Sec59 is an endoplasmic-reticulum membrane dolichol kinase that produces dolichyl phosphate by CTP-dependent phosphorylation of dolichol. Dolichyl phosphate supplies the lipid carrier needed for protein N-glycosylation, O-mannosylation and GPI-anchor formation. Conserved Sec59/DOLK orthology supports this function in fission yeast; the related CTP-dependent phosphorylation of diacylglycerol is a distinct enzyme activity.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004168 dolichol kinase activity | IBA GO_REF:0000033 | ACCEPT | Summary: Sec59 catalyzes CTP-dependent dolichol phosphorylation. Reason: The target’s curated transfer identifies experimentally characterized budding-yeast Sec59, and its full-length membrane-kinase architecture is consistent. The biochemical donor studies distinguish dolichol kinase from DAG kinase. This supports the orthology-based assignment independently of ARBA. Supporting Evidence: file:SCHPO/sec59/sec59-uniprot.txt CC -!- FUNCTION: Catalyzes CTP-mediated phosphorylation of dolichol, the PMID:12213788 Dolichol kinase (DK) catalyzes the CTP-mediated phosphorylation of dolichol in eukaryotic cells, the terminal step in dolichyl monophosphate (Dol-P) biosynthesis de novo. |
| GO:0004168 dolichol kinase activity | IEA GO_REF:0000120 | ACCEPT | Summary: Sec59 catalyzes CTP-dependent dolichol phosphorylation. Reason: The target’s curated transfer identifies experimentally characterized budding-yeast Sec59, and its full-length membrane-kinase architecture is consistent. The biochemical donor studies distinguish dolichol kinase from DAG kinase. This supports the orthology-based assignment independently of ARBA. Supporting Evidence: file:SCHPO/sec59/sec59-uniprot.txt CC -!- FUNCTION: Catalyzes CTP-mediated phosphorylation of dolichol, the PMID:12213788 Dolichol kinase (DK) catalyzes the CTP-mediated phosphorylation of dolichol in eukaryotic cells, the terminal step in dolichyl monophosphate (Dol-P) biosynthesis de novo. |
| GO:0004168 dolichol kinase activity | ISS GO_REF:0000024 | ACCEPT | Summary: Sec59 catalyzes CTP-dependent dolichol phosphorylation. Reason: The target’s curated transfer identifies experimentally characterized budding-yeast Sec59, and its full-length membrane-kinase architecture is consistent. The biochemical donor studies distinguish dolichol kinase from DAG kinase. This supports the orthology-based assignment independently of ARBA. Supporting Evidence: file:SCHPO/sec59/sec59-uniprot.txt CC -!- FUNCTION: Catalyzes CTP-mediated phosphorylation of dolichol, the PMID:12213788 Dolichol kinase (DK) catalyzes the CTP-mediated phosphorylation of dolichol in eukaryotic cells, the terminal step in dolichyl monophosphate (Dol-P) biosynthesis de novo. |
| GO:0005783 endoplasmic reticulum | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | MODIFY | Summary: Sec59 acts in the ER membrane. Reason: The target localization observation and multi-pass membrane topology support the membrane-specific location. Proposed replacements: endoplasmic reticulum membrane Supporting Evidence: file:SCHPO/sec59/sec59-uniprot.txt CC -!- FUNCTION: Catalyzes CTP-mediated phosphorylation of dolichol, the |
| GO:0005789 endoplasmic reticulum membrane | IBA GO_REF:0000033 | ACCEPT | Summary: Sec59 is an ER membrane kinase. Reason: Conserved membrane topology and the target ER localization support the compartment; no claim of a soluble kinase is needed. Supporting Evidence: file:SCHPO/sec59/sec59-uniprot.txt CC -!- FUNCTION: Catalyzes CTP-mediated phosphorylation of dolichol, the |
| GO:0005789 endoplasmic reticulum membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Sec59 is an ER membrane kinase. Reason: Conserved membrane topology and the target ER localization support the compartment; no claim of a soluble kinase is needed. Supporting Evidence: file:SCHPO/sec59/sec59-uniprot.txt CC -!- FUNCTION: Catalyzes CTP-mediated phosphorylation of dolichol, the |
| GO:0043048 dolichyl monophosphate biosynthetic process | IBA GO_REF:0000033 | ACCEPT | Summary: Sec59 produces dolichyl monophosphate. Reason: Dolichol phosphorylation is the terminal biosynthetic step furnishing the shared lipid carrier. Supporting Evidence: PMID:12213788 Dolichol kinase (DK) catalyzes the CTP-mediated phosphorylation of dolichol in eukaryotic cells, the terminal step in dolichyl monophosphate (Dol-P) biosynthesis de novo. file:SCHPO/sec59/sec59-uniprot.txt CC -!- FUNCTION: Catalyzes CTP-mediated phosphorylation of dolichol, the |
| GO:0180047 dolichol phosphate mannose biosynthetic process | IC GO_REF:0000111 | KEEP AS NON CORE | Summary: Sec59 supplies dolichyl phosphate for downstream dolichyl-phosphate-mannose synthesis. Reason: The IC process annotation is valid pathway participation through precursor supply; Sec59 does not itself transfer mannose, and the direct core process is dolichyl phosphate synthesis. Supporting Evidence: PMID:12213788 Dolichol kinase (DK) catalyzes the CTP-mediated phosphorylation of dolichol in eukaryotic cells, the terminal step in dolichyl monophosphate (Dol-P) biosynthesis de novo. |
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