| Claim/finding | Evidence type (genetics, microscopy, EM, assay) | Key quantitative detail | Organism | Source (with year and URL) |
|---|---|---|---|---|
| **snx41** corresponds to a fission-yeast sorting nexin family member with **PX** and **BAR** domains, matching the UniProt O60107 annotation and supporting classification as a **PX-BAR sorting nexin** | Domain/bioinformatic background; review synthesis | PX domains are generally ~100–130 aa and bind phosphoinositides; BAR domains mediate dimerization/membrane curvature sensing and remodeling | *Schizosaccharomyces pombe* (target) with family-level conservation across yeasts | Hanley & Cooper, 2020, *Cells*, https://doi.org/10.3390/cells10010017; Popelka et al., 2017, *PNAS*, https://doi.org/10.1073/pnas.1708367114 (pqac-00000001, pqac-00000008, pqac-00000009) |
| In yeast SNX-BAR systems, **Snx41** belongs to the **Snx4-family** of sorting nexins that form distinct heterodimeric complexes with cargo/pathway specificity | Review/background from primary literature synthesis | Snx4 family comprises Snx4, Atg20/Snx42, and Snx41; distinct dimers have overlapping but nonidentical roles | Yeast (family-level, including fungi) | Hanley & Cooper, 2020, *Cells*, https://doi.org/10.3390/cells10010017 (pqac-00000000, pqac-00000002, pqac-00000004) |
| In the autophagy-edge model, **Snx41 forms a complex with Atg24 competitively with Atg20**, and this **Atg24–Atg20/Snx41** complex localizes to the **opening edge of the isolation membrane (IM)** | Live-cell fluorescence microscopy | Snx41 localization to the IM opening edge is reported; Atg24/Atg20 show ring-like signal at the edge of cup-shaped IMs | Budding yeast (*Saccharomyces cerevisiae*) | Kotani et al., 2023, *Nature Communications*, https://doi.org/10.1038/s41467-023-41525-x (pqac-00000005, pqac-00000010) |
| The Atg24–Atg20/Snx41 sorting nexin complex is required to keep the IM opening sufficiently large for **non-selective autophagic sequestration of large particles** | Genetics, microscopy, EM, autophagy cargo assays | Without the complex, IMs expand with a **small opening** that excludes particles larger than about **25 nm**, including ribosomes and proteasomes | Budding yeast (*Saccharomyces cerevisiae*) | Kotani et al., 2023, *Nature Communications*, https://doi.org/10.1038/s41467-023-41525-x (pqac-00000005, pqac-00000010, pqac-00000011) |
| **snx41Δ** alone causes milder defects than loss of **ATG24** or **atg20Δ snx41Δ** double deletion, indicating **partial redundancy** with Atg20 in the Atg24 complex | Genetics; viability assays; microscopy-based IM measurements | ATG24 knockout and **atg20Δ snx41Δ** strongly reduce viability under nitrogen starvation; **atg20Δ** is milder and **snx41Δ** shows no or minimal viability defect alone | Budding yeast (*Saccharomyces cerevisiae*) | Kotani et al., 2023, *Nature Communications*, https://doi.org/10.1038/s41467-023-41525-x (pqac-00000006, pqac-00000012) |
| The Atg24 complex is specifically important for degradation of **large** cytoplasmic assemblies, whereas small soluble markers can still be degraded without it | Autophagy assays; immunoblot readouts | Small markers such as **Pgk1–GFP** and ALP remain degradable; larger assemblies around **~20–25 nm** or more become increasingly dependent on the complex | Budding yeast (*Saccharomyces cerevisiae*) | Kotani et al., 2023, *Nature Communications*, https://doi.org/10.1038/s41467-023-41525-x (pqac-00000005, pqac-00000013) |
| Particle-size calibration experiments support a **size threshold** for Atg24-complex-dependent autophagy | Assay; particle engineering; immunoblot | Bacterial oligomer reporters estimated at **~20 nm (Dps–GFP)** and **~25 nm (RibH–GFP)** were used to define the entry threshold | Budding yeast (*Saccharomyces cerevisiae*) | Kotani et al., 2023, *Nature Communications*, https://doi.org/10.1038/s41467-023-41525-x (pqac-00000011, pqac-00000013) |
| Electron microscopy shows that in the absence of the Atg24 complex, autophagic bodies can form but are **depleted of ribosomes**, demonstrating failed capture of large complexes rather than complete autophagy arrest | Electron microscopy | In **atg24Δ** cells, autophagic bodies were present but were described as **strikingly absent from ribosomes**, unlike wild type | Budding yeast (*Saccharomyces cerevisiae*) | Kotani et al., 2023, *Nature Communications*, https://doi.org/10.1038/s41467-023-41525-x (pqac-00000005, pqac-00000010) |
| IM opening measurements directly show that **snx41Δ** alters opening-size behavior, although less strongly than more severe mutant combinations | Fluorescence microscopy; quantitative image analysis | Figure-associated boxplots report **n = 487 WT**, **483 atg20Δ**, **483 snx41Δ** IMs measured across **two independent experiments** | Budding yeast (*Saccharomyces cerevisiae*) | Kotani et al., 2023, *Nature Communications*, https://doi.org/10.1038/s41467-023-41525-x (pqac-00000006, pqac-00000014) |
| Expert synthesis places Snx41-family proteins at the intersection of **endosomal retrograde trafficking** and **autophagy/protein homeostasis**, explaining why direct biochemical data for fission-yeast Snx41 remain limited but family-based functional inference is strong | Expert review/analysis | Review notes cytosolic **Snx4-Atg20** and **Snx4-Snx41** heterodimers relocalize during stress and participate in protein-homeostasis pathways | Yeast and broader eukaryotic sorting nexin field | Hanley & Cooper, 2020, *Cells*, https://doi.org/10.3390/cells10010017 (pqac-00000000, pqac-00000001) |


*Table: This table summarizes evidence-supported findings relevant to Schizosaccharomyces pombe Snx41 and the Atg24–Atg20/Snx41 PX-BAR sorting nexin complex, emphasizing localization, autophagy function, and quantitative phenotypes. It is useful as a compact evidence map separating direct observations from family-level inference.*