant Sci*; Kawamukai, *Biosci Biotechnol Biochem* | 2021; 2024 | https://doi.org/10.3389/fpls.2021.702368 ; https://doi.org/10.1093/bbb/zbae019 | Recent review and comparative paper both label ral2 as a Ras1-Scd pathway protein. (pqac-00000002, pqac-00000003, pqac-00000005, pqac-00000012) | High |
| pathway | Genetic evidence places ral2 **upstream of or affecting Ras1 activation**, with intimate linkage to the Ras-GTPase cycle and interaction logic involving **Gap1**. | Imai et al., *Mol Cell Biol* | 1991 | https://doi.org/10.1128/mcb.11.6.3088-3094.1991 | Suppressor and epistasis analyses showed gap1 null could bypass ral2 requirement for maintaining Ras1-GTP, supporting a role in Ras1 activation control. (pqac-00000008, pqac-00000009, pqac-00000010) | High |
| phenotype | ral2 mutants are associated with **mating deficiency**. | Hakuno et al., *Genes Cells*; Imai et al., *Mol Cell Biol* | 1996; 1991 | https://doi.org/10.1046/j.1365-2443.1996.27029.x ; https://doi.org/10.1128/mcb.11.6.3088-3094.1991 | ral genes, including ral2, were cloned from mutants defective in mating; loss of ral2 gives phenotypes similar to reduced Ras1 signaling. (pqac-00000007, pqac-00000009) | High |
| phenotype | ral2 mutants show **roundish/abnormal cell morphology**, linking ral2 to polarized growth control. | Hakuno et al., *Genes Cells* | 1996 | https://doi.org/10.1046/j.1365-2443.1996.27029.x | The ral mutant class is described as mating-deficient with roundish morphology, and ral-linked signaling is tied to Ras1-dependent morphology regulation. (pqac-00000001, pqac-00000007) | High |
| phenotype | Earlier comparative discussion also links S. pombe ral2 to **control of cell morphology, conjugation/mating, and sporulation**. | Qu et al., *Front Plant Sci* | 2021 | https://doi.org/10.3389/fpls.2021.702368 | Review-style summary of prior S. pombe literature states Ral2 participates in morphology, conjugation, and sporulation; this is secondary reporting of older work. (pqac-00000000) | Medium |
| interactions | Evidence from gathered texts supports a functional/genetic relationship between **Ral2 and Ras1**, and between **Ral2 and Gap1**. | Imai et al., *Mol Cell Biol* | 1991 | https://doi.org/10.1128/mcb.11.6.3088-3094.1991 | ras1 activating mutations suppress ral2 defects, and gap1 epistasis indicates ral2 operates in the Ras1 regulatory cycle. (pqac-00000008, pqac-00000009, pqac-00000010) | High |
| interactions | Secondary reporting indicates S. pombe Ral2 has been reported to interact with **Gef1** and **Skp1**, but these claims were not directly verified here from the original S. pombe primary papers. | Qu et al., *Front Plant Sci* | 2021 | https://doi.org/10.3389/fpls.2021.702368 | Comparative fungal paper summarizes earlier S. pombe work saying Ral2 interacts with Gef1 and Skp1; this remains indirect evidence in the present retrieval set. (pqac-00000000, pqac-00000002) | Low |
| notes | Mechanistically, ral2 was hypothesized to act either as a **GDP-GTP exchange-type activator** for Ras1 or as an **indirect negative regulator of Gap1**, but the exact biochemical mechanism remained unresolved. | Imai et al., *Mol Cell Biol* | 1991 | https://doi.org/10.1128/mcb.11.6.3088-3094.1991 | Authors explicitly discuss alternative mechanistic models for how ral2 promotes Ras1 activity. (pqac-00000009, pqac-00000010) | High |
| notes | **Direct primary paper not retrieved here:** Fukui et al. 1989 is repeatedly cited as the original ral2 characterization, but only secondary references to it were available in the current evidence set. | Fukui et al., *Mol Cell Biol* (cited as unobtainable in search results) | 1989 | https://doi.org/10.1128/mcb.9.12.5617-5622.1989 | The foundational paper on ral2 characterization was identified but not retrievable in the current tool output; conclusions attributed to it here are therefore partly mediated through later sources. (pqac-00000000, pqac-00000001, pqac-00000011) | High |


*Table: This table consolidates the gathered evidence for S. pombe ral2/SPBC21.05c (UniProt P15258), separating direct support from indirect or secondary claims. It is useful for distinguishing well-supported conclusions about Ras1-pathway function and phenotype from less certain domain and interaction annotations.*