id: Q7LL15
gene_symbol: sus1
product_type: PROTEIN
status: COMPLETE
taxon:
  id: NCBITaxon:284812
  label: Schizosaccharomyces pombe (strain 972 / ATCC 24843)
description: Sus1 is a conserved small subunit of the SAGA transcription coactivator and the TREX-2 mRNA
  export complex. It connects transcription-associated chromatin regulation with nuclear mRNA export.
  Within the SAGA deubiquitination module it supports complex assembly and histone H2B deubiquitination
  by the catalytic subunit; it is not itself a histone deubiquitinase.
existing_annotations:
- term: &id007
    id: GO:0000124
    label: SAGA complex
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: part_of
  supporting_entities:
  - FB:FBgn0000618
  - PANTHER:PTN000282153
  - PomBase:SPBC6B1.12c
  - SGD:S000028510
  - UniProtKB:Q9NPA8
  review:
    summary: SAGA complex is supported.
    action: ACCEPT
    reason: Fission yeast SAGA purification establishes Sus1 membership. In sgf73 deletion mutants, Sus1,
      Sgf11 and Ubp8 are absent from SAGA purifications, placing Sus1 within the Sgf73-anchored deubiquitination
      module. The conserved composition is also supported by the earlier target SAGA study.
    supported_by:
    - &id001
      reference_id: PMID:19056896
      supporting_text: Purification of the S. pombe SAGA complex showed that its subunit composition is
        identical to that of Saccharomyces cerevisiae.
    - reference_id: PMID:31748520
      supporting_text: Mass spectrometry analyses confirmed that, in S. pombe sgf73Δ mutants, the DUB
        subunits Ubp8, Sgf11 and Sus1 are absent from SAGA purifications
- term:
    id: GO:0000124
    label: SAGA complex
  evidence_type: IDA
  original_reference_id: PMID:19056896
  qualifier: part_of
  review:
    summary: SAGA complex is supported.
    action: ACCEPT
    reason: Fission yeast SAGA purification establishes Sus1 membership. In sgf73 deletion mutants, Sus1,
      Sgf11 and Ubp8 are absent from SAGA purifications, placing Sus1 within the Sgf73-anchored deubiquitination
      module. The conserved composition is also supported by the earlier target SAGA study.
    supported_by:
    - *id001
    - reference_id: PMID:31748520
      supporting_text: Mass spectrometry analyses confirmed that, in S. pombe sgf73Δ mutants, the DUB
        subunits Ubp8, Sgf11 and Sus1 are absent from SAGA purifications
- term:
    id: GO:0000124
    label: SAGA complex
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  qualifier: part_of
  supporting_entities:
  - InterPro:IPR018783
  - UniRule:UR000112776
  review:
    summary: SAGA complex is supported.
    action: ACCEPT
    reason: Fission yeast SAGA purification establishes Sus1 membership. In sgf73 deletion mutants, Sus1,
      Sgf11 and Ubp8 are absent from SAGA purifications, placing Sus1 within the Sgf73-anchored deubiquitination
      module. The conserved composition is also supported by the earlier target SAGA study.
    supported_by:
    - *id001
    - reference_id: PMID:31748520
      supporting_text: Mass spectrometry analyses confirmed that, in S. pombe sgf73Δ mutants, the DUB
        subunits Ubp8, Sgf11 and Sus1 are absent from SAGA purifications
- term:
    id: GO:0000124
    label: SAGA complex
  evidence_type: IPI
  original_reference_id: PMID:19056896
  qualifier: part_of
  review:
    summary: SAGA complex is supported.
    action: ACCEPT
    reason: Fission yeast SAGA purification establishes Sus1 membership. In sgf73 deletion mutants, Sus1,
      Sgf11 and Ubp8 are absent from SAGA purifications, placing Sus1 within the Sgf73-anchored deubiquitination
      module. The conserved composition is also supported by the earlier target SAGA study.
    supported_by:
    - *id001
    - reference_id: PMID:31748520
      supporting_text: Mass spectrometry analyses confirmed that, in S. pombe sgf73Δ mutants, the DUB
        subunits Ubp8, Sgf11 and Sus1 are absent from SAGA purifications
- term:
    id: GO:0000124
    label: SAGA complex
  evidence_type: IPI
  original_reference_id: PMID:29079657
  qualifier: part_of
  supporting_entities:
  - PomBase:SPBC887.18c
  review:
    summary: SAGA complex is supported.
    action: ACCEPT
    reason: Fission yeast SAGA purification establishes Sus1 membership. In sgf73 deletion mutants, Sus1,
      Sgf11 and Ubp8 are absent from SAGA purifications, placing Sus1 within the Sgf73-anchored deubiquitination
      module. The conserved composition is also supported by the earlier target SAGA study.
    supported_by:
    - *id001
    - reference_id: PMID:31748520
      supporting_text: Mass spectrometry analyses confirmed that, in S. pombe sgf73Δ mutants, the DUB
        subunits Ubp8, Sgf11 and Sus1 are absent from SAGA purifications
- term:
    id: GO:0000932
    label: P-body
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  supporting_entities:
  - UniProtKB-SubCell:SL-0230
  review:
    summary: P-body is not fully resolved.
    action: UNDECIDED
    reason: The reviewed primary evidence establishes nuclear SAGA and nuclear-pore export functions,
      but does not resolve a functional cytoplasmic or P-body pool of fission yeast Sus1. The automatic
      localization mapping is plausible but needs the underlying localization experiment or a specific
      justified transfer.
    supported_by:
    - &id002
      reference_id: PMID:14718168
      supporting_text: Biochemical analyses show that Sus1 interacts with SAGA, a large intranuclear histone
        acetylase complex involved in transcription initiation, and with the Sac3-Thp1 complex, which
        functions in mRNA export with specific nuclear pore proteins at the nuclear basket.
- term: &id006
    id: GO:0003713
    label: transcription coactivator activity
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: enables
  supporting_entities:
  - PANTHER:PTN000282153
  - UniProtKB:Q9NPA8
  review:
    summary: transcription coactivator activity is supported.
    action: ACCEPT
    reason: Sus1 is a subunit of the transcriptional coactivator SAGA. Its conserved role is supported
      by target complex purification and by transcription assays and promoter recruitment in characterized
      budding yeast Sus1; it does not imply Sus1 possesses an independent histone-modifying catalytic
      activity.
    supported_by:
    - *id001
    - *id002
- term:
    id: GO:0003713
    label: transcription coactivator activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  qualifier: enables
  supporting_entities:
  - InterPro:IPR018783
  - UniRule:UR000112776
  review:
    summary: transcription coactivator activity is supported.
    action: ACCEPT
    reason: Sus1 is a subunit of the transcriptional coactivator SAGA. Its conserved role is supported
      by target complex purification and by transcription assays and promoter recruitment in characterized
      budding yeast Sus1; it does not imply Sus1 possesses an independent histone-modifying catalytic
      activity.
    supported_by:
    - *id001
    - *id002
- term: &id008
    id: GO:0005634
    label: nucleus
  evidence_type: IEA
  original_reference_id: GO_REF:0000104
  qualifier: located_in
  supporting_entities:
  - UniRule:UR000112776
  review:
    summary: nucleus is supported.
    action: ACCEPT
    reason: Characterized Sus1 participates in the Sac3-Thp1/TREX-2 export machinery and concentrates
      at nuclear pores; sus1 mutants impair nuclear mRNA export. The conserved Sus1 subfamily and curated
      phylogenetic/orthology assertions support transfer of this functional complex context to fission
      yeast.
    supported_by:
    - *id002
- term:
    id: GO:0005643
    label: nuclear pore
  evidence_type: IEA
  original_reference_id: GO_REF:0000104
  qualifier: part_of
  supporting_entities:
  - UniRule:UR000112776
  review:
    summary: nuclear pore is supported.
    action: ACCEPT
    reason: Characterized Sus1 participates in the Sac3-Thp1/TREX-2 export machinery and concentrates
      at nuclear pores; sus1 mutants impair nuclear mRNA export. The conserved Sus1 subfamily and curated
      phylogenetic/orthology assertions support transfer of this functional complex context to fission
      yeast.
    supported_by:
    - *id002
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  supporting_entities:
  - UniProtKB-SubCell:SL-0190
  review:
    summary: nucleoplasm is supported.
    action: ACCEPT
    reason: Characterized Sus1 participates in the Sac3-Thp1/TREX-2 export machinery and concentrates
      at nuclear pores; sus1 mutants impair nuclear mRNA export. The conserved Sus1 subfamily and curated
      phylogenetic/orthology assertions support transfer of this functional complex context to fission
      yeast.
    supported_by:
    - *id002
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IEA
  original_reference_id: GO_REF:0000104
  qualifier: located_in
  supporting_entities:
  - UniRule:UR000112776
  review:
    summary: cytoplasm is not fully resolved.
    action: UNDECIDED
    reason: The reviewed primary evidence establishes nuclear SAGA and nuclear-pore export functions,
      but does not resolve a functional cytoplasmic or P-body pool of fission yeast Sus1. The automatic
      localization mapping is plausible but needs the underlying localization experiment or a specific
      justified transfer.
    supported_by:
    - *id002
- term: &id004
    id: GO:0006357
    label: regulation of transcription by RNA polymerase II
  evidence_type: EXP
  original_reference_id: PMID:21642955
  qualifier: involved_in
  review:
    summary: regulation of transcription by RNA polymerase II is supported.
    action: ACCEPT
    reason: Sus1 is a subunit of the transcriptional coactivator SAGA. Its conserved role is supported
      by target complex purification and by transcription assays and promoter recruitment in characterized
      budding yeast Sus1; it does not imply Sus1 possesses an independent histone-modifying catalytic
      activity. The original PMID:21642955 annotation is retained at its stated evidential scope; accessible
      source text and the independent evidence cited here are considered together.
    supported_by:
    - *id001
    - *id002
- term:
    id: GO:0006357
    label: regulation of transcription by RNA polymerase II
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: involved_in
  supporting_entities:
  - FB:FBgn0000618
  - PANTHER:PTN000282153
  - PomBase:SPBC6B1.12c
  - SGD:S000028510
  - UniProtKB:Q9NPA8
  review:
    summary: regulation of transcription by RNA polymerase II is supported.
    action: ACCEPT
    reason: Sus1 is a subunit of the transcriptional coactivator SAGA. Its conserved role is supported
      by target complex purification and by transcription assays and promoter recruitment in characterized
      budding yeast Sus1; it does not imply Sus1 possesses an independent histone-modifying catalytic
      activity.
    supported_by:
    - *id001
    - *id002
- term:
    id: GO:0006357
    label: regulation of transcription by RNA polymerase II
  evidence_type: NAS
  original_reference_id: PMID:19056896
  qualifier: involved_in
  review:
    summary: regulation of transcription by RNA polymerase II is supported.
    action: ACCEPT
    reason: Sus1 is a subunit of the transcriptional coactivator SAGA. Its conserved role is supported
      by target complex purification and by transcription assays and promoter recruitment in characterized
      budding yeast Sus1; it does not imply Sus1 possesses an independent histone-modifying catalytic
      activity.
    supported_by:
    - *id001
    - *id002
- term:
    id: GO:0006368
    label: transcription elongation by RNA polymerase II
  evidence_type: IEA
  original_reference_id: GO_REF:0000104
  qualifier: involved_in
  supporting_entities:
  - UniRule:UR000112776
  review:
    summary: transcription elongation by RNA polymerase II is supported.
    action: ACCEPT
    reason: Sus1 links SAGA-dependent transcription with export; the curated homology assertion of participation
      in elongation is compatible with its transcription-associated chromatin role. This is an accessory
      contribution to RNA polymerase II transcription, not RNA polymerase activity.
    supported_by:
    - *id002
    - &id003
      reference_id: PMID:16855026
      supporting_text: We demonstrate that Sus1 is involved in the SAGA-dependent histone H2B deubiquitinylation
        and maintenance of normal H3 methylation levels.
- term:
    id: GO:0006406
    label: mRNA export from nucleus
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  qualifier: involved_in
  supporting_entities:
  - InterPro:IPR018783
  - UniRule:UR000112776
  review:
    summary: mRNA export from nucleus is supported.
    action: ACCEPT
    reason: Characterized Sus1 participates in the Sac3-Thp1/TREX-2 export machinery and concentrates
      at nuclear pores; sus1 mutants impair nuclear mRNA export. The conserved Sus1 subfamily and curated
      phylogenetic/orthology assertions support transfer of this functional complex context to fission
      yeast.
    supported_by:
    - *id002
- term: &id009
    id: GO:0016973
    label: poly(A)+ mRNA export from nucleus
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: involved_in
  supporting_entities:
  - FB:FBgn0000618
  - PANTHER:PTN000282153
  - SGD:S000028510
  - UniProtKB:Q9NPA8
  review:
    summary: poly(A)+ mRNA export from nucleus is supported.
    action: ACCEPT
    reason: Characterized Sus1 participates in the Sac3-Thp1/TREX-2 export machinery and concentrates
      at nuclear pores; sus1 mutants impair nuclear mRNA export. The conserved Sus1 subfamily and curated
      phylogenetic/orthology assertions support transfer of this functional complex context to fission
      yeast.
    supported_by:
    - *id002
- term: &id005
    id: GO:0045815
    label: transcription initiation-coupled chromatin remodeling
  evidence_type: IC
  original_reference_id: GO_REF:0000111
  qualifier: involved_in
  supporting_entities:
  - GO:0000124
  review:
    summary: transcription initiation-coupled chromatin remodeling is supported.
    action: ACCEPT
    reason: Sus1 supports the SAGA histone H2B deubiquitination module through its association with Ubp8
      and Sgf11. This establishes a conserved contribution to chromatin regulation at transcription activation;
      the catalytic deubiquitinase is a separate subunit.
    supported_by:
    - *id003
    - *id001
- term:
    id: GO:0045893
    label: positive regulation of DNA-templated transcription
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  qualifier: involved_in
  supporting_entities:
  - InterPro:IPR018783
  - UniRule:UR000112776
  review:
    summary: positive regulation of DNA-templated transcription is supported.
    action: ACCEPT
    reason: Sus1 is a subunit of the transcriptional coactivator SAGA. Its conserved role is supported
      by target complex purification and by transcription assays and promoter recruitment in characterized
      budding yeast Sus1; it does not imply Sus1 possesses an independent histone-modifying catalytic
      activity.
    supported_by:
    - *id001
    - *id002
- term:
    id: GO:0045944
    label: positive regulation of transcription by RNA polymerase II
  evidence_type: ISO
  original_reference_id: GO_REF:0000024
  qualifier: involved_in
  supporting_entities:
  - SGD:S000028510
  review:
    summary: positive regulation of transcription by RNA polymerase II is supported.
    action: ACCEPT
    reason: Sus1 is a subunit of the transcriptional coactivator SAGA. Its conserved role is supported
      by target complex purification and by transcription assays and promoter recruitment in characterized
      budding yeast Sus1; it does not imply Sus1 possesses an independent histone-modifying catalytic
      activity.
    supported_by:
    - *id001
    - *id002
- term: &id010
    id: GO:0070390
    label: transcription export complex 2
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: part_of
  supporting_entities:
  - FB:FBgn0000618
  - PANTHER:PTN000282153
  - SGD:S000028510
  - UniProtKB:Q9NPA8
  review:
    summary: transcription export complex 2 is supported.
    action: ACCEPT
    reason: Characterized Sus1 participates in the Sac3-Thp1/TREX-2 export machinery and concentrates
      at nuclear pores; sus1 mutants impair nuclear mRNA export. The conserved Sus1 subfamily and curated
      phylogenetic/orthology assertions support transfer of this functional complex context to fission
      yeast.
    supported_by:
    - *id002
- term:
    id: GO:0070390
    label: transcription export complex 2
  evidence_type: IEA
  original_reference_id: GO_REF:0000104
  qualifier: part_of
  supporting_entities:
  - UniRule:UR000112776
  review:
    summary: transcription export complex 2 is supported.
    action: ACCEPT
    reason: Characterized Sus1 participates in the Sac3-Thp1/TREX-2 export machinery and concentrates
      at nuclear pores; sus1 mutants impair nuclear mRNA export. The conserved Sus1 subfamily and curated
      phylogenetic/orthology assertions support transfer of this functional complex context to fission
      yeast.
    supported_by:
    - *id002
- term:
    id: GO:0070390
    label: transcription export complex 2
  evidence_type: ISO
  original_reference_id: GO_REF:0000024
  qualifier: part_of
  supporting_entities:
  - SGD:S000028510
  review:
    summary: transcription export complex 2 is supported.
    action: ACCEPT
    reason: Characterized Sus1 participates in the Sac3-Thp1/TREX-2 export machinery and concentrates
      at nuclear pores; sus1 mutants impair nuclear mRNA export. The conserved Sus1 subfamily and curated
      phylogenetic/orthology assertions support transfer of this functional complex context to fission
      yeast.
    supported_by:
    - *id002
- term:
    id: GO:0071819
    label: DUBm complex
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: part_of
  supporting_entities:
  - AGI_LocusCode:AT3G27100
  - FB:FBgn0000618
  - PANTHER:PTN000282153
  - SGD:S000028510
  - UniProtKB:Q9NPA8
  review:
    summary: DUBm complex is supported.
    action: ACCEPT
    reason: Sus1 supports the SAGA histone H2B deubiquitination module through its association with Ubp8
      and Sgf11. This establishes a conserved contribution to chromatin regulation at transcription activation;
      the catalytic deubiquitinase is a separate subunit.
    supported_by:
    - *id003
    - *id001
- term:
    id: GO:0071819
    label: DUBm complex
  evidence_type: IEA
  original_reference_id: GO_REF:0000104
  qualifier: part_of
  supporting_entities:
  - UniRule:UR000112776
  review:
    summary: DUBm complex is supported.
    action: ACCEPT
    reason: Sus1 supports the SAGA histone H2B deubiquitination module through its association with Ubp8
      and Sgf11. This establishes a conserved contribution to chromatin regulation at transcription activation;
      the catalytic deubiquitinase is a separate subunit.
    supported_by:
    - *id003
    - *id001
references:
- id: GO_REF:0000024
  title: Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator
    judgment of sequence similarity
  findings: []
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings: []
- id: GO_REF:0000044
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping,
    accompanied by conservative changes to GO terms applied by UniProt
  findings: []
- id: GO_REF:0000104
  title: Electronic Gene Ontology annotations created by transferring manual GO annotations between related
    proteins based on shared sequence features
  findings: []
- id: GO_REF:0000111
  title: Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity
    (ISS) annotation to support the inference
  findings: []
- id: GO_REF:0000120
  title: Combined Automated Annotation using Multiple IEA Methods
  findings: []
- id: PMID:19056896
  title: The S. pombe SAGA complex controls the switch from proliferation to sexual differentiation through
    the opposing roles of its subunits Gcn5 and Spt8.
  findings: []
  full_text_unavailable: true
- id: PMID:21642955
  title: Tra1 has specific regulatory roles, rather than global functions, within the SAGA co-activator
    complex.
  findings: []
  full_text_unavailable: true
- id: PMID:29079657
  title: TORC1 and TORC2 converge to regulate the SAGA co-activator in response to nutrient availability.
  findings: []
  full_text_unavailable: true
- id: PMID:14718168
  title: Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated
    mRNA export machinery.
  findings: []
  full_text_unavailable: true
- id: PMID:16855026
  title: The mRNA export factor Sus1 is involved in Spt/Ada/Gcn5 acetyltransferase-mediated H2B deubiquitinylation
    through its interaction with Ubp8 and Sgf11.
  findings: []
  full_text_unavailable: true
- id: PMID:31748520
  title: Chaperone-mediated ordered assembly of the SAGA and NuA4 transcription co-activator complexes
    in yeast.
  reference_review:
    relevance: HIGH
    correctness: VERIFIED
    review_notes: Full text and PubMed identity verified. Target SAGA purifications and sgf73 deletion
      establish Sus1 within the DUB module; the paper cautions that its small size limits MS quantification.
core_functions:
- description: SAGA coactivator subunit supporting transcription-associated chromatin regulation.
  supported_by:
  - *id001
  - *id003
  directly_involved_in:
  - *id004
  - *id005
  contributes_to_molecular_function: *id006
  in_complex: *id007
  locations:
  - *id008
- description: Conserved TREX-2 component supporting export of polyadenylated mRNA through nuclear pores.
  supported_by:
  - *id002
  directly_involved_in:
  - *id009
  in_complex: *id010
  locations:
  - *id008