Rpo41 is the mitochondrial DNA-directed RNA polymerase of Schizosaccharomyces pombe. It binds mitochondrial promoters and synthesizes RNA from DNA templates; association with the transcription factor Mtf1 greatly enhances promoter-directed initiation. Its transcriptional activity sustains mitochondrial gene expression.
Existing Annotations Review
GO Term
Evidence
Action
Reason
GO:0001018 mitochondrial promoter sequence-specific DNA binding
EXP PMID:21357609 Identification and characterization of the mitochondrial RNA...
ACCEPT
Summary: mitochondrial promoter sequence-specific DNA binding is supported.
Reason: Purified fission-yeast Rpo41 binds mitochondrial promoters in electrophoretic mobility-shift assays, including activity without Mtf1; Mtf1 enhances productive promoter recognition.
We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro.
GO:0001018 mitochondrial promoter sequence-specific DNA binding
IBA GO_REF:0000033
ACCEPT
Summary: mitochondrial promoter sequence-specific DNA binding is supported.
Reason: Purified fission-yeast Rpo41 binds mitochondrial promoters in electrophoretic mobility-shift assays, including activity without Mtf1; Mtf1 enhances productive promoter recognition.
We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro.
Summary: DNA binding is reviewed in the context of rpo41.
Reason: The broad DNA binding annotation is compatible with the biology, but mitochondrial promoter sequence-specific DNA binding captures the supported rpo41 function more precisely.
We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro.
EXP PMID:21357609 Identification and characterization of the mitochondrial RNA...
ACCEPT
Summary: DNA-directed RNA polymerase activity is supported.
Reason: Reconstituted transcription with purified fission-yeast Rpo41 directly establishes DNA-directed RNA synthesis. This is an intrinsic catalytic polymerase, with Mtf1 enhancing initiation.
We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro.
Summary: DNA-directed RNA polymerase activity is supported.
Reason: Reconstituted transcription with purified fission-yeast Rpo41 directly establishes DNA-directed RNA synthesis. This is an intrinsic catalytic polymerase, with Mtf1 enhancing initiation.
We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro.
Summary: DNA-directed RNA polymerase activity is supported.
Reason: Reconstituted transcription with purified fission-yeast Rpo41 directly establishes DNA-directed RNA synthesis. This is an intrinsic catalytic polymerase, with Mtf1 enhancing initiation.
We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro.
Reason: Matrix residence is a curator-mediated similarity inference consistent with access to mitochondrial DNA and the conserved mitochondrial transcription machinery. No target-specific divergence is evident.
We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro.
Summary: DNA-templated transcription is reviewed in the context of rpo41.
Reason: The broad DNA-templated transcription annotation is compatible with the biology, but mitochondrial transcription captures the supported rpo41 function more precisely.
We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro.
We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro.
We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro.
GO:0006391 transcription initiation at mitochondrial promoter
IMP PMID:21357609 Identification and characterization of the mitochondrial RNA...
ACCEPT
Summary: transcription initiation at mitochondrial promoter is supported.
Reason: The reconstituted system initiates at mitochondrial promoters and recapitulates the in-vivo start sites.
We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro.
Summary: 5'-3' RNA polymerase activity is supported.
Reason: The direct DNA-templated RNA synthesis assay supports this broader directional RNA polymerization activity; it is compatible with the specific DNA-directed polymerase annotation.
We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro.
Summary: mitochondrial DNA-directed RNA polymerase complex is supported.
Reason: Mtf1 and Rpo41 form the functional mitochondrial transcription machinery; purified factors bind promoters more efficiently together and support transcription.
We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro.
IDA PMID:21357609 Identification and characterization of the mitochondrial RNA...
ACCEPT
Summary: mitochondrial DNA-directed RNA polymerase complex is supported.
Reason: Mtf1 and Rpo41 form the functional mitochondrial transcription machinery; purified factors bind promoters more efficiently together and support transcription.
We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro.
Summary: mitochondrial DNA-directed RNA polymerase complex is supported.
Reason: Mtf1 and Rpo41 form the functional mitochondrial transcription machinery; purified factors bind promoters more efficiently together and support transcription.
We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro.
Core Functions
Catalyzes mitochondrial promoter-directed RNA synthesis in cooperation with Mtf1.
We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro.
These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.
Review rationale: Purified S. pombe Rpo41 binds mitochondrial promoters and supports transcription together with Mtf1 (PMID:21357609). The predicted DNA binding is biologically supported. The existing mitochondrial promoter sequence-specific DNA binding annotation is more specific within the same GO aspect, so the prediction is LSP.
Supporting Evidence:
PMID:21357609: "We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro."
Review rationale: Purified S. pombe Rpo41 binds mitochondrial promoters and supports transcription together with Mtf1 (PMID:21357609). The predicted DNA-templated transcription is biologically supported. The existing mitochondrial transcription annotation is more specific within the same GO aspect, so the prediction is LSP.
Supporting Evidence:
PMID:21357609: "We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro."
Review rationale: Purified S. pombe Rpo41 binds mitochondrial promoters and supports transcription together with Mtf1 (PMID:21357609). The predicted DNA-directed RNA polymerase complex is biologically supported. The existing mitochondrial DNA-directed RNA polymerase complex annotation is more specific within the same GO aspect, so the prediction is LSP.
Supporting Evidence:
PMID:21357609: "We show biochemically, that purified Mtf1 and Rpo41 together can bind to the S. pombe mitochondrial promoters and can support transcription from the S. pombe mitochondrial promoters in vitro."