sdh1 (SPAC1556.02c; UniProt Q9UTJ7) encodes the flavoprotein (SdhA) subunit, carrying covalent FAD and the succinate-binding site, of mitochondrial succinate dehydrogenase (respiratory complex II), which oxidises succinate to fumarate in the TCA cycle and passes the electrons through FAD and Fe-S clusters to ubiquinone in the inner membrane. Function is assigned by orthology to the S. cerevisiae SDH subunits.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005739 mitochondrion | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: ORFeome YFP screen: mitochondrion. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0005739 mitochondrion | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference of mitochondrial location. Reason: By similarity: UniProt places the protein in the mitochondrial inner membrane on the strength of the S. cerevisiae SDH1 ortholog (Q00711), and the protein is a member of the mitochondrial SdhA subfamily (TIGR01816). The PAINT node therefore sits correctly in the eukaryotic mitochondrial SdhA clade; the root organelle claim holds and the inner-membrane matrix-side location is carried by separate rows. Supporting Evidence: file:SCHPO/sdh1/sdh1-uniprot.txt Mitochondrion inner membrane {ECO:0000250|UniProtKB:Q00711}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q00711}; Matrix side file:SCHPO/sdh1/sdh1-uniprot.txt NCBIfam; TIGR01816; sdhA_forward; 1. |
| GO:0005743 mitochondrial inner membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Complex II sits in the inner mitochondrial membrane. Reason: By similarity to S. cerevisiae SDH1 (Q00711): curated UniProt location is the inner mitochondrial membrane as a matrix-side peripheral protein, consistent with its role as the flavoprotein subunit of membrane-anchored complex II. Supporting Evidence: file:SCHPO/sdh1/sdh1-uniprot.txt Mitochondrion inner membrane {ECO:0000250|UniProtKB:Q00711}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q00711}; Matrix side file:SCHPO/sdh1/sdh1-uniprot.txt Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit. |
| GO:0005743 mitochondrial inner membrane | ISS GO_REF:0000024 | ACCEPT | Summary: Complex II sits in the inner mitochondrial membrane. Reason: By similarity to S. cerevisiae SDH1 (Q00711): curated UniProt location is the inner mitochondrial membrane as a matrix-side peripheral protein, consistent with its role as the flavoprotein subunit of membrane-anchored complex II. Supporting Evidence: file:SCHPO/sdh1/sdh1-uniprot.txt Mitochondrion inner membrane {ECO:0000250|UniProtKB:Q00711}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q00711}; Matrix side file:SCHPO/sdh1/sdh1-uniprot.txt Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit. |
| GO:0006099 tricarboxylic acid cycle | IEA GO_REF:0000120 | ACCEPT | Summary: InterPro/UniPathway mapping; consistent. Reason: By similarity to the SdhA orthologs (P31040): the curated UniProt pathway places the protein in the succinate-to-fumarate step of the TCA cycle, and the catalysed reaction (succinate to fumarate) is that step. Supporting Evidence: file:SCHPO/sdh1/sdh1-uniprot.txt Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1. file:SCHPO/sdh1/sdh1-uniprot.txt Reaction=a quinone + succinate = fumarate + a quinol; |
| GO:0006099 tricarboxylic acid cycle | ISO GO_REF:0000024 | ACCEPT | Summary: Transfer from the S. cerevisiae ortholog. Reason: By similarity to the SdhA orthologs (P31040): the curated UniProt pathway places the protein in the succinate-to-fumarate step of the TCA cycle, and the catalysed reaction (succinate to fumarate) is that step. Supporting Evidence: file:SCHPO/sdh1/sdh1-uniprot.txt Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1. file:SCHPO/sdh1/sdh1-uniprot.txt Reaction=a quinone + succinate = fumarate + a quinol; |
| GO:0006121 mitochondrial electron transport, succinate to ubiquinone | IBA GO_REF:0000033 | ACCEPT | Summary: Succinate-to-ubiquinone electron transport by complex II. Reason: By similarity (ECO:0000250) to characterised SdhA orthologs: the curated FUNCTION states that the flavoprotein subunit of complex II transfers electrons from succinate to ubiquinone. The PAINT node is within the SdhA clade (TIGR01816), so the IBA is sound. Supporting Evidence: file:SCHPO/sdh1/sdh1-uniprot.txt Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). file:SCHPO/sdh1/sdh1-uniprot.txt NCBIfam; TIGR01816; sdhA_forward; 1. |
| GO:0006121 mitochondrial electron transport, succinate to ubiquinone | ISS GO_REF:0000024 | ACCEPT | Summary: Succinate-to-ubiquinone electron transport by complex II. Reason: By similarity (ECO:0000250) to characterised SdhA orthologs: the curated FUNCTION states that the flavoprotein subunit of complex II transfers electrons from succinate to ubiquinone. Supporting Evidence: file:SCHPO/sdh1/sdh1-uniprot.txt Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). |
| GO:0008177 succinate dehydrogenase (quinone) activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference: contributes to succinate dehydrogenase (quinone) activity as a complex II subunit. Reason: By similarity to human SDHA (P31040): the curated catalytic activity is the succinate:quinone oxidoreductase reaction, and the flavoprotein is one of four complex II subunits, so contributes_to is the right qualifier. The PAINT node lies in the SdhA/FrdA family (PANTHER PTHR11632). Supporting Evidence: file:SCHPO/sdh1/sdh1-uniprot.txt Reaction=a quinone + succinate = fumarate + a quinol; file:SCHPO/sdh1/sdh1-uniprot.txt Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit. |
| GO:0008177 succinate dehydrogenase (quinone) activity | IEA GO_REF:0000120 | ACCEPT | Summary: EC/InterPro mapping; consistent. Reason: By similarity to human SDHA (P31040) and family membership: the curated catalytic activity is EC 1.3.5.1 (succinate + quinone = fumarate + quinol), and the protein carries the forward-direction SdhA signature (IPR011281). Supporting Evidence: file:SCHPO/sdh1/sdh1-uniprot.txt Reaction=a quinone + succinate = fumarate + a quinol; file:SCHPO/sdh1/sdh1-uniprot.txt InterPro; IPR011281; Succ_DH_flav_su_fwd. |
| GO:0008177 succinate dehydrogenase (quinone) activity | ISS GO_REF:0000024 | ACCEPT | Summary: Similarity transfer; consistent. Reason: By similarity to human SDHA (P31040): the curated catalytic activity is the succinate:quinone oxidoreductase reaction catalysed by complex II, of which this is the flavoprotein subunit. Supporting Evidence: file:SCHPO/sdh1/sdh1-uniprot.txt Reaction=a quinone + succinate = fumarate + a quinol; file:SCHPO/sdh1/sdh1-uniprot.txt Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). |
| GO:0016020 membrane | IEA GO_REF:0000117 | TOO GENERAL | Summary: Generic membrane; inner membrane is annotated. Reason: True but uninformative: by similarity to S. cerevisiae SDH1 (Q00711) the protein is a matrix-side peripheral protein of the mitochondrial inner membrane, which is annotated on its own rows and entails membrane. Supporting Evidence: file:SCHPO/sdh1/sdh1-uniprot.txt Mitochondrion inner membrane {ECO:0000250|UniProtKB:Q00711}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q00711}; Matrix side |
| GO:0016491 oxidoreductase activity | IEA GO_REF:0000002 | TOO GENERAL | Summary: Generic oxidoreductase; succinate dehydrogenase activity is annotated. Reason: True but uninformative: by similarity to human SDHA (P31040) the protein catalyses the succinate:quinone oxidoreductase reaction, annotated as GO:0008177, which entails oxidoreductase activity. Supporting Evidence: file:SCHPO/sdh1/sdh1-uniprot.txt Reaction=a quinone + succinate = fumarate + a quinol; |
| GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors | IEA GO_REF:0000002 | TOO GENERAL | Summary: Generic CH-CH oxidoreductase; succinate dehydrogenase activity is annotated. Reason: True but uninformative: by similarity to human SDHA (P31040) the protein oxidises the CH-CH bond of succinate to fumarate with quinone as acceptor, annotated as GO:0008177, which entails this term. Supporting Evidence: file:SCHPO/sdh1/sdh1-uniprot.txt Reaction=a quinone + succinate = fumarate + a quinol; |
| GO:0022900 electron transport chain | IEA GO_REF:0000002 | TOO GENERAL | Summary: Broad parent. Reason: True but uninformative: by similarity, the curated FUNCTION places the protein in complex II of the mitochondrial electron transport chain, annotated more specifically as GO:0006121 succinate-to-ubiquinone electron transport. Supporting Evidence: file:SCHPO/sdh1/sdh1-uniprot.txt Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). |
| GO:0045273 respiratory chain complex II (succinate dehydrogenase) | IBA GO_REF:0000033 | ACCEPT | Summary: Subunit of respiratory complex II (SDH). Reason: By similarity to S. cerevisiae SDH1 (Q00711): the curated SUBUNIT line describes the four-subunit complex II of which this protein is the flavoprotein. The PAINT node lies in the SdhA clade (TIGR01816), so the IBA is sound. Supporting Evidence: file:SCHPO/sdh1/sdh1-uniprot.txt Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit. file:SCHPO/sdh1/sdh1-uniprot.txt Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). |
| GO:0050660 flavin adenine dinucleotide binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: Covalent FAD cofactor of the flavoprotein; subsidiary. Reason: By similarity to the chicken SdhA structure (Q9YHT1) and family membership: FAD is the curated cofactor, it is attached covalently through a predicted 8alpha-FAD histidine, and the protein carries the FRD/SDH FAD-binding PROSITE signature. FAD binding is true but is the cofactor of the core succinate dehydrogenase activity, so non-core. Supporting Evidence: file:SCHPO/sdh1/sdh1-uniprot.txt Name=FAD; Xref=ChEBI:CHEBI:57692; file:SCHPO/sdh1/sdh1-uniprot.txt Tele-8alpha-FAD histidine file:SCHPO/sdh1/sdh1-uniprot.txt PROSITE; PS00504; FRD_SDH_FAD_BINDING; 1. |
| GO:0099617 matrix side of mitochondrial inner membrane | IC GO_REF:0000111 | ACCEPT | Summary: The SDH catalytic subunits face the matrix side of the inner membrane. Reason: By similarity to S. cerevisiae SDH1 (Q00711): the curated location is the inner membrane, peripheral, on the matrix side, which is where the succinate-oxidising flavoprotein acts. Supporting Evidence: file:SCHPO/sdh1/sdh1-uniprot.txt Mitochondrion inner membrane {ECO:0000250|UniProtKB:Q00711}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q00711}; Matrix side |
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