Tpi1 (SPCC24B10.21; also called tpi) is the triose-phosphate isomerase of the fission yeast Schizosaccharomyces pombe, a 249-residue TIM-barrel enzyme that is a homodimer by similarity. It catalyses the reversible isomerisation of dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate, allowing both triose phosphates produced by aldolase to proceed through lower glycolysis and balancing the triose pool during gluconeogenesis. The S. pombe gene complements a S. cerevisiae tpi1 mutant, and the protein localises to the cytosol.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004807 triose-phosphate isomerase activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT IBA triose-phosphate isomerase activity; tpi1 is a canonical TIM-family member and sole TIM of S. pombe. Supporting Evidence: file:SCHPO/tpi1/tpi1-uniprot.txt Reaction=D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate; |
| GO:0004807 triose-phosphate isomerase activity | IEA GO_REF:0000120 | ACCEPT | Summary: EC 5.3.1.1 / RHEA:18585 automated mapping; correct. Supporting Evidence: file:SCHPO/tpi1/tpi1-uniprot.txt Reaction=D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate; |
| GO:0004807 triose-phosphate isomerase activity | IGI PMID:3912263 Transcription of the triose-phosphate-isomerase gene of Schi... | ACCEPT | Summary: Russell (1985) cloned S. pombe tpi by functional complementation of a S. cerevisiae tpi1 mutant (with/from SGD TPI1), showing the S. pombe gene supplies triose-phosphate isomerase function in vivo. Abstract-only in cache; consistent with PomBase curation. Supporting Evidence: PMID:3912263 Gene tpi, encoding the glycolytic enzyme triose phosphate isomerase (TPI) from the fission yeast Schizosaccharomyces pombe was cloned by complementation of a Saccharomyces cerevisiae tpil mutant. |
| GO:0005829 cytosol | HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ... | ACCEPT | Summary: ORFeome YFP localisation places Tpi1 in the cytosol. Supporting Evidence: PMID:16823372 we determined the localization of 4,431 proteins |
| GO:0006094 gluconeogenesis | IBA GO_REF:0000033 | ACCEPT | Summary: IBA gluconeogenesis; the TIM reaction is freely reversible and the sole TIM is required to balance DHAP and GAP during gluconeogenesis. Consistent with the S. cerevisiae TPI1 review. Supporting Evidence: file:SCHPO/tpi1/tpi1-uniprot.txt PATHWAY: Carbohydrate biosynthesis; gluconeogenesis. |
| GO:0006094 gluconeogenesis | IEA GO_REF:0000041 | ACCEPT | Summary: UniPathway mapping to gluconeogenesis; correct. Supporting Evidence: file:SCHPO/tpi1/tpi1-uniprot.txt PATHWAY: Carbohydrate biosynthesis; gluconeogenesis. |
| GO:0006096 glycolysis | IBA GO_REF:0000033 | ACCEPT | Summary: IBA glycolysis; Tpi1 converts aldolase-derived DHAP to GAP, channelling both trioses into lower glycolysis. Supporting Evidence: file:SCHPO/tpi1/tpi1-uniprot.txt PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3- PMID:3912263 Gene tpi, encoding the glycolytic enzyme triose phosphate isomerase (TPI) from the fission yeast Schizosaccharomyces pombe was cloned by complementation of a Saccharomyces cerevisiae tpil mutant. |
| GO:0006096 glycolysis | IEA GO_REF:0000120 | ACCEPT | Summary: UniPathway/combined automated mapping to glycolysis; correct. Supporting Evidence: file:SCHPO/tpi1/tpi1-uniprot.txt PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3- |
| GO:0046166 glyceraldehyde-3-phosphate biosynthetic process | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: IBA glyceraldehyde-3-phosphate biosynthetic process; describes the glycolytic direction (DHAP to GAP) of the TIM reaction. Correct at step level but redundant with glycolysis; non-core, as in the S. cerevisiae TPI1 review. Supporting Evidence: file:SCHPO/tpi1/tpi1-uniprot.txt Reaction=D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate; |
| GO:0061621 obsolete canonical glycolysis | IGI PMID:3912263 Transcription of the triose-phosphate-isomerase gene of Schi... | MODIFY | Summary: PomBase IGI (complementation of S. cerevisiae tpi1) to 'canonical glycolysis', obsoleted by GO on 2026-09-01 and replaced_by glycolysis (GO:0006096). Biology correct; term should be updated. Reason: Obsolete term; GO replaced_by GO:0006096. Proposed replacements: glycolysis Supporting Evidence: PMID:3912263 Gene tpi, encoding the glycolytic enzyme triose phosphate isomerase (TPI) from the fission yeast Schizosaccharomyces pombe was cloned by complementation of a Saccharomyces cerevisiae tpil mutant. |
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