id: Q9P7N2
gene_symbol: vas2
locus_tag: SPAP27G11.06c
taxon:
  id: NCBITaxon:284812
  label: Schizosaccharomyces pombe (strain 972 / ATCC 24843)
status: COMPLETE
description: Vas2/Aps1 supports AP-1-mediated membrane-protein sorting and exit transport from endosomes.
  Intracellular protein transport is supported but less precise than the established cargo-transport routes.
source_documents:
- genes/SCHPO/vas2/vas2-protnlm-source.xml
- genes/SCHPO/vas2/vas2-uniprot.txt
- genes/SCHPO/vas2/vas2-ai-review.yaml
predictions:
- source_method: ProtNLM2
  source_version: pre-release post-processed-2026_02_28k.xml
  predicted_term:
    id: GO:0006886
    label: intracellular protein transport
  predicted_term_type: GO_BP
  review:
    assessment: LSP
    confidence_score: 2
    summary: Fission yeast Aps1/Vas2 deletion and pull-down experiments establish AP-1 assembly and exit
      transport from endosomes, including trafficking of the membrane protein Syb1. GOA already records
      endosome-to-Golgi and endosome-to-plasma-membrane protein transport. The predicted intracellular
      protein transport is correct but less precise than those established routes. The unrelated ARBA
      intercellular-transport assertion means movement between cells and is not evidence supporting this
      assessment.
    supported_by:
    - reference_id: PMID:19624755
      supporting_text: In pull-down assay, Apm1 binds Apl2 even in the absence of Aps1 and Apl4, and Apl4
        binds Aps1 even in the absence of Apm1 and Apl2. Consistently, the deletion of any subunit generally
        caused the disassociation of the heterotetrameric complex from endosomes, although some subunits
        weakly localized to endosomes. In addition, the deletion of individual subunits caused similar
        endosomal accumulation of v-SNARE synaptobrevin Syb1. Altogether, results suggest that the four
        subunits are all essential for the heterotetrameric complex formation and for the AP-1 function
        in exit transport from endosomes.
  source_reference_id: file:SCHPO/vas2/vas2-protnlm-source.xml
references:
- id: PMID:19624755
  title: Deletion mutants of AP-1 adaptin subunits display distinct phenotypes in fission yeast.
- id: file:SCHPO/vas2/vas2-protnlm-source.xml
  title: Original pre-release ProtNLM2 XML entry for vas2