id: Q9P6P2
gene_symbol: rrp36
product_type: PROTEIN
status: COMPLETE
taxon:
  id: NCBITaxon:284812
  label: Schizosaccharomyces pombe (strain 972 / ATCC 24843)
description: Rrp36 is a conserved nucleolar ribosome-biogenesis factor associated with the 90S preribosome.
  It supports early processing of the small-subunit rRNA precursor and production of the 40S ribosomal
  subunit. Comparative experiments in budding yeast and human cells establish a conserved requirement
  for early pre-rRNA cleavages, while fission yeast localization and curated phylogenetic evidence support
  the corresponding role of Rrp36. An intrinsic RNA-cleaving activity has not been established.
existing_annotations:
- term: &id003
    id: GO:0000462
    label: maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: involved_in
  supporting_entities:
  - PANTHER:PTN000497661
  - SGD:S000005813
  review:
    summary: maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
      is supported.
    action: ACCEPT
    reason: The target has the specific RRP36 family domain and a nucleolar localization in the fission
      yeast screen. Budding yeast purification and depletion experiments establish 90S/pre-40S association
      and early SSU-rRNA maturation, and human depletion supports conservation across eukaryotes. This
      agrees with the curated ancestral assertion and justifies transfer to the fission yeast ortholog.
    supported_by:
    - &id001
      reference_id: PMID:20038530
      supporting_text: We show that the Rrp36p protein is nucleolar and interacts with 90S and pre-40S
        preribosomal particles. Its depletion affects early cleavages of the 35S pre-rRNA and results
        in a rapid decrease in mature 18S rRNA levels.
    - &id002
      reference_id: PMID:16823372
      supporting_text: Next, we determined the localization of 4,431 proteins, corresponding to approximately
        90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein.
- term:
    id: GO:0003674
    label: molecular_function
  evidence_type: ND
  original_reference_id: GO_REF:0000015
  qualifier: enables
  review:
    summary: molecular_function is supported.
    action: ACCEPT
    reason: Rrp36 participates in preribosome assembly and processing, but the reviewed experiments do
      not establish an intrinsic enzymatic or specific binding activity. Retain the ND molecular-function
      root instead of inventing an rRNA nuclease activity.
    supported_by:
    - *id001
- term:
    id: GO:0005634
    label: nucleus
  evidence_type: HDA
  original_reference_id: PMID:16823372
  qualifier: is_active_in
  review:
    summary: nucleus is supported.
    action: ACCEPT
    reason: The target has the specific RRP36 family domain and a nucleolar localization in the fission
      yeast screen. Budding yeast purification and depletion experiments establish 90S/pre-40S association
      and early SSU-rRNA maturation, and human depletion supports conservation across eukaryotes. This
      agrees with the curated ancestral assertion and justifies transfer to the fission yeast ortholog.
    supported_by:
    - *id001
    - *id002
- term: &id005
    id: GO:0005730
    label: nucleolus
  evidence_type: HDA
  original_reference_id: PMID:16823372
  qualifier: is_active_in
  review:
    summary: nucleolus is supported.
    action: ACCEPT
    reason: The target has the specific RRP36 family domain and a nucleolar localization in the fission
      yeast screen. Budding yeast purification and depletion experiments establish 90S/pre-40S association
      and early SSU-rRNA maturation, and human depletion supports conservation across eukaryotes. This
      agrees with the curated ancestral assertion and justifies transfer to the fission yeast ortholog.
    supported_by:
    - *id001
    - *id002
- term:
    id: GO:0005730
    label: nucleolus
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: is_active_in
  supporting_entities:
  - PANTHER:PTN000497661
  - SGD:S000005813
  - UniProtKB:Q96EU6
  review:
    summary: nucleolus is supported.
    action: ACCEPT
    reason: The target has the specific RRP36 family domain and a nucleolar localization in the fission
      yeast screen. Budding yeast purification and depletion experiments establish 90S/pre-40S association
      and early SSU-rRNA maturation, and human depletion supports conservation across eukaryotes. This
      agrees with the curated ancestral assertion and justifies transfer to the fission yeast ortholog.
    supported_by:
    - *id001
    - *id002
- term:
    id: GO:0005730
    label: nucleolus
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  supporting_entities:
  - UniProtKB-SubCell:SL-0188
  review:
    summary: nucleolus is supported.
    action: ACCEPT
    reason: The target has the specific RRP36 family domain and a nucleolar localization in the fission
      yeast screen. Budding yeast purification and depletion experiments establish 90S/pre-40S association
      and early SSU-rRNA maturation, and human depletion supports conservation across eukaryotes. This
      agrees with the curated ancestral assertion and justifies transfer to the fission yeast ortholog.
    supported_by:
    - *id001
    - *id002
- term: &id004
    id: GO:0030686
    label: 90S preribosome
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: part_of
  supporting_entities:
  - PANTHER:PTN000497661
  - SGD:S000005813
  review:
    summary: 90S preribosome is supported.
    action: ACCEPT
    reason: The target has the specific RRP36 family domain and a nucleolar localization in the fission
      yeast screen. Budding yeast purification and depletion experiments establish 90S/pre-40S association
      and early SSU-rRNA maturation, and human depletion supports conservation across eukaryotes. This
      agrees with the curated ancestral assertion and justifies transfer to the fission yeast ortholog.
    supported_by:
    - *id001
    - *id002
references:
- id: GO_REF:0000015
  title: Use of the ND evidence code for Gene Ontology (GO) terms
  findings: []
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings: []
- id: GO_REF:0000044
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping,
    accompanied by conservative changes to GO terms applied by UniProt
  findings: []
- id: PMID:16823372
  title: ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces
    pombe.
  findings: []
  full_text_unavailable: true
- id: PMID:20038530
  title: Evolutionarily conserved function of RRP36 in early cleavages of the pre-rRNA and production
    of the 40S ribosomal subunit.
  findings: []
  full_text_unavailable: true
core_functions:
- description: Nucleolar 90S preribosome factor supporting early small-subunit rRNA maturation; function
    transferred from characterized RRP36 orthologs.
  supported_by:
  - *id001
  directly_involved_in:
  - *id003
  in_complex: *id004
  locations:
  - *id005