id: Q7LKV3
gene_symbol: sen15
product_type: PROTEIN
status: COMPLETE
taxon:
  id: NCBITaxon:284812
  label: Schizosaccharomyces pombe (strain 972 / ATCC 24843)
description: Sen15 is a small, conserved noncatalytic subunit of the heterotetrameric tRNA-splicing endonuclease
  complex. Together with Sen2, Sen34 and Sen54 it supports recognition and excision of introns from precursor
  tRNAs before the cleaved exons are ligated. The catalytic splice-site cleavage centers reside in Sen2
  and Sen34. Nuclear and cytosolic pools have been reported in fission yeast localization screens.
existing_annotations:
- term: &id004
    id: GO:0000214
    label: tRNA-intron endonuclease complex
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: part_of
  supporting_entities:
  - PANTHER:PTN002003396
  - SGD:S000004663
  review:
    summary: tRNA-intron endonuclease complex is supported.
    action: ACCEPT
    reason: The target is a SEN15-family protein, supporting transfer of the characterized four-subunit
      tRNA-splicing endonuclease role. SEN2 and SEN34 encode the cleavage active sites; Sen15 is a noncatalytic
      participant in intron recognition/excision. Participation in the overall cleavage-and-ligation pathway
      does not assert that Sen15 ligates RNA.
    supported_by:
    - &id001
      reference_id: PMID:9200603
      supporting_text: Our results demonstrate that the eucaryal tRNA splicing endonuclease contains two
        functionally independent active sites for cleavage of the 5' and 3' splice sites, encoded by SEN2
        and SEN34, respectively.
- term:
    id: GO:0000214
    label: tRNA-intron endonuclease complex
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  qualifier: part_of
  supporting_entities:
  - InterPro:IPR042777
  review:
    summary: tRNA-intron endonuclease complex is supported.
    action: ACCEPT
    reason: The target is a SEN15-family protein, supporting transfer of the characterized four-subunit
      tRNA-splicing endonuclease role. SEN2 and SEN34 encode the cleavage active sites; Sen15 is a noncatalytic
      participant in intron recognition/excision. Participation in the overall cleavage-and-ligation pathway
      does not assert that Sen15 ligates RNA.
    supported_by:
    - *id001
- term: &id003
    id: GO:0000379
    label: tRNA-type intron splice site recognition and cleavage
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: involved_in
  supporting_entities:
  - PANTHER:PTN002003396
  - SGD:S000004663
  review:
    summary: tRNA-type intron splice site recognition and cleavage is supported.
    action: ACCEPT
    reason: The target is a SEN15-family protein, supporting transfer of the characterized four-subunit
      tRNA-splicing endonuclease role. SEN2 and SEN34 encode the cleavage active sites; Sen15 is a noncatalytic
      participant in intron recognition/excision. Participation in the overall cleavage-and-ligation pathway
      does not assert that Sen15 ligates RNA.
    supported_by:
    - *id001
- term:
    id: GO:0000379
    label: tRNA-type intron splice site recognition and cleavage
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  qualifier: involved_in
  supporting_entities:
  - InterPro:IPR042777
  review:
    summary: tRNA-type intron splice site recognition and cleavage is supported.
    action: ACCEPT
    reason: The target is a SEN15-family protein, supporting transfer of the characterized four-subunit
      tRNA-splicing endonuclease role. SEN2 and SEN34 encode the cleavage active sites; Sen15 is a noncatalytic
      participant in intron recognition/excision. Participation in the overall cleavage-and-ligation pathway
      does not assert that Sen15 ligates RNA.
    supported_by:
    - *id001
- term:
    id: GO:0003676
    label: nucleic acid binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  qualifier: enables
  supporting_entities:
  - InterPro:IPR011856
  review:
    summary: nucleic acid binding is not fully resolved.
    action: UNDECIDED
    reason: The Sen15 subunit supports an RNA-processing complex, but specific RNA contacts by the target
      or conserved contacts in a characterized Sen15 ortholog have not been established in the primary
      evidence inspected here. Complex membership alone does not resolve whether this particular subunit
      binds nucleic acid. Binding within an assembled complex would be sufficient; an isolated-subunit
      assay is not required.
    supported_by:
    - *id001
- term:
    id: GO:0005634
    label: nucleus
  evidence_type: HDA
  original_reference_id: PMID:16823372
  qualifier: is_active_in
  review:
    summary: nucleus is retained as an ancillary annotation.
    action: KEEP_AS_NON_CORE
    reason: Retain the PomBase localization observation from the fission yeast tagged-ORFeome screen.
      The accessible abstract documents the assay but not the individual localization image; the screen
      is not sufficient to identify which detected pool executes tRNA cleavage.
    supported_by:
    - &id002
      reference_id: PMID:16823372
      supporting_text: Next, we determined the localization of 4,431 proteins, corresponding to approximately
        90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: HDA
  original_reference_id: PMID:16823372
  qualifier: is_active_in
  review:
    summary: cytosol is retained as an ancillary annotation.
    action: KEEP_AS_NON_CORE
    reason: Retain the PomBase localization observation from the fission yeast tagged-ORFeome screen.
      The accessible abstract documents the assay but not the individual localization image; the screen
      is not sufficient to identify which detected pool executes tRNA cleavage.
    supported_by:
    - *id002
- term:
    id: GO:0006388
    label: tRNA splicing, via endonucleolytic cleavage and ligation
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  qualifier: involved_in
  supporting_entities:
  - InterPro:IPR036167
  review:
    summary: tRNA splicing, via endonucleolytic cleavage and ligation is supported.
    action: ACCEPT
    reason: The target is a SEN15-family protein, supporting transfer of the characterized four-subunit
      tRNA-splicing endonuclease role. SEN2 and SEN34 encode the cleavage active sites; Sen15 is a noncatalytic
      participant in intron recognition/excision. Participation in the overall cleavage-and-ligation pathway
      does not assert that Sen15 ligates RNA.
    supported_by:
    - *id001
references:
- id: GO_REF:0000002
  title: Gene Ontology annotation through association of InterPro records with GO terms
  findings: []
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings: []
- id: PMID:16823372
  title: ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces
    pombe.
  findings: []
  full_text_unavailable: true
- id: PMID:9200603
  title: 'The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous
    to the archaeal tRNA endonucleases.'
  findings: []
  full_text_unavailable: true
core_functions:
- description: Conserved noncatalytic participant in the tRNA-splicing endonuclease complex, supporting
    splice-site recognition and intron excision.
  supported_by:
  - *id001
  directly_involved_in:
  - *id003
  in_complex: *id004