id: Q7LKV3
gene_symbol: sen15
locus_tag: SPAC959.10
taxon:
  id: NCBITaxon:284812
  label: Schizosaccharomyces pombe (strain 972 / ATCC 24843)
status: COMPLETE
description: Sen15 participates noncatalytically in the tRNA-splicing endonuclease complex. The predicted
  tRNA-splicing process is correct and already established by curated annotations and conserved-family
  evidence.
source_documents:
- genes/SCHPO/sen15/sen15-protnlm-source.xml
- genes/SCHPO/sen15/sen15-uniprot.txt
- genes/SCHPO/sen15/sen15-ai-review.yaml
predictions:
- source_method: ProtNLM2
  source_version: pre-release post-processed-2026_02_28k.xml
  predicted_term:
    id: GO:0006388
    label: tRNA splicing, via endonucleolytic cleavage and ligation
  predicted_term_type: GO_BP
  review:
    assessment: CNN
    confidence_score: 2
    summary: The SEN15-family assignment of the target and the PAINT-curated endonuclease-complex and
      splice-site-recognition assertions support a conserved noncatalytic role in pre-tRNA intron excision.
      The characterized budding yeast complex contains four subunits, with cleavage active sites in Sen2
      and Sen34. Participation in the full splicing process does not require Sen15 itself to catalyze
      either cleavage or ligation. This exact process is already in GOA, and the corresponding endonuclease-participation
      biology is established, so the supported prediction is not novel.
    supported_by:
    - reference_id: PMID:9200603
      supporting_text: Our results demonstrate that the eucaryal tRNA splicing endonuclease contains two
        functionally independent active sites for cleavage of the 5' and 3' splice sites, encoded by SEN2
        and SEN34, respectively.
    - reference_id: file:SCHPO/sen15/sen15-uniprot.txt
      supporting_text: DR   InterPro; IPR042777; Sen15_fungi.
  source_reference_id: file:SCHPO/sen15/sen15-protnlm-source.xml
references:
- id: file:SCHPO/sen15/sen15-uniprot.txt
  title: 'UniProt record for sen15: specific family/domain assignment'
- id: PMID:9200603
  title: 'The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous
    to the archaeal tRNA endonucleases.'
- id: file:SCHPO/sen15/sen15-protnlm-source.xml
  title: Original pre-release ProtNLM2 XML entry for sen15